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Programmed cell death in yeast by thionin-like peptide from Capsicum annuum fruits involving activation of caspases and extracellular H(+) flux

CaThi is a thionin-like peptide isolated from fruits of Capsicum annuum, which has strong antimicrobial activity against bacteria, yeasts and filamentous fungi, and induced reactive oxygen species (ROS) in fungi. ROS are molecules that appear in the early stages of programmed cell death or apoptosis...

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Autores principales: Taveira, Gabriel B., Mello, Érica O., Souza, Sávio B., Monteiro, Renan M., Ramos, Alessandro C., Carvalho, André O., Rodrigues, Rosana, Okorokov, Lev A., Gomes, Valdirene M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920138/
https://www.ncbi.nlm.nih.gov/pubmed/29599127
http://dx.doi.org/10.1042/BSR20180119
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author Taveira, Gabriel B.
Mello, Érica O.
Souza, Sávio B.
Monteiro, Renan M.
Ramos, Alessandro C.
Carvalho, André O.
Rodrigues, Rosana
Okorokov, Lev A.
Gomes, Valdirene M.
author_facet Taveira, Gabriel B.
Mello, Érica O.
Souza, Sávio B.
Monteiro, Renan M.
Ramos, Alessandro C.
Carvalho, André O.
Rodrigues, Rosana
Okorokov, Lev A.
Gomes, Valdirene M.
author_sort Taveira, Gabriel B.
collection PubMed
description CaThi is a thionin-like peptide isolated from fruits of Capsicum annuum, which has strong antimicrobial activity against bacteria, yeasts and filamentous fungi, and induced reactive oxygen species (ROS) in fungi. ROS are molecules that appear in the early stages of programmed cell death or apoptosis in fungi. Due to this fact, in this work we analyzed some events that may be related to process of apoptosis on yeast induced by CaThi. To investigate this possibility, we evaluated phosphatidylserine (PS) externalization, presence of active caspases and the ability of CaThi to bind to DNA in Candida tropicalis cells. Additionally, we investigated mitochondrial membrane potential, cell surface pH, and extracellular H(+) fluxes in C. tropicalis cells after treatment with CaThi. Our results showed that CaThi induced PS externalization in the outer leaflet of the cell membrane, activation of caspases, and it had the ability for DNA binding and to dissipate mitochondrial membrane potential. In addition, the cell surface pH increased significantly when the C. tropicalis cells were exposed to CaThi which corroborates with ~96% inhibition on extracellular H(+) efflux. Taking together, these data suggest that this peptide is capable of promoting an imbalance in pH homeostasis during yeast cell death playing a modulatory role in the H(+) transport systems. In conclusion, our results strongly indicated that CaThi triggers apoptosis in C. tropicalis cells, involving a pH signaling mechanism.
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spelling pubmed-59201382018-05-01 Programmed cell death in yeast by thionin-like peptide from Capsicum annuum fruits involving activation of caspases and extracellular H(+) flux Taveira, Gabriel B. Mello, Érica O. Souza, Sávio B. Monteiro, Renan M. Ramos, Alessandro C. Carvalho, André O. Rodrigues, Rosana Okorokov, Lev A. Gomes, Valdirene M. Biosci Rep Research Articles CaThi is a thionin-like peptide isolated from fruits of Capsicum annuum, which has strong antimicrobial activity against bacteria, yeasts and filamentous fungi, and induced reactive oxygen species (ROS) in fungi. ROS are molecules that appear in the early stages of programmed cell death or apoptosis in fungi. Due to this fact, in this work we analyzed some events that may be related to process of apoptosis on yeast induced by CaThi. To investigate this possibility, we evaluated phosphatidylserine (PS) externalization, presence of active caspases and the ability of CaThi to bind to DNA in Candida tropicalis cells. Additionally, we investigated mitochondrial membrane potential, cell surface pH, and extracellular H(+) fluxes in C. tropicalis cells after treatment with CaThi. Our results showed that CaThi induced PS externalization in the outer leaflet of the cell membrane, activation of caspases, and it had the ability for DNA binding and to dissipate mitochondrial membrane potential. In addition, the cell surface pH increased significantly when the C. tropicalis cells were exposed to CaThi which corroborates with ~96% inhibition on extracellular H(+) efflux. Taking together, these data suggest that this peptide is capable of promoting an imbalance in pH homeostasis during yeast cell death playing a modulatory role in the H(+) transport systems. In conclusion, our results strongly indicated that CaThi triggers apoptosis in C. tropicalis cells, involving a pH signaling mechanism. Portland Press Ltd. 2018-04-27 /pmc/articles/PMC5920138/ /pubmed/29599127 http://dx.doi.org/10.1042/BSR20180119 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Taveira, Gabriel B.
Mello, Érica O.
Souza, Sávio B.
Monteiro, Renan M.
Ramos, Alessandro C.
Carvalho, André O.
Rodrigues, Rosana
Okorokov, Lev A.
Gomes, Valdirene M.
Programmed cell death in yeast by thionin-like peptide from Capsicum annuum fruits involving activation of caspases and extracellular H(+) flux
title Programmed cell death in yeast by thionin-like peptide from Capsicum annuum fruits involving activation of caspases and extracellular H(+) flux
title_full Programmed cell death in yeast by thionin-like peptide from Capsicum annuum fruits involving activation of caspases and extracellular H(+) flux
title_fullStr Programmed cell death in yeast by thionin-like peptide from Capsicum annuum fruits involving activation of caspases and extracellular H(+) flux
title_full_unstemmed Programmed cell death in yeast by thionin-like peptide from Capsicum annuum fruits involving activation of caspases and extracellular H(+) flux
title_short Programmed cell death in yeast by thionin-like peptide from Capsicum annuum fruits involving activation of caspases and extracellular H(+) flux
title_sort programmed cell death in yeast by thionin-like peptide from capsicum annuum fruits involving activation of caspases and extracellular h(+) flux
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920138/
https://www.ncbi.nlm.nih.gov/pubmed/29599127
http://dx.doi.org/10.1042/BSR20180119
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