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The bHLH transcription factor GmPIB1 facilitates resistance to Phytophthora sojae in Glycine max

Phytophthora sojae Kaufmann and Gerdemann causes Phytophthora root rot, a destructive soybean disease worldwide. A basic helix–loop–helix (bHLH) transcription factor is thought to be involved in the response to P. sojae infection in soybean, as revealed by RNA sequencing (RNA-seq). However, the mole...

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Autores principales: Cheng, Qun, Dong, Lidong, Gao, Tianjiao, Liu, Tengfei, Li, Ninghui, Wang, Le, Chang, Xin, Wu, Junjiang, Xu, Pengfei, Zhang, Shuzhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920285/
https://www.ncbi.nlm.nih.gov/pubmed/29579245
http://dx.doi.org/10.1093/jxb/ery103
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author Cheng, Qun
Dong, Lidong
Gao, Tianjiao
Liu, Tengfei
Li, Ninghui
Wang, Le
Chang, Xin
Wu, Junjiang
Xu, Pengfei
Zhang, Shuzhen
author_facet Cheng, Qun
Dong, Lidong
Gao, Tianjiao
Liu, Tengfei
Li, Ninghui
Wang, Le
Chang, Xin
Wu, Junjiang
Xu, Pengfei
Zhang, Shuzhen
author_sort Cheng, Qun
collection PubMed
description Phytophthora sojae Kaufmann and Gerdemann causes Phytophthora root rot, a destructive soybean disease worldwide. A basic helix–loop–helix (bHLH) transcription factor is thought to be involved in the response to P. sojae infection in soybean, as revealed by RNA sequencing (RNA-seq). However, the molecular mechanism underlying this response is currently unclear. Here, we explored the function and underlying mechanisms of a bHLH transcription factor in soybean, designated GmPIB1 (P. sojae-inducible bHLH transcription factor), during host responses to P. sojae. GmPIB1 was significantly induced by P. sojae in the resistant soybean cultivar ‘L77-1863’. Analysis of transgenic soybean hairy roots with elevated or reduced expression of GmPIB1 demonstrated that GmPIB1 enhances resistance to P. sojae and reduces reactive oxygen species (ROS) accumulation. Quantitative reverse transcription PCR and chromatin immunoprecipitation–quantitative PCR assays revealed that GmPIB1 binds directly to the promoter of GmSPOD1 and represses its expression; this gene encodes a key enzyme in ROS production. Moreover, transgenic soybean hairy roots with GmSPOD1 silencing through RNA interference exhibited improved resistance to P. sojae and reduced ROS generation. These findings suggest that GmPIB1 enhances resistance to P. sojae by repressing the expression of GmSPOD1.
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spelling pubmed-59202852018-05-04 The bHLH transcription factor GmPIB1 facilitates resistance to Phytophthora sojae in Glycine max Cheng, Qun Dong, Lidong Gao, Tianjiao Liu, Tengfei Li, Ninghui Wang, Le Chang, Xin Wu, Junjiang Xu, Pengfei Zhang, Shuzhen J Exp Bot Research Papers Phytophthora sojae Kaufmann and Gerdemann causes Phytophthora root rot, a destructive soybean disease worldwide. A basic helix–loop–helix (bHLH) transcription factor is thought to be involved in the response to P. sojae infection in soybean, as revealed by RNA sequencing (RNA-seq). However, the molecular mechanism underlying this response is currently unclear. Here, we explored the function and underlying mechanisms of a bHLH transcription factor in soybean, designated GmPIB1 (P. sojae-inducible bHLH transcription factor), during host responses to P. sojae. GmPIB1 was significantly induced by P. sojae in the resistant soybean cultivar ‘L77-1863’. Analysis of transgenic soybean hairy roots with elevated or reduced expression of GmPIB1 demonstrated that GmPIB1 enhances resistance to P. sojae and reduces reactive oxygen species (ROS) accumulation. Quantitative reverse transcription PCR and chromatin immunoprecipitation–quantitative PCR assays revealed that GmPIB1 binds directly to the promoter of GmSPOD1 and represses its expression; this gene encodes a key enzyme in ROS production. Moreover, transgenic soybean hairy roots with GmSPOD1 silencing through RNA interference exhibited improved resistance to P. sojae and reduced ROS generation. These findings suggest that GmPIB1 enhances resistance to P. sojae by repressing the expression of GmSPOD1. Oxford University Press 2018-04-27 2018-03-22 /pmc/articles/PMC5920285/ /pubmed/29579245 http://dx.doi.org/10.1093/jxb/ery103 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Cheng, Qun
Dong, Lidong
Gao, Tianjiao
Liu, Tengfei
Li, Ninghui
Wang, Le
Chang, Xin
Wu, Junjiang
Xu, Pengfei
Zhang, Shuzhen
The bHLH transcription factor GmPIB1 facilitates resistance to Phytophthora sojae in Glycine max
title The bHLH transcription factor GmPIB1 facilitates resistance to Phytophthora sojae in Glycine max
title_full The bHLH transcription factor GmPIB1 facilitates resistance to Phytophthora sojae in Glycine max
title_fullStr The bHLH transcription factor GmPIB1 facilitates resistance to Phytophthora sojae in Glycine max
title_full_unstemmed The bHLH transcription factor GmPIB1 facilitates resistance to Phytophthora sojae in Glycine max
title_short The bHLH transcription factor GmPIB1 facilitates resistance to Phytophthora sojae in Glycine max
title_sort bhlh transcription factor gmpib1 facilitates resistance to phytophthora sojae in glycine max
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920285/
https://www.ncbi.nlm.nih.gov/pubmed/29579245
http://dx.doi.org/10.1093/jxb/ery103
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