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Quantitative assessment of gene promoter methylation in non-small cell lung cancer using methylation-sensitive high-resolution melting

DNA methylation is closely associated with aberrant epigenetic changes. Previous studies have identified various genes associated with non-small cell lung cancer (NSCLC), but the precise combination responsible for its etiology is still debated. The aim of the present study was to select a new set o...

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Autores principales: Liu, Fangming, Zhang, Honglian, Lu, Shaohua, Wu, Zhenhua, Zhou, Lin, Cheng, Zule, Bai, Yanan, Zhao, Jianlong, Zhang, Qiqing, Mao, Hongju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920472/
https://www.ncbi.nlm.nih.gov/pubmed/29725463
http://dx.doi.org/10.3892/ol.2018.8321
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author Liu, Fangming
Zhang, Honglian
Lu, Shaohua
Wu, Zhenhua
Zhou, Lin
Cheng, Zule
Bai, Yanan
Zhao, Jianlong
Zhang, Qiqing
Mao, Hongju
author_facet Liu, Fangming
Zhang, Honglian
Lu, Shaohua
Wu, Zhenhua
Zhou, Lin
Cheng, Zule
Bai, Yanan
Zhao, Jianlong
Zhang, Qiqing
Mao, Hongju
author_sort Liu, Fangming
collection PubMed
description DNA methylation is closely associated with aberrant epigenetic changes. Previous studies have identified various genes associated with non-small cell lung cancer (NSCLC), but the precise combination responsible for its etiology is still debated. The aim of the present study was to select a new set of NSCLC-related genes using methylation-sensitive high-resolution melting. The promoter methylation status of six selected genes, consisting of protocadherin γ subfamily B, 6 (PCDHGB6), homeobox A9 (HOXA9), O(6)-methylguanine-DNA methyltransferase (MGMT), microRNA (miR)-126, suppressor of cytokine signaling 3 (SOCS3) and Ras association domain family member 5, also termed NORE1A, was evaluated in 54 NSCLC patients. From these samples, genome-wide DNA was extracted and bisulfite conversion was performed along with fluorogenic quantitative polymerase chain reaction to detect methylation values of the six selected promoters. The present results revealed frequent methylation on PCDHGB6, HOXA9 and miR-126, which contrasted with infrequent methylation on MGMT. The results indicated no methylation on either SOCS3 or NORE1A. The sensitivity and specificity of the methylation assessment were 85.2 and 81.5%, respectively, and the analysis results were validated by pyrosequencing. Furthermore, minute comparison of the association between DNA methylation and clinical features was performed. Overall, these results may provide potential information for the development of better clinical diagnostics and more targeted and effective therapies for NSCLC.
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spelling pubmed-59204722018-05-03 Quantitative assessment of gene promoter methylation in non-small cell lung cancer using methylation-sensitive high-resolution melting Liu, Fangming Zhang, Honglian Lu, Shaohua Wu, Zhenhua Zhou, Lin Cheng, Zule Bai, Yanan Zhao, Jianlong Zhang, Qiqing Mao, Hongju Oncol Lett Articles DNA methylation is closely associated with aberrant epigenetic changes. Previous studies have identified various genes associated with non-small cell lung cancer (NSCLC), but the precise combination responsible for its etiology is still debated. The aim of the present study was to select a new set of NSCLC-related genes using methylation-sensitive high-resolution melting. The promoter methylation status of six selected genes, consisting of protocadherin γ subfamily B, 6 (PCDHGB6), homeobox A9 (HOXA9), O(6)-methylguanine-DNA methyltransferase (MGMT), microRNA (miR)-126, suppressor of cytokine signaling 3 (SOCS3) and Ras association domain family member 5, also termed NORE1A, was evaluated in 54 NSCLC patients. From these samples, genome-wide DNA was extracted and bisulfite conversion was performed along with fluorogenic quantitative polymerase chain reaction to detect methylation values of the six selected promoters. The present results revealed frequent methylation on PCDHGB6, HOXA9 and miR-126, which contrasted with infrequent methylation on MGMT. The results indicated no methylation on either SOCS3 or NORE1A. The sensitivity and specificity of the methylation assessment were 85.2 and 81.5%, respectively, and the analysis results were validated by pyrosequencing. Furthermore, minute comparison of the association between DNA methylation and clinical features was performed. Overall, these results may provide potential information for the development of better clinical diagnostics and more targeted and effective therapies for NSCLC. D.A. Spandidos 2018-05 2018-03-22 /pmc/articles/PMC5920472/ /pubmed/29725463 http://dx.doi.org/10.3892/ol.2018.8321 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Liu, Fangming
Zhang, Honglian
Lu, Shaohua
Wu, Zhenhua
Zhou, Lin
Cheng, Zule
Bai, Yanan
Zhao, Jianlong
Zhang, Qiqing
Mao, Hongju
Quantitative assessment of gene promoter methylation in non-small cell lung cancer using methylation-sensitive high-resolution melting
title Quantitative assessment of gene promoter methylation in non-small cell lung cancer using methylation-sensitive high-resolution melting
title_full Quantitative assessment of gene promoter methylation in non-small cell lung cancer using methylation-sensitive high-resolution melting
title_fullStr Quantitative assessment of gene promoter methylation in non-small cell lung cancer using methylation-sensitive high-resolution melting
title_full_unstemmed Quantitative assessment of gene promoter methylation in non-small cell lung cancer using methylation-sensitive high-resolution melting
title_short Quantitative assessment of gene promoter methylation in non-small cell lung cancer using methylation-sensitive high-resolution melting
title_sort quantitative assessment of gene promoter methylation in non-small cell lung cancer using methylation-sensitive high-resolution melting
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920472/
https://www.ncbi.nlm.nih.gov/pubmed/29725463
http://dx.doi.org/10.3892/ol.2018.8321
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