Cargando…

Quantitative Detection of Ultraviolet Light‐induced Photoproducts in Mouse Skin by Immunohistochemistry

UVB‐induced cyclobutane pyrimidine dimers (CPDs) and pyrimidine‐pyrimidone (6‐4)photoproducts [(6‐4)photoproducts] in mouse skin DNA were quantitatively measured using an immunohistochemical approach with a computer‐aided color image analyzer. The skins of the C3H/HeN mice were irradiated with ultra...

Descripción completa

Detalles Bibliográficos
Autores principales: Qin, Xiusheng, Zhang, Shaomin, Oda, Hideaki, Nakatsuru, Yoko, Shimizu, Seiichiro, Yamazaki, Yukari, Nikaido, Osamu, Ishikawa, Takatoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1995
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920636/
https://www.ncbi.nlm.nih.gov/pubmed/8567394
http://dx.doi.org/10.1111/j.1349-7006.1995.tb03018.x
_version_ 1783317867185307648
author Qin, Xiusheng
Zhang, Shaomin
Oda, Hideaki
Nakatsuru, Yoko
Shimizu, Seiichiro
Yamazaki, Yukari
Nikaido, Osamu
Ishikawa, Takatoshi
author_facet Qin, Xiusheng
Zhang, Shaomin
Oda, Hideaki
Nakatsuru, Yoko
Shimizu, Seiichiro
Yamazaki, Yukari
Nikaido, Osamu
Ishikawa, Takatoshi
author_sort Qin, Xiusheng
collection PubMed
description UVB‐induced cyclobutane pyrimidine dimers (CPDs) and pyrimidine‐pyrimidone (6‐4)photoproducts [(6‐4)photoproducts] in mouse skin DNA were quantitatively measured using an immunohistochemical approach with a computer‐aided color image analyzer. The skins of the C3H/HeN mice were irradiated with ultraviolet B (UV‐B, 280‐320 nm), and processed to give conventional formalin‐fixed, paraffin‐embedded histologic sections. Routine immunohistochemistry clearly demonstrated a dosedependent induction of both photoproducts. CPDs were detectable at doses ġ 125 J/m(2), while for (6‐4)photoproducts, the minimal dose at which they were detectable was 250 J/m(1) in the present study. A time course study showed that the repair of (6‐4)photoproducts was more rapid than that of CPDs, and that epidermal cells bad a higher capacity for their removal than dermal cells. About half of the (6‐4)photoproducts were excised within the first 24 h after the irradiation, and the process was essentially complete by 72 h. In contrast, there was no apparent removal (less than 10%) of CPDs in the first 24 h and they only completely disappeared from the epidermal cells at 120 h after irradiation. The effect of DNA dilution due to increased turnover of epidermal cells after UV‐B irradiation was evaluated by quantitative immunohistochemical measurement of the time course of bromodeoxyuridine (BrdUrd) incorporated into nuclei at 2 days post irradiation when the proliferation reaches a peak. The removal of photoproducts was more marked than the decrease in BrdUrd staining. Our results suggest that mouse skin cells can repair both (6‐4)photoproducts and CPDs, but with considerably lower efficiency, especially in the latter case, than human or monkey skin cells.
format Online
Article
Text
id pubmed-5920636
institution National Center for Biotechnology Information
language English
publishDate 1995
publisher Blackwell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-59206362018-05-11 Quantitative Detection of Ultraviolet Light‐induced Photoproducts in Mouse Skin by Immunohistochemistry Qin, Xiusheng Zhang, Shaomin Oda, Hideaki Nakatsuru, Yoko Shimizu, Seiichiro Yamazaki, Yukari Nikaido, Osamu Ishikawa, Takatoshi Jpn J Cancer Res Article UVB‐induced cyclobutane pyrimidine dimers (CPDs) and pyrimidine‐pyrimidone (6‐4)photoproducts [(6‐4)photoproducts] in mouse skin DNA were quantitatively measured using an immunohistochemical approach with a computer‐aided color image analyzer. The skins of the C3H/HeN mice were irradiated with ultraviolet B (UV‐B, 280‐320 nm), and processed to give conventional formalin‐fixed, paraffin‐embedded histologic sections. Routine immunohistochemistry clearly demonstrated a dosedependent induction of both photoproducts. CPDs were detectable at doses ġ 125 J/m(2), while for (6‐4)photoproducts, the minimal dose at which they were detectable was 250 J/m(1) in the present study. A time course study showed that the repair of (6‐4)photoproducts was more rapid than that of CPDs, and that epidermal cells bad a higher capacity for their removal than dermal cells. About half of the (6‐4)photoproducts were excised within the first 24 h after the irradiation, and the process was essentially complete by 72 h. In contrast, there was no apparent removal (less than 10%) of CPDs in the first 24 h and they only completely disappeared from the epidermal cells at 120 h after irradiation. The effect of DNA dilution due to increased turnover of epidermal cells after UV‐B irradiation was evaluated by quantitative immunohistochemical measurement of the time course of bromodeoxyuridine (BrdUrd) incorporated into nuclei at 2 days post irradiation when the proliferation reaches a peak. The removal of photoproducts was more marked than the decrease in BrdUrd staining. Our results suggest that mouse skin cells can repair both (6‐4)photoproducts and CPDs, but with considerably lower efficiency, especially in the latter case, than human or monkey skin cells. Blackwell Publishing Ltd 1995-11 /pmc/articles/PMC5920636/ /pubmed/8567394 http://dx.doi.org/10.1111/j.1349-7006.1995.tb03018.x Text en
spellingShingle Article
Qin, Xiusheng
Zhang, Shaomin
Oda, Hideaki
Nakatsuru, Yoko
Shimizu, Seiichiro
Yamazaki, Yukari
Nikaido, Osamu
Ishikawa, Takatoshi
Quantitative Detection of Ultraviolet Light‐induced Photoproducts in Mouse Skin by Immunohistochemistry
title Quantitative Detection of Ultraviolet Light‐induced Photoproducts in Mouse Skin by Immunohistochemistry
title_full Quantitative Detection of Ultraviolet Light‐induced Photoproducts in Mouse Skin by Immunohistochemistry
title_fullStr Quantitative Detection of Ultraviolet Light‐induced Photoproducts in Mouse Skin by Immunohistochemistry
title_full_unstemmed Quantitative Detection of Ultraviolet Light‐induced Photoproducts in Mouse Skin by Immunohistochemistry
title_short Quantitative Detection of Ultraviolet Light‐induced Photoproducts in Mouse Skin by Immunohistochemistry
title_sort quantitative detection of ultraviolet light‐induced photoproducts in mouse skin by immunohistochemistry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920636/
https://www.ncbi.nlm.nih.gov/pubmed/8567394
http://dx.doi.org/10.1111/j.1349-7006.1995.tb03018.x
work_keys_str_mv AT qinxiusheng quantitativedetectionofultravioletlightinducedphotoproductsinmouseskinbyimmunohistochemistry
AT zhangshaomin quantitativedetectionofultravioletlightinducedphotoproductsinmouseskinbyimmunohistochemistry
AT odahideaki quantitativedetectionofultravioletlightinducedphotoproductsinmouseskinbyimmunohistochemistry
AT nakatsuruyoko quantitativedetectionofultravioletlightinducedphotoproductsinmouseskinbyimmunohistochemistry
AT shimizuseiichiro quantitativedetectionofultravioletlightinducedphotoproductsinmouseskinbyimmunohistochemistry
AT yamazakiyukari quantitativedetectionofultravioletlightinducedphotoproductsinmouseskinbyimmunohistochemistry
AT nikaidoosamu quantitativedetectionofultravioletlightinducedphotoproductsinmouseskinbyimmunohistochemistry
AT ishikawatakatoshi quantitativedetectionofultravioletlightinducedphotoproductsinmouseskinbyimmunohistochemistry