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Sevoflurane-induced memory impairment in the postnatal developing mouse brain

The aim of the present study was to confirm that sevoflurane induces memory impairment in the postnatal developing mouse brain and determine its mechanism of action. C57BL/6 mice 7 days old were randomly assigned into a 2.6% sevoflurane (n=68), a 1.3% sevoflurane (n=68) and a control (n=38) group. B...

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Autores principales: Lu, Zhijun, Sun, Jihui, Xin, Yichun, Chen, Ken, Ding, Wen, Wang, Yujia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920718/
https://www.ncbi.nlm.nih.gov/pubmed/29731813
http://dx.doi.org/10.3892/etm.2018.5950
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author Lu, Zhijun
Sun, Jihui
Xin, Yichun
Chen, Ken
Ding, Wen
Wang, Yujia
author_facet Lu, Zhijun
Sun, Jihui
Xin, Yichun
Chen, Ken
Ding, Wen
Wang, Yujia
author_sort Lu, Zhijun
collection PubMed
description The aim of the present study was to confirm that sevoflurane induces memory impairment in the postnatal developing mouse brain and determine its mechanism of action. C57BL/6 mice 7 days old were randomly assigned into a 2.6% sevoflurane (n=68), a 1.3% sevoflurane (n=68) and a control (n=38) group. Blood gas analysis was performed to evaluate hypoxia and respiratory depression during anesthesia in 78 mice. Measurements for expression of caspase-3 by immunohistochemistry, cleavage of poly adenosine diphosphate-ribose polymerase (PARP) by western blotting, as well as levels of brain-derived neurotrophic factor (BDNF), tyrosine kinase receptor type 2 (Ntrk2), pro-BDNF, p75 neurotrophin receptor (p75NTR) and protein kinase B (PKB/Akt) by enzyme-linked immunosorbent assay were performed in the hippocampus of 12 mice from each group. A total of 60 mice underwent the Morris water maze (MWM) test. Results from the MWM test indicated that the time spent in the northwest quadrant and platform site crossovers by mice in the 2.6 and 1.3% sevoflurane groups was significantly lower than that of the control group. Meanwhile, levels of caspase-3 and cleaved PARP in the 2.6 and 1.3% sevoflurane groups were significantly higher than that in the control group. Levels of pro-BDNF and p75NTR were significantly increased and the level of PKB/Akt was significantly decreased following exposure to 2.6% sevoflurane. Finally, the memory of postnatal mice was impaired by sevoflurane, this was determined using a MWM test. Therefore, the results of the current study suggest that caspase-3 induced cleavage of PARP, as well as pro-BDNF, p75NTR and PKB/Akt may be important in sevoflurane-induced memory impairment in the postnatal developing mouse brain.
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spelling pubmed-59207182018-05-04 Sevoflurane-induced memory impairment in the postnatal developing mouse brain Lu, Zhijun Sun, Jihui Xin, Yichun Chen, Ken Ding, Wen Wang, Yujia Exp Ther Med Articles The aim of the present study was to confirm that sevoflurane induces memory impairment in the postnatal developing mouse brain and determine its mechanism of action. C57BL/6 mice 7 days old were randomly assigned into a 2.6% sevoflurane (n=68), a 1.3% sevoflurane (n=68) and a control (n=38) group. Blood gas analysis was performed to evaluate hypoxia and respiratory depression during anesthesia in 78 mice. Measurements for expression of caspase-3 by immunohistochemistry, cleavage of poly adenosine diphosphate-ribose polymerase (PARP) by western blotting, as well as levels of brain-derived neurotrophic factor (BDNF), tyrosine kinase receptor type 2 (Ntrk2), pro-BDNF, p75 neurotrophin receptor (p75NTR) and protein kinase B (PKB/Akt) by enzyme-linked immunosorbent assay were performed in the hippocampus of 12 mice from each group. A total of 60 mice underwent the Morris water maze (MWM) test. Results from the MWM test indicated that the time spent in the northwest quadrant and platform site crossovers by mice in the 2.6 and 1.3% sevoflurane groups was significantly lower than that of the control group. Meanwhile, levels of caspase-3 and cleaved PARP in the 2.6 and 1.3% sevoflurane groups were significantly higher than that in the control group. Levels of pro-BDNF and p75NTR were significantly increased and the level of PKB/Akt was significantly decreased following exposure to 2.6% sevoflurane. Finally, the memory of postnatal mice was impaired by sevoflurane, this was determined using a MWM test. Therefore, the results of the current study suggest that caspase-3 induced cleavage of PARP, as well as pro-BDNF, p75NTR and PKB/Akt may be important in sevoflurane-induced memory impairment in the postnatal developing mouse brain. D.A. Spandidos 2018-05 2018-03-12 /pmc/articles/PMC5920718/ /pubmed/29731813 http://dx.doi.org/10.3892/etm.2018.5950 Text en Copyright: © Lu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Lu, Zhijun
Sun, Jihui
Xin, Yichun
Chen, Ken
Ding, Wen
Wang, Yujia
Sevoflurane-induced memory impairment in the postnatal developing mouse brain
title Sevoflurane-induced memory impairment in the postnatal developing mouse brain
title_full Sevoflurane-induced memory impairment in the postnatal developing mouse brain
title_fullStr Sevoflurane-induced memory impairment in the postnatal developing mouse brain
title_full_unstemmed Sevoflurane-induced memory impairment in the postnatal developing mouse brain
title_short Sevoflurane-induced memory impairment in the postnatal developing mouse brain
title_sort sevoflurane-induced memory impairment in the postnatal developing mouse brain
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920718/
https://www.ncbi.nlm.nih.gov/pubmed/29731813
http://dx.doi.org/10.3892/etm.2018.5950
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