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Sevoflurane-induced memory impairment in the postnatal developing mouse brain
The aim of the present study was to confirm that sevoflurane induces memory impairment in the postnatal developing mouse brain and determine its mechanism of action. C57BL/6 mice 7 days old were randomly assigned into a 2.6% sevoflurane (n=68), a 1.3% sevoflurane (n=68) and a control (n=38) group. B...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920718/ https://www.ncbi.nlm.nih.gov/pubmed/29731813 http://dx.doi.org/10.3892/etm.2018.5950 |
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author | Lu, Zhijun Sun, Jihui Xin, Yichun Chen, Ken Ding, Wen Wang, Yujia |
author_facet | Lu, Zhijun Sun, Jihui Xin, Yichun Chen, Ken Ding, Wen Wang, Yujia |
author_sort | Lu, Zhijun |
collection | PubMed |
description | The aim of the present study was to confirm that sevoflurane induces memory impairment in the postnatal developing mouse brain and determine its mechanism of action. C57BL/6 mice 7 days old were randomly assigned into a 2.6% sevoflurane (n=68), a 1.3% sevoflurane (n=68) and a control (n=38) group. Blood gas analysis was performed to evaluate hypoxia and respiratory depression during anesthesia in 78 mice. Measurements for expression of caspase-3 by immunohistochemistry, cleavage of poly adenosine diphosphate-ribose polymerase (PARP) by western blotting, as well as levels of brain-derived neurotrophic factor (BDNF), tyrosine kinase receptor type 2 (Ntrk2), pro-BDNF, p75 neurotrophin receptor (p75NTR) and protein kinase B (PKB/Akt) by enzyme-linked immunosorbent assay were performed in the hippocampus of 12 mice from each group. A total of 60 mice underwent the Morris water maze (MWM) test. Results from the MWM test indicated that the time spent in the northwest quadrant and platform site crossovers by mice in the 2.6 and 1.3% sevoflurane groups was significantly lower than that of the control group. Meanwhile, levels of caspase-3 and cleaved PARP in the 2.6 and 1.3% sevoflurane groups were significantly higher than that in the control group. Levels of pro-BDNF and p75NTR were significantly increased and the level of PKB/Akt was significantly decreased following exposure to 2.6% sevoflurane. Finally, the memory of postnatal mice was impaired by sevoflurane, this was determined using a MWM test. Therefore, the results of the current study suggest that caspase-3 induced cleavage of PARP, as well as pro-BDNF, p75NTR and PKB/Akt may be important in sevoflurane-induced memory impairment in the postnatal developing mouse brain. |
format | Online Article Text |
id | pubmed-5920718 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-59207182018-05-04 Sevoflurane-induced memory impairment in the postnatal developing mouse brain Lu, Zhijun Sun, Jihui Xin, Yichun Chen, Ken Ding, Wen Wang, Yujia Exp Ther Med Articles The aim of the present study was to confirm that sevoflurane induces memory impairment in the postnatal developing mouse brain and determine its mechanism of action. C57BL/6 mice 7 days old were randomly assigned into a 2.6% sevoflurane (n=68), a 1.3% sevoflurane (n=68) and a control (n=38) group. Blood gas analysis was performed to evaluate hypoxia and respiratory depression during anesthesia in 78 mice. Measurements for expression of caspase-3 by immunohistochemistry, cleavage of poly adenosine diphosphate-ribose polymerase (PARP) by western blotting, as well as levels of brain-derived neurotrophic factor (BDNF), tyrosine kinase receptor type 2 (Ntrk2), pro-BDNF, p75 neurotrophin receptor (p75NTR) and protein kinase B (PKB/Akt) by enzyme-linked immunosorbent assay were performed in the hippocampus of 12 mice from each group. A total of 60 mice underwent the Morris water maze (MWM) test. Results from the MWM test indicated that the time spent in the northwest quadrant and platform site crossovers by mice in the 2.6 and 1.3% sevoflurane groups was significantly lower than that of the control group. Meanwhile, levels of caspase-3 and cleaved PARP in the 2.6 and 1.3% sevoflurane groups were significantly higher than that in the control group. Levels of pro-BDNF and p75NTR were significantly increased and the level of PKB/Akt was significantly decreased following exposure to 2.6% sevoflurane. Finally, the memory of postnatal mice was impaired by sevoflurane, this was determined using a MWM test. Therefore, the results of the current study suggest that caspase-3 induced cleavage of PARP, as well as pro-BDNF, p75NTR and PKB/Akt may be important in sevoflurane-induced memory impairment in the postnatal developing mouse brain. D.A. Spandidos 2018-05 2018-03-12 /pmc/articles/PMC5920718/ /pubmed/29731813 http://dx.doi.org/10.3892/etm.2018.5950 Text en Copyright: © Lu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Lu, Zhijun Sun, Jihui Xin, Yichun Chen, Ken Ding, Wen Wang, Yujia Sevoflurane-induced memory impairment in the postnatal developing mouse brain |
title | Sevoflurane-induced memory impairment in the postnatal developing mouse brain |
title_full | Sevoflurane-induced memory impairment in the postnatal developing mouse brain |
title_fullStr | Sevoflurane-induced memory impairment in the postnatal developing mouse brain |
title_full_unstemmed | Sevoflurane-induced memory impairment in the postnatal developing mouse brain |
title_short | Sevoflurane-induced memory impairment in the postnatal developing mouse brain |
title_sort | sevoflurane-induced memory impairment in the postnatal developing mouse brain |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920718/ https://www.ncbi.nlm.nih.gov/pubmed/29731813 http://dx.doi.org/10.3892/etm.2018.5950 |
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