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Comparison of the Chase Effects of Avidin, Streptavidin, Neutravidin, and Avidin‐Ferritin on a Radiolabeled Biotinylated Anti‐tumor Monoclonal Antibody
Injection of avidin can decrease the background radioactivity due to a radiolabeled biotinylated monoclonal antibody. We compared the chase effects of avidin, streptavidin, neutravidin, and avidin‐conjugated ferritin on a radiolabeled antitumor monoclonal antibody in tumor‐bearing nude mice. A radio...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
1995
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920811/ https://www.ncbi.nlm.nih.gov/pubmed/7744702 http://dx.doi.org/10.1111/j.1349-7006.1995.tb03056.x |
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author | Kobayashi, Hisataka Sakahara, Harumi Endo, Keigo Hosono, Makoto Yao, Zheng‐Sheng Toyama, Sakuji Konishi, Junji |
author_facet | Kobayashi, Hisataka Sakahara, Harumi Endo, Keigo Hosono, Makoto Yao, Zheng‐Sheng Toyama, Sakuji Konishi, Junji |
author_sort | Kobayashi, Hisataka |
collection | PubMed |
description | Injection of avidin can decrease the background radioactivity due to a radiolabeled biotinylated monoclonal antibody. We compared the chase effects of avidin, streptavidin, neutravidin, and avidin‐conjugated ferritin on a radiolabeled antitumor monoclonal antibody in tumor‐bearing nude mice. A radioiodine‐labeled biotinylated monoclonal antibody (OST7) was administered to athymic mice bearing osteogenic sarcomas. After 24 h, an avidin, streptavidin, neutravidin or avidin‐conjugated ferritin chaser was intravenously injected into the mice. At 2 h after the chase, the biodistribution of the radiolabeled monoclonal antibody was determined. Clearance from the blood was dose‐dependently accelerated by avidin and its effect was 10‐fold stronger than that of neutravidin or avidinferritin. Streptavidin did not promote clearance of the biotinylated antibody. Avidin was the most effective chasing agent for improving the biodistribution of the radiolabeled biotinylated monoclonal antibody among the four avidin derivatives tested. |
format | Online Article Text |
id | pubmed-5920811 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1995 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59208112018-05-11 Comparison of the Chase Effects of Avidin, Streptavidin, Neutravidin, and Avidin‐Ferritin on a Radiolabeled Biotinylated Anti‐tumor Monoclonal Antibody Kobayashi, Hisataka Sakahara, Harumi Endo, Keigo Hosono, Makoto Yao, Zheng‐Sheng Toyama, Sakuji Konishi, Junji Jpn J Cancer Res Article Injection of avidin can decrease the background radioactivity due to a radiolabeled biotinylated monoclonal antibody. We compared the chase effects of avidin, streptavidin, neutravidin, and avidin‐conjugated ferritin on a radiolabeled antitumor monoclonal antibody in tumor‐bearing nude mice. A radioiodine‐labeled biotinylated monoclonal antibody (OST7) was administered to athymic mice bearing osteogenic sarcomas. After 24 h, an avidin, streptavidin, neutravidin or avidin‐conjugated ferritin chaser was intravenously injected into the mice. At 2 h after the chase, the biodistribution of the radiolabeled monoclonal antibody was determined. Clearance from the blood was dose‐dependently accelerated by avidin and its effect was 10‐fold stronger than that of neutravidin or avidinferritin. Streptavidin did not promote clearance of the biotinylated antibody. Avidin was the most effective chasing agent for improving the biodistribution of the radiolabeled biotinylated monoclonal antibody among the four avidin derivatives tested. Blackwell Publishing Ltd 1995-03 /pmc/articles/PMC5920811/ /pubmed/7744702 http://dx.doi.org/10.1111/j.1349-7006.1995.tb03056.x Text en |
spellingShingle | Article Kobayashi, Hisataka Sakahara, Harumi Endo, Keigo Hosono, Makoto Yao, Zheng‐Sheng Toyama, Sakuji Konishi, Junji Comparison of the Chase Effects of Avidin, Streptavidin, Neutravidin, and Avidin‐Ferritin on a Radiolabeled Biotinylated Anti‐tumor Monoclonal Antibody |
title | Comparison of the Chase Effects of Avidin, Streptavidin, Neutravidin, and Avidin‐Ferritin on a Radiolabeled Biotinylated Anti‐tumor Monoclonal Antibody |
title_full | Comparison of the Chase Effects of Avidin, Streptavidin, Neutravidin, and Avidin‐Ferritin on a Radiolabeled Biotinylated Anti‐tumor Monoclonal Antibody |
title_fullStr | Comparison of the Chase Effects of Avidin, Streptavidin, Neutravidin, and Avidin‐Ferritin on a Radiolabeled Biotinylated Anti‐tumor Monoclonal Antibody |
title_full_unstemmed | Comparison of the Chase Effects of Avidin, Streptavidin, Neutravidin, and Avidin‐Ferritin on a Radiolabeled Biotinylated Anti‐tumor Monoclonal Antibody |
title_short | Comparison of the Chase Effects of Avidin, Streptavidin, Neutravidin, and Avidin‐Ferritin on a Radiolabeled Biotinylated Anti‐tumor Monoclonal Antibody |
title_sort | comparison of the chase effects of avidin, streptavidin, neutravidin, and avidin‐ferritin on a radiolabeled biotinylated anti‐tumor monoclonal antibody |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920811/ https://www.ncbi.nlm.nih.gov/pubmed/7744702 http://dx.doi.org/10.1111/j.1349-7006.1995.tb03056.x |
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