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Comparison of the Chase Effects of Avidin, Streptavidin, Neutravidin, and Avidin‐Ferritin on a Radiolabeled Biotinylated Anti‐tumor Monoclonal Antibody

Injection of avidin can decrease the background radioactivity due to a radiolabeled biotinylated monoclonal antibody. We compared the chase effects of avidin, streptavidin, neutravidin, and avidin‐conjugated ferritin on a radiolabeled antitumor monoclonal antibody in tumor‐bearing nude mice. A radio...

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Detalles Bibliográficos
Autores principales: Kobayashi, Hisataka, Sakahara, Harumi, Endo, Keigo, Hosono, Makoto, Yao, Zheng‐Sheng, Toyama, Sakuji, Konishi, Junji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1995
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920811/
https://www.ncbi.nlm.nih.gov/pubmed/7744702
http://dx.doi.org/10.1111/j.1349-7006.1995.tb03056.x
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author Kobayashi, Hisataka
Sakahara, Harumi
Endo, Keigo
Hosono, Makoto
Yao, Zheng‐Sheng
Toyama, Sakuji
Konishi, Junji
author_facet Kobayashi, Hisataka
Sakahara, Harumi
Endo, Keigo
Hosono, Makoto
Yao, Zheng‐Sheng
Toyama, Sakuji
Konishi, Junji
author_sort Kobayashi, Hisataka
collection PubMed
description Injection of avidin can decrease the background radioactivity due to a radiolabeled biotinylated monoclonal antibody. We compared the chase effects of avidin, streptavidin, neutravidin, and avidin‐conjugated ferritin on a radiolabeled antitumor monoclonal antibody in tumor‐bearing nude mice. A radioiodine‐labeled biotinylated monoclonal antibody (OST7) was administered to athymic mice bearing osteogenic sarcomas. After 24 h, an avidin, streptavidin, neutravidin or avidin‐conjugated ferritin chaser was intravenously injected into the mice. At 2 h after the chase, the biodistribution of the radiolabeled monoclonal antibody was determined. Clearance from the blood was dose‐dependently accelerated by avidin and its effect was 10‐fold stronger than that of neutravidin or avidinferritin. Streptavidin did not promote clearance of the biotinylated antibody. Avidin was the most effective chasing agent for improving the biodistribution of the radiolabeled biotinylated monoclonal antibody among the four avidin derivatives tested.
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spelling pubmed-59208112018-05-11 Comparison of the Chase Effects of Avidin, Streptavidin, Neutravidin, and Avidin‐Ferritin on a Radiolabeled Biotinylated Anti‐tumor Monoclonal Antibody Kobayashi, Hisataka Sakahara, Harumi Endo, Keigo Hosono, Makoto Yao, Zheng‐Sheng Toyama, Sakuji Konishi, Junji Jpn J Cancer Res Article Injection of avidin can decrease the background radioactivity due to a radiolabeled biotinylated monoclonal antibody. We compared the chase effects of avidin, streptavidin, neutravidin, and avidin‐conjugated ferritin on a radiolabeled antitumor monoclonal antibody in tumor‐bearing nude mice. A radioiodine‐labeled biotinylated monoclonal antibody (OST7) was administered to athymic mice bearing osteogenic sarcomas. After 24 h, an avidin, streptavidin, neutravidin or avidin‐conjugated ferritin chaser was intravenously injected into the mice. At 2 h after the chase, the biodistribution of the radiolabeled monoclonal antibody was determined. Clearance from the blood was dose‐dependently accelerated by avidin and its effect was 10‐fold stronger than that of neutravidin or avidinferritin. Streptavidin did not promote clearance of the biotinylated antibody. Avidin was the most effective chasing agent for improving the biodistribution of the radiolabeled biotinylated monoclonal antibody among the four avidin derivatives tested. Blackwell Publishing Ltd 1995-03 /pmc/articles/PMC5920811/ /pubmed/7744702 http://dx.doi.org/10.1111/j.1349-7006.1995.tb03056.x Text en
spellingShingle Article
Kobayashi, Hisataka
Sakahara, Harumi
Endo, Keigo
Hosono, Makoto
Yao, Zheng‐Sheng
Toyama, Sakuji
Konishi, Junji
Comparison of the Chase Effects of Avidin, Streptavidin, Neutravidin, and Avidin‐Ferritin on a Radiolabeled Biotinylated Anti‐tumor Monoclonal Antibody
title Comparison of the Chase Effects of Avidin, Streptavidin, Neutravidin, and Avidin‐Ferritin on a Radiolabeled Biotinylated Anti‐tumor Monoclonal Antibody
title_full Comparison of the Chase Effects of Avidin, Streptavidin, Neutravidin, and Avidin‐Ferritin on a Radiolabeled Biotinylated Anti‐tumor Monoclonal Antibody
title_fullStr Comparison of the Chase Effects of Avidin, Streptavidin, Neutravidin, and Avidin‐Ferritin on a Radiolabeled Biotinylated Anti‐tumor Monoclonal Antibody
title_full_unstemmed Comparison of the Chase Effects of Avidin, Streptavidin, Neutravidin, and Avidin‐Ferritin on a Radiolabeled Biotinylated Anti‐tumor Monoclonal Antibody
title_short Comparison of the Chase Effects of Avidin, Streptavidin, Neutravidin, and Avidin‐Ferritin on a Radiolabeled Biotinylated Anti‐tumor Monoclonal Antibody
title_sort comparison of the chase effects of avidin, streptavidin, neutravidin, and avidin‐ferritin on a radiolabeled biotinylated anti‐tumor monoclonal antibody
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920811/
https://www.ncbi.nlm.nih.gov/pubmed/7744702
http://dx.doi.org/10.1111/j.1349-7006.1995.tb03056.x
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