Tyrosine Phosphorylation of the Proto‐oncogene Product Vav and Its Association with the Adapter Grb2/Ash in a Human Leukemia Cell Line UT‐7

The vav proto‐oncogene product (Vav) is expressed exclusively in hematopoietic cells and is reported to have guanine nucleotide exchange activity. Here we report that granulocyte‐macrophage colonystimulating factor, interleukin‐3, and erythropoietin induce tyrosine phosphorylation of Vav in a human...

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Detalles Bibliográficos
Autores principales: Hanazono, Yutaka, Sasaki, Ko, Odai, Hideharu, Mimura, Toshihide, Mitani, Kinuko, Yazaki, Yoshio, Hirai, Hisamaru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1995
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920837/
https://www.ncbi.nlm.nih.gov/pubmed/7539782
http://dx.doi.org/10.1111/j.1349-7006.1995.tb03061.x
Descripción
Sumario:The vav proto‐oncogene product (Vav) is expressed exclusively in hematopoietic cells and is reported to have guanine nucleotide exchange activity. Here we report that granulocyte‐macrophage colonystimulating factor, interleukin‐3, and erythropoietin induce tyrosine phosphorylation of Vav in a human leukemia cell line UT‐7. Tyrosine phosphorylation of Vav is rapid and transient; it occurs within 1 min of the stimulation and at physiological concentrations of the factors. Furthermore, we show that Vav is constitutively associated with the adapter molecule Grb2/Ash in UT‐7. These data suggest that tryosine kinases, the adapter Grb2/Ash, and the guanine nucleotide exchange factor Vav are members of a signaling pathway leading to Ras activation in hematopoietic cells.

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