Tyrosine Phosphorylation of the Proto‐oncogene Product Vav and Its Association with the Adapter Grb2/Ash in a Human Leukemia Cell Line UT‐7

The vav proto‐oncogene product (Vav) is expressed exclusively in hematopoietic cells and is reported to have guanine nucleotide exchange activity. Here we report that granulocyte‐macrophage colonystimulating factor, interleukin‐3, and erythropoietin induce tyrosine phosphorylation of Vav in a human...

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Autores principales: Hanazono, Yutaka, Sasaki, Ko, Odai, Hideharu, Mimura, Toshihide, Mitani, Kinuko, Yazaki, Yoshio, Hirai, Hisamaru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1995
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920837/
https://www.ncbi.nlm.nih.gov/pubmed/7539782
http://dx.doi.org/10.1111/j.1349-7006.1995.tb03061.x
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author Hanazono, Yutaka
Sasaki, Ko
Odai, Hideharu
Mimura, Toshihide
Mitani, Kinuko
Yazaki, Yoshio
Hirai, Hisamaru
author_facet Hanazono, Yutaka
Sasaki, Ko
Odai, Hideharu
Mimura, Toshihide
Mitani, Kinuko
Yazaki, Yoshio
Hirai, Hisamaru
author_sort Hanazono, Yutaka
collection PubMed
description The vav proto‐oncogene product (Vav) is expressed exclusively in hematopoietic cells and is reported to have guanine nucleotide exchange activity. Here we report that granulocyte‐macrophage colonystimulating factor, interleukin‐3, and erythropoietin induce tyrosine phosphorylation of Vav in a human leukemia cell line UT‐7. Tyrosine phosphorylation of Vav is rapid and transient; it occurs within 1 min of the stimulation and at physiological concentrations of the factors. Furthermore, we show that Vav is constitutively associated with the adapter molecule Grb2/Ash in UT‐7. These data suggest that tryosine kinases, the adapter Grb2/Ash, and the guanine nucleotide exchange factor Vav are members of a signaling pathway leading to Ras activation in hematopoietic cells.
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spelling pubmed-59208372018-05-11 Tyrosine Phosphorylation of the Proto‐oncogene Product Vav and Its Association with the Adapter Grb2/Ash in a Human Leukemia Cell Line UT‐7 Hanazono, Yutaka Sasaki, Ko Odai, Hideharu Mimura, Toshihide Mitani, Kinuko Yazaki, Yoshio Hirai, Hisamaru Jpn J Cancer Res Rapid Communication The vav proto‐oncogene product (Vav) is expressed exclusively in hematopoietic cells and is reported to have guanine nucleotide exchange activity. Here we report that granulocyte‐macrophage colonystimulating factor, interleukin‐3, and erythropoietin induce tyrosine phosphorylation of Vav in a human leukemia cell line UT‐7. Tyrosine phosphorylation of Vav is rapid and transient; it occurs within 1 min of the stimulation and at physiological concentrations of the factors. Furthermore, we show that Vav is constitutively associated with the adapter molecule Grb2/Ash in UT‐7. These data suggest that tryosine kinases, the adapter Grb2/Ash, and the guanine nucleotide exchange factor Vav are members of a signaling pathway leading to Ras activation in hematopoietic cells. Blackwell Publishing Ltd 1995-04 /pmc/articles/PMC5920837/ /pubmed/7539782 http://dx.doi.org/10.1111/j.1349-7006.1995.tb03061.x Text en
spellingShingle Rapid Communication
Hanazono, Yutaka
Sasaki, Ko
Odai, Hideharu
Mimura, Toshihide
Mitani, Kinuko
Yazaki, Yoshio
Hirai, Hisamaru
Tyrosine Phosphorylation of the Proto‐oncogene Product Vav and Its Association with the Adapter Grb2/Ash in a Human Leukemia Cell Line UT‐7
title Tyrosine Phosphorylation of the Proto‐oncogene Product Vav and Its Association with the Adapter Grb2/Ash in a Human Leukemia Cell Line UT‐7
title_full Tyrosine Phosphorylation of the Proto‐oncogene Product Vav and Its Association with the Adapter Grb2/Ash in a Human Leukemia Cell Line UT‐7
title_fullStr Tyrosine Phosphorylation of the Proto‐oncogene Product Vav and Its Association with the Adapter Grb2/Ash in a Human Leukemia Cell Line UT‐7
title_full_unstemmed Tyrosine Phosphorylation of the Proto‐oncogene Product Vav and Its Association with the Adapter Grb2/Ash in a Human Leukemia Cell Line UT‐7
title_short Tyrosine Phosphorylation of the Proto‐oncogene Product Vav and Its Association with the Adapter Grb2/Ash in a Human Leukemia Cell Line UT‐7
title_sort tyrosine phosphorylation of the proto‐oncogene product vav and its association with the adapter grb2/ash in a human leukemia cell line ut‐7
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920837/
https://www.ncbi.nlm.nih.gov/pubmed/7539782
http://dx.doi.org/10.1111/j.1349-7006.1995.tb03061.x
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