Changes in Radiation Sensitivity of Human Osteosarcoma Cells after p53 Introduction

Human osteosarcoma SAOS‐2 cells, which have a deletion in p53 gene, were transfected with plasmid pMSVneop53 containing human p53 cDNA and neomycin‐resistance gene. Three clones (SAOS‐MC10, SAOS‐MC11 and SAOS‐MC43) among 60 clones expressed p53 mRNA. No p53 protein was observed in SAOS‐MC10, while S...

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Detalles Bibliográficos
Autores principales: Miyakoshi, Junji, Yamagishi, Nobuyuki, Ohtsu, Shuji, Takebe, Hiraku
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1995
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920909/
https://www.ncbi.nlm.nih.gov/pubmed/7559091
http://dx.doi.org/10.1111/j.1349-7006.1995.tb02457.x
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author Miyakoshi, Junji
Yamagishi, Nobuyuki
Ohtsu, Shuji
Takebe, Hiraku
author_facet Miyakoshi, Junji
Yamagishi, Nobuyuki
Ohtsu, Shuji
Takebe, Hiraku
author_sort Miyakoshi, Junji
collection PubMed
description Human osteosarcoma SAOS‐2 cells, which have a deletion in p53 gene, were transfected with plasmid pMSVneop53 containing human p53 cDNA and neomycin‐resistance gene. Three clones (SAOS‐MC10, SAOS‐MC11 and SAOS‐MC43) among 60 clones expressed p53 mRNA. No p53 protein was observed in SAOS‐MC10, while SAOS‐MC11 and SAOS‐MC43 produced p53 protein. The molecular weight of p53 protein in SAOS‐MC43 was lower than that in SAOS‐MC11. SAOS‐MC11 and SAOS‐MC43 were more sensitive and more resistant, respectively, to ionizing radiation than the parental SAOS‐2. We suggest that exogenous p53 protein might be one of the factors determining cellular radiosensitivity.
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spelling pubmed-59209092018-05-11 Changes in Radiation Sensitivity of Human Osteosarcoma Cells after p53 Introduction Miyakoshi, Junji Yamagishi, Nobuyuki Ohtsu, Shuji Takebe, Hiraku Jpn J Cancer Res Rapid Communication Human osteosarcoma SAOS‐2 cells, which have a deletion in p53 gene, were transfected with plasmid pMSVneop53 containing human p53 cDNA and neomycin‐resistance gene. Three clones (SAOS‐MC10, SAOS‐MC11 and SAOS‐MC43) among 60 clones expressed p53 mRNA. No p53 protein was observed in SAOS‐MC10, while SAOS‐MC11 and SAOS‐MC43 produced p53 protein. The molecular weight of p53 protein in SAOS‐MC43 was lower than that in SAOS‐MC11. SAOS‐MC11 and SAOS‐MC43 were more sensitive and more resistant, respectively, to ionizing radiation than the parental SAOS‐2. We suggest that exogenous p53 protein might be one of the factors determining cellular radiosensitivity. Blackwell Publishing Ltd 1995-08 /pmc/articles/PMC5920909/ /pubmed/7559091 http://dx.doi.org/10.1111/j.1349-7006.1995.tb02457.x Text en
spellingShingle Rapid Communication
Miyakoshi, Junji
Yamagishi, Nobuyuki
Ohtsu, Shuji
Takebe, Hiraku
Changes in Radiation Sensitivity of Human Osteosarcoma Cells after p53 Introduction
title Changes in Radiation Sensitivity of Human Osteosarcoma Cells after p53 Introduction
title_full Changes in Radiation Sensitivity of Human Osteosarcoma Cells after p53 Introduction
title_fullStr Changes in Radiation Sensitivity of Human Osteosarcoma Cells after p53 Introduction
title_full_unstemmed Changes in Radiation Sensitivity of Human Osteosarcoma Cells after p53 Introduction
title_short Changes in Radiation Sensitivity of Human Osteosarcoma Cells after p53 Introduction
title_sort changes in radiation sensitivity of human osteosarcoma cells after p53 introduction
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920909/
https://www.ncbi.nlm.nih.gov/pubmed/7559091
http://dx.doi.org/10.1111/j.1349-7006.1995.tb02457.x
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AT ohtsushuji changesinradiationsensitivityofhumanosteosarcomacellsafterp53introduction
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