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High‐level Expression of the CD44 Variant Sharing Exon v10 in Renal Cancer

To examine whether renal cell carcinoma displays altered CD44 expression we performed reverse transcription‐polymerase chain reaction (RT‐PCR) analysis of CD44 in 38 specimens from renal cancer, normal kidney and metastases of 19 patients and 6 renal cancer cell lines. To detect the CD44 variants, w...

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Detalles Bibliográficos
Autores principales: Kan, Masaharu, Aki, Masashi, Akiyama, Kinya, Naruo, Seiichi, Kanayama, Hiro‐omi, Kagawa, Susumu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1995
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920934/
https://www.ncbi.nlm.nih.gov/pubmed/7591962
http://dx.doi.org/10.1111/j.1349-7006.1995.tb03095.x
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author Kan, Masaharu
Aki, Masashi
Akiyama, Kinya
Naruo, Seiichi
Kanayama, Hiro‐omi
Kagawa, Susumu
author_facet Kan, Masaharu
Aki, Masashi
Akiyama, Kinya
Naruo, Seiichi
Kanayama, Hiro‐omi
Kagawa, Susumu
author_sort Kan, Masaharu
collection PubMed
description To examine whether renal cell carcinoma displays altered CD44 expression we performed reverse transcription‐polymerase chain reaction (RT‐PCR) analysis of CD44 in 38 specimens from renal cancer, normal kidney and metastases of 19 patients and 6 renal cancer cell lines. To detect the CD44 variants, we utilized the RT‐PCR Southern blot method. One out of 19 (5.3%) renal cancer specimens expressed a larger molecular weight band than 1 kb by RT‐PCR analysis, in contrast to previous findings in colon and breast cancer. The band patterns in RT‐PCR were different in 14/17 (82.4%) cases between normal kidney and tumors, and a band of about 700 bp was especially marked in 12/17 (70.6%) tumor specimens and 4/6 (66.7%) cell lines. By cloning and sequencing of the 700 bp band, we found that this variant is identical to the CD44 variant sharing only exon v10. Examination by Northern blot analysis has revealed that all tumors express a higher level of CD44 mRNA than paired normal kidneys. These findings suggested that the CD44 variants sharing exon v10 play some role in renal cancer.
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spelling pubmed-59209342018-05-11 High‐level Expression of the CD44 Variant Sharing Exon v10 in Renal Cancer Kan, Masaharu Aki, Masashi Akiyama, Kinya Naruo, Seiichi Kanayama, Hiro‐omi Kagawa, Susumu Jpn J Cancer Res Article To examine whether renal cell carcinoma displays altered CD44 expression we performed reverse transcription‐polymerase chain reaction (RT‐PCR) analysis of CD44 in 38 specimens from renal cancer, normal kidney and metastases of 19 patients and 6 renal cancer cell lines. To detect the CD44 variants, we utilized the RT‐PCR Southern blot method. One out of 19 (5.3%) renal cancer specimens expressed a larger molecular weight band than 1 kb by RT‐PCR analysis, in contrast to previous findings in colon and breast cancer. The band patterns in RT‐PCR were different in 14/17 (82.4%) cases between normal kidney and tumors, and a band of about 700 bp was especially marked in 12/17 (70.6%) tumor specimens and 4/6 (66.7%) cell lines. By cloning and sequencing of the 700 bp band, we found that this variant is identical to the CD44 variant sharing only exon v10. Examination by Northern blot analysis has revealed that all tumors express a higher level of CD44 mRNA than paired normal kidneys. These findings suggested that the CD44 variants sharing exon v10 play some role in renal cancer. Blackwell Publishing Ltd 1995-09 /pmc/articles/PMC5920934/ /pubmed/7591962 http://dx.doi.org/10.1111/j.1349-7006.1995.tb03095.x Text en
spellingShingle Article
Kan, Masaharu
Aki, Masashi
Akiyama, Kinya
Naruo, Seiichi
Kanayama, Hiro‐omi
Kagawa, Susumu
High‐level Expression of the CD44 Variant Sharing Exon v10 in Renal Cancer
title High‐level Expression of the CD44 Variant Sharing Exon v10 in Renal Cancer
title_full High‐level Expression of the CD44 Variant Sharing Exon v10 in Renal Cancer
title_fullStr High‐level Expression of the CD44 Variant Sharing Exon v10 in Renal Cancer
title_full_unstemmed High‐level Expression of the CD44 Variant Sharing Exon v10 in Renal Cancer
title_short High‐level Expression of the CD44 Variant Sharing Exon v10 in Renal Cancer
title_sort high‐level expression of the cd44 variant sharing exon v10 in renal cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920934/
https://www.ncbi.nlm.nih.gov/pubmed/7591962
http://dx.doi.org/10.1111/j.1349-7006.1995.tb03095.x
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