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High‐level Expression of the CD44 Variant Sharing Exon v10 in Renal Cancer
To examine whether renal cell carcinoma displays altered CD44 expression we performed reverse transcription‐polymerase chain reaction (RT‐PCR) analysis of CD44 in 38 specimens from renal cancer, normal kidney and metastases of 19 patients and 6 renal cancer cell lines. To detect the CD44 variants, w...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
1995
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920934/ https://www.ncbi.nlm.nih.gov/pubmed/7591962 http://dx.doi.org/10.1111/j.1349-7006.1995.tb03095.x |
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author | Kan, Masaharu Aki, Masashi Akiyama, Kinya Naruo, Seiichi Kanayama, Hiro‐omi Kagawa, Susumu |
author_facet | Kan, Masaharu Aki, Masashi Akiyama, Kinya Naruo, Seiichi Kanayama, Hiro‐omi Kagawa, Susumu |
author_sort | Kan, Masaharu |
collection | PubMed |
description | To examine whether renal cell carcinoma displays altered CD44 expression we performed reverse transcription‐polymerase chain reaction (RT‐PCR) analysis of CD44 in 38 specimens from renal cancer, normal kidney and metastases of 19 patients and 6 renal cancer cell lines. To detect the CD44 variants, we utilized the RT‐PCR Southern blot method. One out of 19 (5.3%) renal cancer specimens expressed a larger molecular weight band than 1 kb by RT‐PCR analysis, in contrast to previous findings in colon and breast cancer. The band patterns in RT‐PCR were different in 14/17 (82.4%) cases between normal kidney and tumors, and a band of about 700 bp was especially marked in 12/17 (70.6%) tumor specimens and 4/6 (66.7%) cell lines. By cloning and sequencing of the 700 bp band, we found that this variant is identical to the CD44 variant sharing only exon v10. Examination by Northern blot analysis has revealed that all tumors express a higher level of CD44 mRNA than paired normal kidneys. These findings suggested that the CD44 variants sharing exon v10 play some role in renal cancer. |
format | Online Article Text |
id | pubmed-5920934 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1995 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59209342018-05-11 High‐level Expression of the CD44 Variant Sharing Exon v10 in Renal Cancer Kan, Masaharu Aki, Masashi Akiyama, Kinya Naruo, Seiichi Kanayama, Hiro‐omi Kagawa, Susumu Jpn J Cancer Res Article To examine whether renal cell carcinoma displays altered CD44 expression we performed reverse transcription‐polymerase chain reaction (RT‐PCR) analysis of CD44 in 38 specimens from renal cancer, normal kidney and metastases of 19 patients and 6 renal cancer cell lines. To detect the CD44 variants, we utilized the RT‐PCR Southern blot method. One out of 19 (5.3%) renal cancer specimens expressed a larger molecular weight band than 1 kb by RT‐PCR analysis, in contrast to previous findings in colon and breast cancer. The band patterns in RT‐PCR were different in 14/17 (82.4%) cases between normal kidney and tumors, and a band of about 700 bp was especially marked in 12/17 (70.6%) tumor specimens and 4/6 (66.7%) cell lines. By cloning and sequencing of the 700 bp band, we found that this variant is identical to the CD44 variant sharing only exon v10. Examination by Northern blot analysis has revealed that all tumors express a higher level of CD44 mRNA than paired normal kidneys. These findings suggested that the CD44 variants sharing exon v10 play some role in renal cancer. Blackwell Publishing Ltd 1995-09 /pmc/articles/PMC5920934/ /pubmed/7591962 http://dx.doi.org/10.1111/j.1349-7006.1995.tb03095.x Text en |
spellingShingle | Article Kan, Masaharu Aki, Masashi Akiyama, Kinya Naruo, Seiichi Kanayama, Hiro‐omi Kagawa, Susumu High‐level Expression of the CD44 Variant Sharing Exon v10 in Renal Cancer |
title | High‐level Expression of the CD44 Variant Sharing Exon v10 in Renal Cancer |
title_full | High‐level Expression of the CD44 Variant Sharing Exon v10 in Renal Cancer |
title_fullStr | High‐level Expression of the CD44 Variant Sharing Exon v10 in Renal Cancer |
title_full_unstemmed | High‐level Expression of the CD44 Variant Sharing Exon v10 in Renal Cancer |
title_short | High‐level Expression of the CD44 Variant Sharing Exon v10 in Renal Cancer |
title_sort | high‐level expression of the cd44 variant sharing exon v10 in renal cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920934/ https://www.ncbi.nlm.nih.gov/pubmed/7591962 http://dx.doi.org/10.1111/j.1349-7006.1995.tb03095.x |
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