Non‐radioisotopic and Semi‐quantitative Procedure for Terminal Repeat Amplification Protocol

We have used fluorescence‐labeled primers and an auto‐sequencer to detect telomerase activity quickly and easily. The current procedure is superior to the original telomeric repeat amplification protocol in several respects: 1) the result is obtained in real time during electrophoresis, 2) semi‐quan...

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Detalles Bibliográficos
Autores principales: Ohyashiki, Junko H., Ohyashiki, Kazuma, Sano, Tetsuro, Toyama, Keisuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1996
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921098/
https://www.ncbi.nlm.nih.gov/pubmed/8641961
http://dx.doi.org/10.1111/j.1349-7006.1996.tb00225.x
Descripción
Sumario:We have used fluorescence‐labeled primers and an auto‐sequencer to detect telomerase activity quickly and easily. The current procedure is superior to the original telomeric repeat amplification protocol in several respects: 1) the result is obtained in real time during electrophoresis, 2) semi‐quantitative results are possible without using a photo‐capture system, and 3) no radioisotope is needed.

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