Non‐radioisotopic and Semi‐quantitative Procedure for Terminal Repeat Amplification Protocol

We have used fluorescence‐labeled primers and an auto‐sequencer to detect telomerase activity quickly and easily. The current procedure is superior to the original telomeric repeat amplification protocol in several respects: 1) the result is obtained in real time during electrophoresis, 2) semi‐quan...

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Detalles Bibliográficos
Autores principales: Ohyashiki, Junko H., Ohyashiki, Kazuma, Sano, Tetsuro, Toyama, Keisuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1996
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921098/
https://www.ncbi.nlm.nih.gov/pubmed/8641961
http://dx.doi.org/10.1111/j.1349-7006.1996.tb00225.x
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author Ohyashiki, Junko H.
Ohyashiki, Kazuma
Sano, Tetsuro
Toyama, Keisuke
author_facet Ohyashiki, Junko H.
Ohyashiki, Kazuma
Sano, Tetsuro
Toyama, Keisuke
author_sort Ohyashiki, Junko H.
collection PubMed
description We have used fluorescence‐labeled primers and an auto‐sequencer to detect telomerase activity quickly and easily. The current procedure is superior to the original telomeric repeat amplification protocol in several respects: 1) the result is obtained in real time during electrophoresis, 2) semi‐quantitative results are possible without using a photo‐capture system, and 3) no radioisotope is needed.
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spelling pubmed-59210982018-05-11 Non‐radioisotopic and Semi‐quantitative Procedure for Terminal Repeat Amplification Protocol Ohyashiki, Junko H. Ohyashiki, Kazuma Sano, Tetsuro Toyama, Keisuke Jpn J Cancer Res Rapid Communication We have used fluorescence‐labeled primers and an auto‐sequencer to detect telomerase activity quickly and easily. The current procedure is superior to the original telomeric repeat amplification protocol in several respects: 1) the result is obtained in real time during electrophoresis, 2) semi‐quantitative results are possible without using a photo‐capture system, and 3) no radioisotope is needed. Blackwell Publishing Ltd 1996-04 /pmc/articles/PMC5921098/ /pubmed/8641961 http://dx.doi.org/10.1111/j.1349-7006.1996.tb00225.x Text en
spellingShingle Rapid Communication
Ohyashiki, Junko H.
Ohyashiki, Kazuma
Sano, Tetsuro
Toyama, Keisuke
Non‐radioisotopic and Semi‐quantitative Procedure for Terminal Repeat Amplification Protocol
title Non‐radioisotopic and Semi‐quantitative Procedure for Terminal Repeat Amplification Protocol
title_full Non‐radioisotopic and Semi‐quantitative Procedure for Terminal Repeat Amplification Protocol
title_fullStr Non‐radioisotopic and Semi‐quantitative Procedure for Terminal Repeat Amplification Protocol
title_full_unstemmed Non‐radioisotopic and Semi‐quantitative Procedure for Terminal Repeat Amplification Protocol
title_short Non‐radioisotopic and Semi‐quantitative Procedure for Terminal Repeat Amplification Protocol
title_sort non‐radioisotopic and semi‐quantitative procedure for terminal repeat amplification protocol
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921098/
https://www.ncbi.nlm.nih.gov/pubmed/8641961
http://dx.doi.org/10.1111/j.1349-7006.1996.tb00225.x
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