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Correlation between nm23 Protein and Several Cell Adhesion Molecules in Human Gastric Carcinoma

The correlation between nm23 protein (nm23) expression and the expression of several cell adhesion molecules was studied immunohistochemically in 110 resected gastric carcinomas. Formalin‐fixed and paraffin‐embedded samples were serially sectioned and stained with antibodies against nm23, integrin β...

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Detalles Bibliográficos
Autores principales: Ura, Hideki, Denno, Ryuichi, Hirata, Koichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1996
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921117/
https://www.ncbi.nlm.nih.gov/pubmed/8641989
http://dx.doi.org/10.1111/j.1349-7006.1996.tb00253.x
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author Ura, Hideki
Denno, Ryuichi
Hirata, Koichi
author_facet Ura, Hideki
Denno, Ryuichi
Hirata, Koichi
author_sort Ura, Hideki
collection PubMed
description The correlation between nm23 protein (nm23) expression and the expression of several cell adhesion molecules was studied immunohistochemically in 110 resected gastric carcinomas. Formalin‐fixed and paraffin‐embedded samples were serially sectioned and stained with antibodies against nm23, integrin β(1) subfamily members (α(2)β(1), α(3)β(1) and α(4)β(1)), LFA‐1, ICAM‐1, sialyl Lewis(x) (sLe(x)) and CD44h, ‐V3, and ‐V6. Primary carcinomas presenting with either lymph node involvement or liver metastasis expressed significantly reduced levels of nm23 compared to tumors without metastasis. The percent of tumors expressing each adhesion molecule was as follows: α(2)β(1), 27.3%; α(3)β(1), 20.0%; α(4)β(1), 14.5%; LFA‐1, 14.5%; ICAM‐1, 12.7%; sLe(x), 67.3%; CD44h, 55.5%; CD44V3, 20.0%; and CD44V6, 4.5%. Expression of α(2)β(1) integrin and high levels of sLe(x) were significantly correlated with lymph node metastasis, and expression of α(3)β(1) integrin and high levels of sLe(x) were correlated with liver metastasis. Expression of ICAM‐1 was inversely correlated with liver metastasis. Comparing the expression of each cell adhesion molecule with nm23 immunoreactivity, expression of sLe(x) was significantly associated with nm23 expression. Of tumors expressing high levels of sLe(x), 75% showed reduced nm23 expression, compared to 52% of tumors with weak or no sLe(x) expression (P < 0.05). A similar tendency was also observed in the metastasized secondary tumors. These results suggest that reduced nm23 expression may promote the metastatic properties of cancer cells in concert with increased sLe(x) expression.
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spelling pubmed-59211172018-05-11 Correlation between nm23 Protein and Several Cell Adhesion Molecules in Human Gastric Carcinoma Ura, Hideki Denno, Ryuichi Hirata, Koichi Jpn J Cancer Res Article The correlation between nm23 protein (nm23) expression and the expression of several cell adhesion molecules was studied immunohistochemically in 110 resected gastric carcinomas. Formalin‐fixed and paraffin‐embedded samples were serially sectioned and stained with antibodies against nm23, integrin β(1) subfamily members (α(2)β(1), α(3)β(1) and α(4)β(1)), LFA‐1, ICAM‐1, sialyl Lewis(x) (sLe(x)) and CD44h, ‐V3, and ‐V6. Primary carcinomas presenting with either lymph node involvement or liver metastasis expressed significantly reduced levels of nm23 compared to tumors without metastasis. The percent of tumors expressing each adhesion molecule was as follows: α(2)β(1), 27.3%; α(3)β(1), 20.0%; α(4)β(1), 14.5%; LFA‐1, 14.5%; ICAM‐1, 12.7%; sLe(x), 67.3%; CD44h, 55.5%; CD44V3, 20.0%; and CD44V6, 4.5%. Expression of α(2)β(1) integrin and high levels of sLe(x) were significantly correlated with lymph node metastasis, and expression of α(3)β(1) integrin and high levels of sLe(x) were correlated with liver metastasis. Expression of ICAM‐1 was inversely correlated with liver metastasis. Comparing the expression of each cell adhesion molecule with nm23 immunoreactivity, expression of sLe(x) was significantly associated with nm23 expression. Of tumors expressing high levels of sLe(x), 75% showed reduced nm23 expression, compared to 52% of tumors with weak or no sLe(x) expression (P < 0.05). A similar tendency was also observed in the metastasized secondary tumors. These results suggest that reduced nm23 expression may promote the metastatic properties of cancer cells in concert with increased sLe(x) expression. Blackwell Publishing Ltd 1996-05 /pmc/articles/PMC5921117/ /pubmed/8641989 http://dx.doi.org/10.1111/j.1349-7006.1996.tb00253.x Text en
spellingShingle Article
Ura, Hideki
Denno, Ryuichi
Hirata, Koichi
Correlation between nm23 Protein and Several Cell Adhesion Molecules in Human Gastric Carcinoma
title Correlation between nm23 Protein and Several Cell Adhesion Molecules in Human Gastric Carcinoma
title_full Correlation between nm23 Protein and Several Cell Adhesion Molecules in Human Gastric Carcinoma
title_fullStr Correlation between nm23 Protein and Several Cell Adhesion Molecules in Human Gastric Carcinoma
title_full_unstemmed Correlation between nm23 Protein and Several Cell Adhesion Molecules in Human Gastric Carcinoma
title_short Correlation between nm23 Protein and Several Cell Adhesion Molecules in Human Gastric Carcinoma
title_sort correlation between nm23 protein and several cell adhesion molecules in human gastric carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921117/
https://www.ncbi.nlm.nih.gov/pubmed/8641989
http://dx.doi.org/10.1111/j.1349-7006.1996.tb00253.x
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