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Tumor Necrosis Factor‐α Gene Transfer Augments Anti‐Fas Antibody‐mediated Apoptosis in Human Glioma Cells

To effectively induce apoptosis in human glioma cells, we tried to transfer the tumor necrosis factor (TNF)‐α gene into glioma cells to produce TNF‐α locally in these cells. The stable transfectants of three gliorna cells (U251‐SP, U251‐MG, and T98G) were resistant to exogenous TNF‐α, but their cell...

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Detalles Bibliográficos
Autores principales: Mizuno, Masaaki, Yoshida, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1996
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921120/
https://www.ncbi.nlm.nih.gov/pubmed/8641993
http://dx.doi.org/10.1111/j.1349-7006.1996.tb00257.x
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author Mizuno, Masaaki
Yoshida, Jun
author_facet Mizuno, Masaaki
Yoshida, Jun
author_sort Mizuno, Masaaki
collection PubMed
description To effectively induce apoptosis in human glioma cells, we tried to transfer the tumor necrosis factor (TNF)‐α gene into glioma cells to produce TNF‐α locally in these cells. The stable transfectants of three gliorna cells (U251‐SP, U251‐MG, and T98G) were resistant to exogenous TNF‐α, but their cell surface expression of the Fas antigen was dramatically enhanced by about 10 to 100‐fold as compared with untransfected glioma cells exposed to exogenous TNF‐α. The Fas antigen is a transmemhrane cytokine receptor protein of the nerve growth factor/TNF receptor superfamily. Although the untransfected glioma cells tested were resistant to anti‐Fas antibody‐mediated apoptosis, the TNF‐α gene‐transfected glioma cells exhibited high susceptibility to anti‐Fas antibody‐mediated apoptosis. Thus, TNF‐α gene transfer combined with anti‐Fas antibodies may be useful for the treatment of malignant glioma.
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spelling pubmed-59211202018-05-11 Tumor Necrosis Factor‐α Gene Transfer Augments Anti‐Fas Antibody‐mediated Apoptosis in Human Glioma Cells Mizuno, Masaaki Yoshida, Jun Jpn J Cancer Res Article To effectively induce apoptosis in human glioma cells, we tried to transfer the tumor necrosis factor (TNF)‐α gene into glioma cells to produce TNF‐α locally in these cells. The stable transfectants of three gliorna cells (U251‐SP, U251‐MG, and T98G) were resistant to exogenous TNF‐α, but their cell surface expression of the Fas antigen was dramatically enhanced by about 10 to 100‐fold as compared with untransfected glioma cells exposed to exogenous TNF‐α. The Fas antigen is a transmemhrane cytokine receptor protein of the nerve growth factor/TNF receptor superfamily. Although the untransfected glioma cells tested were resistant to anti‐Fas antibody‐mediated apoptosis, the TNF‐α gene‐transfected glioma cells exhibited high susceptibility to anti‐Fas antibody‐mediated apoptosis. Thus, TNF‐α gene transfer combined with anti‐Fas antibodies may be useful for the treatment of malignant glioma. Blackwell Publishing Ltd 1996-05 /pmc/articles/PMC5921120/ /pubmed/8641993 http://dx.doi.org/10.1111/j.1349-7006.1996.tb00257.x Text en
spellingShingle Article
Mizuno, Masaaki
Yoshida, Jun
Tumor Necrosis Factor‐α Gene Transfer Augments Anti‐Fas Antibody‐mediated Apoptosis in Human Glioma Cells
title Tumor Necrosis Factor‐α Gene Transfer Augments Anti‐Fas Antibody‐mediated Apoptosis in Human Glioma Cells
title_full Tumor Necrosis Factor‐α Gene Transfer Augments Anti‐Fas Antibody‐mediated Apoptosis in Human Glioma Cells
title_fullStr Tumor Necrosis Factor‐α Gene Transfer Augments Anti‐Fas Antibody‐mediated Apoptosis in Human Glioma Cells
title_full_unstemmed Tumor Necrosis Factor‐α Gene Transfer Augments Anti‐Fas Antibody‐mediated Apoptosis in Human Glioma Cells
title_short Tumor Necrosis Factor‐α Gene Transfer Augments Anti‐Fas Antibody‐mediated Apoptosis in Human Glioma Cells
title_sort tumor necrosis factor‐α gene transfer augments anti‐fas antibody‐mediated apoptosis in human glioma cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921120/
https://www.ncbi.nlm.nih.gov/pubmed/8641993
http://dx.doi.org/10.1111/j.1349-7006.1996.tb00257.x
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