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Tumor Necrosis Factor‐α Gene Transfer Augments Anti‐Fas Antibody‐mediated Apoptosis in Human Glioma Cells
To effectively induce apoptosis in human glioma cells, we tried to transfer the tumor necrosis factor (TNF)‐α gene into glioma cells to produce TNF‐α locally in these cells. The stable transfectants of three gliorna cells (U251‐SP, U251‐MG, and T98G) were resistant to exogenous TNF‐α, but their cell...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
1996
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921120/ https://www.ncbi.nlm.nih.gov/pubmed/8641993 http://dx.doi.org/10.1111/j.1349-7006.1996.tb00257.x |
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author | Mizuno, Masaaki Yoshida, Jun |
author_facet | Mizuno, Masaaki Yoshida, Jun |
author_sort | Mizuno, Masaaki |
collection | PubMed |
description | To effectively induce apoptosis in human glioma cells, we tried to transfer the tumor necrosis factor (TNF)‐α gene into glioma cells to produce TNF‐α locally in these cells. The stable transfectants of three gliorna cells (U251‐SP, U251‐MG, and T98G) were resistant to exogenous TNF‐α, but their cell surface expression of the Fas antigen was dramatically enhanced by about 10 to 100‐fold as compared with untransfected glioma cells exposed to exogenous TNF‐α. The Fas antigen is a transmemhrane cytokine receptor protein of the nerve growth factor/TNF receptor superfamily. Although the untransfected glioma cells tested were resistant to anti‐Fas antibody‐mediated apoptosis, the TNF‐α gene‐transfected glioma cells exhibited high susceptibility to anti‐Fas antibody‐mediated apoptosis. Thus, TNF‐α gene transfer combined with anti‐Fas antibodies may be useful for the treatment of malignant glioma. |
format | Online Article Text |
id | pubmed-5921120 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1996 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59211202018-05-11 Tumor Necrosis Factor‐α Gene Transfer Augments Anti‐Fas Antibody‐mediated Apoptosis in Human Glioma Cells Mizuno, Masaaki Yoshida, Jun Jpn J Cancer Res Article To effectively induce apoptosis in human glioma cells, we tried to transfer the tumor necrosis factor (TNF)‐α gene into glioma cells to produce TNF‐α locally in these cells. The stable transfectants of three gliorna cells (U251‐SP, U251‐MG, and T98G) were resistant to exogenous TNF‐α, but their cell surface expression of the Fas antigen was dramatically enhanced by about 10 to 100‐fold as compared with untransfected glioma cells exposed to exogenous TNF‐α. The Fas antigen is a transmemhrane cytokine receptor protein of the nerve growth factor/TNF receptor superfamily. Although the untransfected glioma cells tested were resistant to anti‐Fas antibody‐mediated apoptosis, the TNF‐α gene‐transfected glioma cells exhibited high susceptibility to anti‐Fas antibody‐mediated apoptosis. Thus, TNF‐α gene transfer combined with anti‐Fas antibodies may be useful for the treatment of malignant glioma. Blackwell Publishing Ltd 1996-05 /pmc/articles/PMC5921120/ /pubmed/8641993 http://dx.doi.org/10.1111/j.1349-7006.1996.tb00257.x Text en |
spellingShingle | Article Mizuno, Masaaki Yoshida, Jun Tumor Necrosis Factor‐α Gene Transfer Augments Anti‐Fas Antibody‐mediated Apoptosis in Human Glioma Cells |
title | Tumor Necrosis Factor‐α Gene Transfer Augments Anti‐Fas Antibody‐mediated Apoptosis in Human Glioma Cells |
title_full | Tumor Necrosis Factor‐α Gene Transfer Augments Anti‐Fas Antibody‐mediated Apoptosis in Human Glioma Cells |
title_fullStr | Tumor Necrosis Factor‐α Gene Transfer Augments Anti‐Fas Antibody‐mediated Apoptosis in Human Glioma Cells |
title_full_unstemmed | Tumor Necrosis Factor‐α Gene Transfer Augments Anti‐Fas Antibody‐mediated Apoptosis in Human Glioma Cells |
title_short | Tumor Necrosis Factor‐α Gene Transfer Augments Anti‐Fas Antibody‐mediated Apoptosis in Human Glioma Cells |
title_sort | tumor necrosis factor‐α gene transfer augments anti‐fas antibody‐mediated apoptosis in human glioma cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921120/ https://www.ncbi.nlm.nih.gov/pubmed/8641993 http://dx.doi.org/10.1111/j.1349-7006.1996.tb00257.x |
work_keys_str_mv | AT mizunomasaaki tumornecrosisfactoragenetransferaugmentsantifasantibodymediatedapoptosisinhumangliomacells AT yoshidajun tumornecrosisfactoragenetransferaugmentsantifasantibodymediatedapoptosisinhumangliomacells |