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Helicobacter pylori Extracts Exhibit Nicotinamide Adenine Dinucleotide‐derived Adenylation but Not Mono(adenosine 5‐diphosphate‐ribosyOation of DNA Ligase

The issue of toxins produced by Helicobacter pylori (H. pylori) urgently requires clarification given that the bacterium causes gastric epithelial cell damage which may lead to precancerous and cancerous changes. During an investigation of the possibility of mono(adenosine 5′‐diphosphate (ADP)‐ribos...

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Autores principales: Nozaki, Tadashige, Masutani, Mitsuko, Noda, Takeshi, Saito, Daizo, Sugiyama, Toshiro, Takato, Tsuyoshi, Wakabayashi, Keiji, Nakagama, Hitoshi, Sugimura, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1997
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921279/
https://www.ncbi.nlm.nih.gov/pubmed/9414650
http://dx.doi.org/10.1111/j.1349-7006.1997.tb00308.x
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author Nozaki, Tadashige
Masutani, Mitsuko
Noda, Takeshi
Saito, Daizo
Sugiyama, Toshiro
Takato, Tsuyoshi
Wakabayashi, Keiji
Nakagama, Hitoshi
Sugimura, Takashi
author_facet Nozaki, Tadashige
Masutani, Mitsuko
Noda, Takeshi
Saito, Daizo
Sugiyama, Toshiro
Takato, Tsuyoshi
Wakabayashi, Keiji
Nakagama, Hitoshi
Sugimura, Takashi
author_sort Nozaki, Tadashige
collection PubMed
description The issue of toxins produced by Helicobacter pylori (H. pylori) urgently requires clarification given that the bacterium causes gastric epithelial cell damage which may lead to precancerous and cancerous changes. During an investigation of the possibility of mono(adenosine 5′‐diphosphate (ADP)‐ribosyl)‐ation by H. pylori products, as observed for other bacterial toxins, we found that radioactivity of [adenylate‐(32)P]nicotinamide adenine dinucleotide (NAD) is incorporated into an H. pylori protein of 80 kDa after incubation with crude bacterial extract. In contrast, [carbonyl‐(14)C]NAD did not show any radioactivity incorporation. Unexpectedly, treatment of the modified protein with 0.1 N HC1, but not 0.1 JV NaOH, released the AMP moiety. Such chemical properties are characteristic of bacterial DNA ligase‐AMP complexes. We found that an antibody raised against Escherichia coli DNA ligase [EC 6.5.1.2] immunoprecipitated the modified 80 kDa protein. Our results indicate that incorporation of radioactivity derived from NAD into the 80 kDa protein was due to adenylation, but not mono‐(ADP‐ribosyl) ation, of the DNA ligase of H. pylori.
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spelling pubmed-59212792018-05-11 Helicobacter pylori Extracts Exhibit Nicotinamide Adenine Dinucleotide‐derived Adenylation but Not Mono(adenosine 5‐diphosphate‐ribosyOation of DNA Ligase Nozaki, Tadashige Masutani, Mitsuko Noda, Takeshi Saito, Daizo Sugiyama, Toshiro Takato, Tsuyoshi Wakabayashi, Keiji Nakagama, Hitoshi Sugimura, Takashi Jpn J Cancer Res Article The issue of toxins produced by Helicobacter pylori (H. pylori) urgently requires clarification given that the bacterium causes gastric epithelial cell damage which may lead to precancerous and cancerous changes. During an investigation of the possibility of mono(adenosine 5′‐diphosphate (ADP)‐ribosyl)‐ation by H. pylori products, as observed for other bacterial toxins, we found that radioactivity of [adenylate‐(32)P]nicotinamide adenine dinucleotide (NAD) is incorporated into an H. pylori protein of 80 kDa after incubation with crude bacterial extract. In contrast, [carbonyl‐(14)C]NAD did not show any radioactivity incorporation. Unexpectedly, treatment of the modified protein with 0.1 N HC1, but not 0.1 JV NaOH, released the AMP moiety. Such chemical properties are characteristic of bacterial DNA ligase‐AMP complexes. We found that an antibody raised against Escherichia coli DNA ligase [EC 6.5.1.2] immunoprecipitated the modified 80 kDa protein. Our results indicate that incorporation of radioactivity derived from NAD into the 80 kDa protein was due to adenylation, but not mono‐(ADP‐ribosyl) ation, of the DNA ligase of H. pylori. Blackwell Publishing Ltd 1997-10 /pmc/articles/PMC5921279/ /pubmed/9414650 http://dx.doi.org/10.1111/j.1349-7006.1997.tb00308.x Text en
spellingShingle Article
Nozaki, Tadashige
Masutani, Mitsuko
Noda, Takeshi
Saito, Daizo
Sugiyama, Toshiro
Takato, Tsuyoshi
Wakabayashi, Keiji
Nakagama, Hitoshi
Sugimura, Takashi
Helicobacter pylori Extracts Exhibit Nicotinamide Adenine Dinucleotide‐derived Adenylation but Not Mono(adenosine 5‐diphosphate‐ribosyOation of DNA Ligase
title Helicobacter pylori Extracts Exhibit Nicotinamide Adenine Dinucleotide‐derived Adenylation but Not Mono(adenosine 5‐diphosphate‐ribosyOation of DNA Ligase
title_full Helicobacter pylori Extracts Exhibit Nicotinamide Adenine Dinucleotide‐derived Adenylation but Not Mono(adenosine 5‐diphosphate‐ribosyOation of DNA Ligase
title_fullStr Helicobacter pylori Extracts Exhibit Nicotinamide Adenine Dinucleotide‐derived Adenylation but Not Mono(adenosine 5‐diphosphate‐ribosyOation of DNA Ligase
title_full_unstemmed Helicobacter pylori Extracts Exhibit Nicotinamide Adenine Dinucleotide‐derived Adenylation but Not Mono(adenosine 5‐diphosphate‐ribosyOation of DNA Ligase
title_short Helicobacter pylori Extracts Exhibit Nicotinamide Adenine Dinucleotide‐derived Adenylation but Not Mono(adenosine 5‐diphosphate‐ribosyOation of DNA Ligase
title_sort helicobacter pylori extracts exhibit nicotinamide adenine dinucleotide‐derived adenylation but not mono(adenosine 5‐diphosphate‐ribosyoation of dna ligase
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921279/
https://www.ncbi.nlm.nih.gov/pubmed/9414650
http://dx.doi.org/10.1111/j.1349-7006.1997.tb00308.x
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