Cargando…
Increased DNA Methyltransferase Expression Is Associated with an Early Stage of Human Hepatocarcinogenesis
The present study was designed to determine whether changes in DNA methyltransferase (DNA MTase) expression are involved in hepatocarcinogenesis. We examined DNA MTase expression in normal liver tissue (with no remarkable histological findings), liver tissue showing chronic hepatitis or cirrhosis, w...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
1997
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921336/ https://www.ncbi.nlm.nih.gov/pubmed/9473734 http://dx.doi.org/10.1111/j.1349-7006.1997.tb00345.x |
_version_ | 1783317987383574528 |
---|---|
author | Sun, Lin Hui, Ai‐Min Kanai, Yae Sakamoto, Michiie Hirohashi, Setsuo |
author_facet | Sun, Lin Hui, Ai‐Min Kanai, Yae Sakamoto, Michiie Hirohashi, Setsuo |
author_sort | Sun, Lin |
collection | PubMed |
description | The present study was designed to determine whether changes in DNA methyltransferase (DNA MTase) expression are involved in hepatocarcinogenesis. We examined DNA MTase expression in normal liver tissue (with no remarkable histological findings), liver tissue showing chronic hepatitis or cirrhosis, which are generally thought to be precancerous conditions, and hepatocellular carcinomas (HCCs) using the reverse‐transcriptase polymerase chain reaction assay. DNA MTase mRNA levels were significantly higher in liver tissue showing chronic hepatitis and cirrhosis (DNA MTase mRNA/β‐actin mRNA ratio=0.30±0.22, n=24, P<0.01) than in normal liver tissue either from patients with liver metastatic lesions of colonic cancer (0.14±0.05, n= 6) or from patients with HCCs (0.16±0.07, n= 3). DNA MTase mRNA levels were even higher in HCC tissue (0.34±0.18, n=29). These results suggest that increased DNA MTase expression may be an early event during hepatocar‐cinogenesis. DNA MTase is a potential target for HCC preventive therapy. |
format | Online Article Text |
id | pubmed-5921336 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1997 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59213362018-05-11 Increased DNA Methyltransferase Expression Is Associated with an Early Stage of Human Hepatocarcinogenesis Sun, Lin Hui, Ai‐Min Kanai, Yae Sakamoto, Michiie Hirohashi, Setsuo Jpn J Cancer Res Article The present study was designed to determine whether changes in DNA methyltransferase (DNA MTase) expression are involved in hepatocarcinogenesis. We examined DNA MTase expression in normal liver tissue (with no remarkable histological findings), liver tissue showing chronic hepatitis or cirrhosis, which are generally thought to be precancerous conditions, and hepatocellular carcinomas (HCCs) using the reverse‐transcriptase polymerase chain reaction assay. DNA MTase mRNA levels were significantly higher in liver tissue showing chronic hepatitis and cirrhosis (DNA MTase mRNA/β‐actin mRNA ratio=0.30±0.22, n=24, P<0.01) than in normal liver tissue either from patients with liver metastatic lesions of colonic cancer (0.14±0.05, n= 6) or from patients with HCCs (0.16±0.07, n= 3). DNA MTase mRNA levels were even higher in HCC tissue (0.34±0.18, n=29). These results suggest that increased DNA MTase expression may be an early event during hepatocar‐cinogenesis. DNA MTase is a potential target for HCC preventive therapy. Blackwell Publishing Ltd 1997-12 /pmc/articles/PMC5921336/ /pubmed/9473734 http://dx.doi.org/10.1111/j.1349-7006.1997.tb00345.x Text en |
spellingShingle | Article Sun, Lin Hui, Ai‐Min Kanai, Yae Sakamoto, Michiie Hirohashi, Setsuo Increased DNA Methyltransferase Expression Is Associated with an Early Stage of Human Hepatocarcinogenesis |
title | Increased DNA Methyltransferase Expression Is Associated with an Early Stage of Human Hepatocarcinogenesis |
title_full | Increased DNA Methyltransferase Expression Is Associated with an Early Stage of Human Hepatocarcinogenesis |
title_fullStr | Increased DNA Methyltransferase Expression Is Associated with an Early Stage of Human Hepatocarcinogenesis |
title_full_unstemmed | Increased DNA Methyltransferase Expression Is Associated with an Early Stage of Human Hepatocarcinogenesis |
title_short | Increased DNA Methyltransferase Expression Is Associated with an Early Stage of Human Hepatocarcinogenesis |
title_sort | increased dna methyltransferase expression is associated with an early stage of human hepatocarcinogenesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921336/ https://www.ncbi.nlm.nih.gov/pubmed/9473734 http://dx.doi.org/10.1111/j.1349-7006.1997.tb00345.x |
work_keys_str_mv | AT sunlin increaseddnamethyltransferaseexpressionisassociatedwithanearlystageofhumanhepatocarcinogenesis AT huiaimin increaseddnamethyltransferaseexpressionisassociatedwithanearlystageofhumanhepatocarcinogenesis AT kanaiyae increaseddnamethyltransferaseexpressionisassociatedwithanearlystageofhumanhepatocarcinogenesis AT sakamotomichiie increaseddnamethyltransferaseexpressionisassociatedwithanearlystageofhumanhepatocarcinogenesis AT hirohashisetsuo increaseddnamethyltransferaseexpressionisassociatedwithanearlystageofhumanhepatocarcinogenesis |