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Gene Mutation Analysis and Quantitation of DNA Topoisomerase I in Previously Untreated Non‐small Cell Lung Carcinomas

To elucidate whether gene alterations of topoisomerase I (topo I) exist in untreated non‐small cell lung carcinomas (NSCLC), polymerase chain reaction‐single strand conformation polymorphism analysis was performed in forty‐four NSCLC tissue samples. Gene alterations of topo I were sought in three re...

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Autores principales: Takatani, Hiroshi, Oka, Mikio, Fukuda, Minoru, Narasaki, Fumihiko, Nakano, Reiji, Ikeda, Koki, Terashi, Kenji, Kinoshita, Akitoshi, Soda, Hiroshi, Kanda, Tetsuro, Schneider, Erasmus, Kohno, Shigeru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1997
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921358/
https://www.ncbi.nlm.nih.gov/pubmed/9119744
http://dx.doi.org/10.1111/j.1349-7006.1997.tb00361.x
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author Takatani, Hiroshi
Oka, Mikio
Fukuda, Minoru
Narasaki, Fumihiko
Nakano, Reiji
Ikeda, Koki
Terashi, Kenji
Kinoshita, Akitoshi
Soda, Hiroshi
Kanda, Tetsuro
Schneider, Erasmus
Kohno, Shigeru
author_facet Takatani, Hiroshi
Oka, Mikio
Fukuda, Minoru
Narasaki, Fumihiko
Nakano, Reiji
Ikeda, Koki
Terashi, Kenji
Kinoshita, Akitoshi
Soda, Hiroshi
Kanda, Tetsuro
Schneider, Erasmus
Kohno, Shigeru
author_sort Takatani, Hiroshi
collection PubMed
description To elucidate whether gene alterations of topoisomerase I (topo I) exist in untreated non‐small cell lung carcinomas (NSCLC), polymerase chain reaction‐single strand conformation polymorphism analysis was performed in forty‐four NSCLC tissue samples. Gene alterations of topo I were sought in three regions, near codons 361 and 363, 533, and 722 and 729, where point mutations have been found in resistant tumor cell lines selected by chronic camptothecin exposure. In addition, nuclear topo I contents were determined by immunoblotting. No mobility shifts were observed compared to the pattern observed in a normal control at any of the three regions in any sample, whereas topo I levels showed an approximately 12‐fold variation. The variation is remarkably large compared to those seen in previous in vitro and in vivo studies. The results suggest that mutations of topo I may not contribute to intrinsic resistance of NSCLC to camptothecins, but low topo I levels may account, at least in part, for the resistance.
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spelling pubmed-59213582018-05-11 Gene Mutation Analysis and Quantitation of DNA Topoisomerase I in Previously Untreated Non‐small Cell Lung Carcinomas Takatani, Hiroshi Oka, Mikio Fukuda, Minoru Narasaki, Fumihiko Nakano, Reiji Ikeda, Koki Terashi, Kenji Kinoshita, Akitoshi Soda, Hiroshi Kanda, Tetsuro Schneider, Erasmus Kohno, Shigeru Jpn J Cancer Res Article To elucidate whether gene alterations of topoisomerase I (topo I) exist in untreated non‐small cell lung carcinomas (NSCLC), polymerase chain reaction‐single strand conformation polymorphism analysis was performed in forty‐four NSCLC tissue samples. Gene alterations of topo I were sought in three regions, near codons 361 and 363, 533, and 722 and 729, where point mutations have been found in resistant tumor cell lines selected by chronic camptothecin exposure. In addition, nuclear topo I contents were determined by immunoblotting. No mobility shifts were observed compared to the pattern observed in a normal control at any of the three regions in any sample, whereas topo I levels showed an approximately 12‐fold variation. The variation is remarkably large compared to those seen in previous in vitro and in vivo studies. The results suggest that mutations of topo I may not contribute to intrinsic resistance of NSCLC to camptothecins, but low topo I levels may account, at least in part, for the resistance. Blackwell Publishing Ltd 1997-02 /pmc/articles/PMC5921358/ /pubmed/9119744 http://dx.doi.org/10.1111/j.1349-7006.1997.tb00361.x Text en
spellingShingle Article
Takatani, Hiroshi
Oka, Mikio
Fukuda, Minoru
Narasaki, Fumihiko
Nakano, Reiji
Ikeda, Koki
Terashi, Kenji
Kinoshita, Akitoshi
Soda, Hiroshi
Kanda, Tetsuro
Schneider, Erasmus
Kohno, Shigeru
Gene Mutation Analysis and Quantitation of DNA Topoisomerase I in Previously Untreated Non‐small Cell Lung Carcinomas
title Gene Mutation Analysis and Quantitation of DNA Topoisomerase I in Previously Untreated Non‐small Cell Lung Carcinomas
title_full Gene Mutation Analysis and Quantitation of DNA Topoisomerase I in Previously Untreated Non‐small Cell Lung Carcinomas
title_fullStr Gene Mutation Analysis and Quantitation of DNA Topoisomerase I in Previously Untreated Non‐small Cell Lung Carcinomas
title_full_unstemmed Gene Mutation Analysis and Quantitation of DNA Topoisomerase I in Previously Untreated Non‐small Cell Lung Carcinomas
title_short Gene Mutation Analysis and Quantitation of DNA Topoisomerase I in Previously Untreated Non‐small Cell Lung Carcinomas
title_sort gene mutation analysis and quantitation of dna topoisomerase i in previously untreated non‐small cell lung carcinomas
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921358/
https://www.ncbi.nlm.nih.gov/pubmed/9119744
http://dx.doi.org/10.1111/j.1349-7006.1997.tb00361.x
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