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Identification of interneurons required for the aversive response of Caenorhabditis elegans to graphene oxide

BACKGROUND: So far, how the animals evade the environmental nanomaterials is still largely unclear. In this study, we employed in vivo assay system of Caenorhabditis elegans to investigate the aversive behavior of nematodes to graphene oxide (GO) and the underlying neuronal basis. RESULTS: In this a...

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Autores principales: Xiao, Guosheng, Chen, He, Krasteva, Natalia, Liu, Qizhan, Wang, Dayong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921546/
https://www.ncbi.nlm.nih.gov/pubmed/29703212
http://dx.doi.org/10.1186/s12951-018-0373-y
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author Xiao, Guosheng
Chen, He
Krasteva, Natalia
Liu, Qizhan
Wang, Dayong
author_facet Xiao, Guosheng
Chen, He
Krasteva, Natalia
Liu, Qizhan
Wang, Dayong
author_sort Xiao, Guosheng
collection PubMed
description BACKGROUND: So far, how the animals evade the environmental nanomaterials is still largely unclear. In this study, we employed in vivo assay system of Caenorhabditis elegans to investigate the aversive behavior of nematodes to graphene oxide (GO) and the underlying neuronal basis. RESULTS: In this assay model, we detected the significant aversive behavior of nematodes to GO at concentrations more than 50 mg/L. Loss-of-function mutation of nlg-1 encoding a neuroligin with the function in connecting pre- and post-synaptic neurons suppressed the aversive behavior of nematodes to GO. Moreover, based on the neuron-specific activity assay, we found that the NLG-1 activity in AIY or AIB interneurons was required for the regulation of aversive behavior to GO. The neuron-specific activities of NLG-1 in AIY or AIB interneurons were also required for the regulation of GO toxicity. CONCLUSIONS: Using nlg-1 mutant as a genetic tool, we identified the AIY and AIB interneurons required for the regulation of aversive behavior to GO. Our results provide an important neuronal basis for the aversive response of animals to environmental nanomaterials. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12951-018-0373-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-59215462018-05-01 Identification of interneurons required for the aversive response of Caenorhabditis elegans to graphene oxide Xiao, Guosheng Chen, He Krasteva, Natalia Liu, Qizhan Wang, Dayong J Nanobiotechnology Research BACKGROUND: So far, how the animals evade the environmental nanomaterials is still largely unclear. In this study, we employed in vivo assay system of Caenorhabditis elegans to investigate the aversive behavior of nematodes to graphene oxide (GO) and the underlying neuronal basis. RESULTS: In this assay model, we detected the significant aversive behavior of nematodes to GO at concentrations more than 50 mg/L. Loss-of-function mutation of nlg-1 encoding a neuroligin with the function in connecting pre- and post-synaptic neurons suppressed the aversive behavior of nematodes to GO. Moreover, based on the neuron-specific activity assay, we found that the NLG-1 activity in AIY or AIB interneurons was required for the regulation of aversive behavior to GO. The neuron-specific activities of NLG-1 in AIY or AIB interneurons were also required for the regulation of GO toxicity. CONCLUSIONS: Using nlg-1 mutant as a genetic tool, we identified the AIY and AIB interneurons required for the regulation of aversive behavior to GO. Our results provide an important neuronal basis for the aversive response of animals to environmental nanomaterials. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12951-018-0373-y) contains supplementary material, which is available to authorized users. BioMed Central 2018-04-27 /pmc/articles/PMC5921546/ /pubmed/29703212 http://dx.doi.org/10.1186/s12951-018-0373-y Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Xiao, Guosheng
Chen, He
Krasteva, Natalia
Liu, Qizhan
Wang, Dayong
Identification of interneurons required for the aversive response of Caenorhabditis elegans to graphene oxide
title Identification of interneurons required for the aversive response of Caenorhabditis elegans to graphene oxide
title_full Identification of interneurons required for the aversive response of Caenorhabditis elegans to graphene oxide
title_fullStr Identification of interneurons required for the aversive response of Caenorhabditis elegans to graphene oxide
title_full_unstemmed Identification of interneurons required for the aversive response of Caenorhabditis elegans to graphene oxide
title_short Identification of interneurons required for the aversive response of Caenorhabditis elegans to graphene oxide
title_sort identification of interneurons required for the aversive response of caenorhabditis elegans to graphene oxide
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921546/
https://www.ncbi.nlm.nih.gov/pubmed/29703212
http://dx.doi.org/10.1186/s12951-018-0373-y
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