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Involvement in surface antigen expression by a moonlighting FG-repeat nucleoporin in trypanosomes

Components of the nuclear periphery coordinate a multitude of activities, including macromolecular transport, cell-cycle progression, and chromatin organization. Nuclear pore complexes (NPCs) mediate nucleocytoplasmic transport, mRNA processing, and transcriptional regulation, and NPC components can...

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Autores principales: Holden, Jennifer M., Koreny, Ludek, Obado, Samson, Ratushny, Alexander V., Chen, Wei-Ming, Bart, Jean-Mathieu, Navarro, Miguel, Chait, Brian T., Aitchison, John D., Rout, Michael P., Field, Mark C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921576/
https://www.ncbi.nlm.nih.gov/pubmed/29496964
http://dx.doi.org/10.1091/mbc.E17-06-0430
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author Holden, Jennifer M.
Koreny, Ludek
Obado, Samson
Ratushny, Alexander V.
Chen, Wei-Ming
Bart, Jean-Mathieu
Navarro, Miguel
Chait, Brian T.
Aitchison, John D.
Rout, Michael P.
Field, Mark C.
author_facet Holden, Jennifer M.
Koreny, Ludek
Obado, Samson
Ratushny, Alexander V.
Chen, Wei-Ming
Bart, Jean-Mathieu
Navarro, Miguel
Chait, Brian T.
Aitchison, John D.
Rout, Michael P.
Field, Mark C.
author_sort Holden, Jennifer M.
collection PubMed
description Components of the nuclear periphery coordinate a multitude of activities, including macromolecular transport, cell-cycle progression, and chromatin organization. Nuclear pore complexes (NPCs) mediate nucleocytoplasmic transport, mRNA processing, and transcriptional regulation, and NPC components can define regions of high transcriptional activity in some organisms at the nuclear periphery and nucleoplasm. Lineage-specific features underpin several core nuclear functions and in trypanosomatids, which branched very early from other eukaryotes, unique protein components constitute the lamina, kinetochores, and parts of the NPCs. Here we describe a phenylalanine-glycine (FG)-repeat nucleoporin, TbNup53b, that has dual localizations within the nucleoplasm and NPC. In addition to association with nucleoporins, TbNup53b interacts with a known trans-splicing component, TSR1, and has a role in controlling expression of surface proteins including the nucleolar periphery-located, procyclin genes. Significantly, while several nucleoporins are implicated in intranuclear transcriptional regulation in metazoa, TbNup53b appears orthologous to components of the yeast/human Nup49/Nup58 complex, for which no transcriptional functions are known. These data suggest that FG-Nups are frequently co-opted to transcriptional functions during evolution and extend the presence of FG-repeat nucleoporin control of gene expression to trypanosomes, suggesting that this is a widespread and ancient eukaryotic feature, as well as underscoring once more flexibility within nucleoporin function.
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spelling pubmed-59215762018-07-16 Involvement in surface antigen expression by a moonlighting FG-repeat nucleoporin in trypanosomes Holden, Jennifer M. Koreny, Ludek Obado, Samson Ratushny, Alexander V. Chen, Wei-Ming Bart, Jean-Mathieu Navarro, Miguel Chait, Brian T. Aitchison, John D. Rout, Michael P. Field, Mark C. Mol Biol Cell Articles Components of the nuclear periphery coordinate a multitude of activities, including macromolecular transport, cell-cycle progression, and chromatin organization. Nuclear pore complexes (NPCs) mediate nucleocytoplasmic transport, mRNA processing, and transcriptional regulation, and NPC components can define regions of high transcriptional activity in some organisms at the nuclear periphery and nucleoplasm. Lineage-specific features underpin several core nuclear functions and in trypanosomatids, which branched very early from other eukaryotes, unique protein components constitute the lamina, kinetochores, and parts of the NPCs. Here we describe a phenylalanine-glycine (FG)-repeat nucleoporin, TbNup53b, that has dual localizations within the nucleoplasm and NPC. In addition to association with nucleoporins, TbNup53b interacts with a known trans-splicing component, TSR1, and has a role in controlling expression of surface proteins including the nucleolar periphery-located, procyclin genes. Significantly, while several nucleoporins are implicated in intranuclear transcriptional regulation in metazoa, TbNup53b appears orthologous to components of the yeast/human Nup49/Nup58 complex, for which no transcriptional functions are known. These data suggest that FG-Nups are frequently co-opted to transcriptional functions during evolution and extend the presence of FG-repeat nucleoporin control of gene expression to trypanosomes, suggesting that this is a widespread and ancient eukaryotic feature, as well as underscoring once more flexibility within nucleoporin function. The American Society for Cell Biology 2018-05-01 /pmc/articles/PMC5921576/ /pubmed/29496964 http://dx.doi.org/10.1091/mbc.E17-06-0430 Text en © 2018 Holden et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0/ This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Articles
Holden, Jennifer M.
Koreny, Ludek
Obado, Samson
Ratushny, Alexander V.
Chen, Wei-Ming
Bart, Jean-Mathieu
Navarro, Miguel
Chait, Brian T.
Aitchison, John D.
Rout, Michael P.
Field, Mark C.
Involvement in surface antigen expression by a moonlighting FG-repeat nucleoporin in trypanosomes
title Involvement in surface antigen expression by a moonlighting FG-repeat nucleoporin in trypanosomes
title_full Involvement in surface antigen expression by a moonlighting FG-repeat nucleoporin in trypanosomes
title_fullStr Involvement in surface antigen expression by a moonlighting FG-repeat nucleoporin in trypanosomes
title_full_unstemmed Involvement in surface antigen expression by a moonlighting FG-repeat nucleoporin in trypanosomes
title_short Involvement in surface antigen expression by a moonlighting FG-repeat nucleoporin in trypanosomes
title_sort involvement in surface antigen expression by a moonlighting fg-repeat nucleoporin in trypanosomes
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921576/
https://www.ncbi.nlm.nih.gov/pubmed/29496964
http://dx.doi.org/10.1091/mbc.E17-06-0430
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