Cargando…

Phorbol Ester‐induced G1 Arrest in BALB/MK‐2 Mouse Keratinocytes Is Mediated by δ and η Isoforms of Protein Kinase C

We investigated the possible negative regulation of the cell cycle by protein kinase C (PKC) isoforms in synchronously grown BALB/MK‐2 mouse keratinocytes, in which PKC isoforms were overexpressed by using the adenovirus vector Ax. Cells at the G1/S boundary of the cell cycle were the most sensitive...

Descripción completa

Detalles Bibliográficos
Autores principales: Ishino, Keiko, Ohba, Motoi, Kashiwagi, Mariko, Kawabe, Shoko, Chida, Kazuhiro, Kuroki, Toshio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921714/
https://www.ncbi.nlm.nih.gov/pubmed/9914781
http://dx.doi.org/10.1111/j.1349-7006.1998.tb00507.x
_version_ 1783318070797795328
author Ishino, Keiko
Ohba, Motoi
Kashiwagi, Mariko
Kawabe, Shoko
Chida, Kazuhiro
Kuroki, Toshio
author_facet Ishino, Keiko
Ohba, Motoi
Kashiwagi, Mariko
Kawabe, Shoko
Chida, Kazuhiro
Kuroki, Toshio
author_sort Ishino, Keiko
collection PubMed
description We investigated the possible negative regulation of the cell cycle by protein kinase C (PKC) isoforms in synchronously grown BALB/MK‐2 mouse keratinocytes, in which PKC isoforms were overexpressed by using the adenovirus vector Ax. Cells at the G1/S boundary of the cell cycle were the most sensitive to the inhibitory effect of 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA), a PKC agonist, resulting in G1 arrest. TPA‐induced inhibition of DNA synthesis was augmented by overexpression of the η and δ isoforms, but rescued by the dominant‐negative and antisense η isoforms. In contrast, the α and ζ isoforms showed no effect on DNA synthesis with or without TPA treatment. Immunoblotting indicated cell cycle‐dependent expression of the η isoform, being highest in cells at the G1/S boundary. The present study provides evidence that the η and δ isoforms of PKC are involved in negative regulation of cell cycle at the G1/S boundary in mouse keratinocytes.
format Online
Article
Text
id pubmed-5921714
institution National Center for Biotechnology Information
language English
publishDate 1998
publisher Blackwell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-59217142018-05-11 Phorbol Ester‐induced G1 Arrest in BALB/MK‐2 Mouse Keratinocytes Is Mediated by δ and η Isoforms of Protein Kinase C Ishino, Keiko Ohba, Motoi Kashiwagi, Mariko Kawabe, Shoko Chida, Kazuhiro Kuroki, Toshio Jpn J Cancer Res Article We investigated the possible negative regulation of the cell cycle by protein kinase C (PKC) isoforms in synchronously grown BALB/MK‐2 mouse keratinocytes, in which PKC isoforms were overexpressed by using the adenovirus vector Ax. Cells at the G1/S boundary of the cell cycle were the most sensitive to the inhibitory effect of 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA), a PKC agonist, resulting in G1 arrest. TPA‐induced inhibition of DNA synthesis was augmented by overexpression of the η and δ isoforms, but rescued by the dominant‐negative and antisense η isoforms. In contrast, the α and ζ isoforms showed no effect on DNA synthesis with or without TPA treatment. Immunoblotting indicated cell cycle‐dependent expression of the η isoform, being highest in cells at the G1/S boundary. The present study provides evidence that the η and δ isoforms of PKC are involved in negative regulation of cell cycle at the G1/S boundary in mouse keratinocytes. Blackwell Publishing Ltd 1998-11 /pmc/articles/PMC5921714/ /pubmed/9914781 http://dx.doi.org/10.1111/j.1349-7006.1998.tb00507.x Text en
spellingShingle Article
Ishino, Keiko
Ohba, Motoi
Kashiwagi, Mariko
Kawabe, Shoko
Chida, Kazuhiro
Kuroki, Toshio
Phorbol Ester‐induced G1 Arrest in BALB/MK‐2 Mouse Keratinocytes Is Mediated by δ and η Isoforms of Protein Kinase C
title Phorbol Ester‐induced G1 Arrest in BALB/MK‐2 Mouse Keratinocytes Is Mediated by δ and η Isoforms of Protein Kinase C
title_full Phorbol Ester‐induced G1 Arrest in BALB/MK‐2 Mouse Keratinocytes Is Mediated by δ and η Isoforms of Protein Kinase C
title_fullStr Phorbol Ester‐induced G1 Arrest in BALB/MK‐2 Mouse Keratinocytes Is Mediated by δ and η Isoforms of Protein Kinase C
title_full_unstemmed Phorbol Ester‐induced G1 Arrest in BALB/MK‐2 Mouse Keratinocytes Is Mediated by δ and η Isoforms of Protein Kinase C
title_short Phorbol Ester‐induced G1 Arrest in BALB/MK‐2 Mouse Keratinocytes Is Mediated by δ and η Isoforms of Protein Kinase C
title_sort phorbol ester‐induced g1 arrest in balb/mk‐2 mouse keratinocytes is mediated by δ and η isoforms of protein kinase c
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921714/
https://www.ncbi.nlm.nih.gov/pubmed/9914781
http://dx.doi.org/10.1111/j.1349-7006.1998.tb00507.x
work_keys_str_mv AT ishinokeiko phorbolesterinducedg1arrestinbalbmk2mousekeratinocytesismediatedbydandēisoformsofproteinkinasec
AT ohbamotoi phorbolesterinducedg1arrestinbalbmk2mousekeratinocytesismediatedbydandēisoformsofproteinkinasec
AT kashiwagimariko phorbolesterinducedg1arrestinbalbmk2mousekeratinocytesismediatedbydandēisoformsofproteinkinasec
AT kawabeshoko phorbolesterinducedg1arrestinbalbmk2mousekeratinocytesismediatedbydandēisoformsofproteinkinasec
AT chidakazuhiro phorbolesterinducedg1arrestinbalbmk2mousekeratinocytesismediatedbydandēisoformsofproteinkinasec
AT kurokitoshio phorbolesterinducedg1arrestinbalbmk2mousekeratinocytesismediatedbydandēisoformsofproteinkinasec