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Phorbol Ester‐induced G1 Arrest in BALB/MK‐2 Mouse Keratinocytes Is Mediated by δ and η Isoforms of Protein Kinase C
We investigated the possible negative regulation of the cell cycle by protein kinase C (PKC) isoforms in synchronously grown BALB/MK‐2 mouse keratinocytes, in which PKC isoforms were overexpressed by using the adenovirus vector Ax. Cells at the G1/S boundary of the cell cycle were the most sensitive...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
1998
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921714/ https://www.ncbi.nlm.nih.gov/pubmed/9914781 http://dx.doi.org/10.1111/j.1349-7006.1998.tb00507.x |
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author | Ishino, Keiko Ohba, Motoi Kashiwagi, Mariko Kawabe, Shoko Chida, Kazuhiro Kuroki, Toshio |
author_facet | Ishino, Keiko Ohba, Motoi Kashiwagi, Mariko Kawabe, Shoko Chida, Kazuhiro Kuroki, Toshio |
author_sort | Ishino, Keiko |
collection | PubMed |
description | We investigated the possible negative regulation of the cell cycle by protein kinase C (PKC) isoforms in synchronously grown BALB/MK‐2 mouse keratinocytes, in which PKC isoforms were overexpressed by using the adenovirus vector Ax. Cells at the G1/S boundary of the cell cycle were the most sensitive to the inhibitory effect of 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA), a PKC agonist, resulting in G1 arrest. TPA‐induced inhibition of DNA synthesis was augmented by overexpression of the η and δ isoforms, but rescued by the dominant‐negative and antisense η isoforms. In contrast, the α and ζ isoforms showed no effect on DNA synthesis with or without TPA treatment. Immunoblotting indicated cell cycle‐dependent expression of the η isoform, being highest in cells at the G1/S boundary. The present study provides evidence that the η and δ isoforms of PKC are involved in negative regulation of cell cycle at the G1/S boundary in mouse keratinocytes. |
format | Online Article Text |
id | pubmed-5921714 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1998 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59217142018-05-11 Phorbol Ester‐induced G1 Arrest in BALB/MK‐2 Mouse Keratinocytes Is Mediated by δ and η Isoforms of Protein Kinase C Ishino, Keiko Ohba, Motoi Kashiwagi, Mariko Kawabe, Shoko Chida, Kazuhiro Kuroki, Toshio Jpn J Cancer Res Article We investigated the possible negative regulation of the cell cycle by protein kinase C (PKC) isoforms in synchronously grown BALB/MK‐2 mouse keratinocytes, in which PKC isoforms were overexpressed by using the adenovirus vector Ax. Cells at the G1/S boundary of the cell cycle were the most sensitive to the inhibitory effect of 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA), a PKC agonist, resulting in G1 arrest. TPA‐induced inhibition of DNA synthesis was augmented by overexpression of the η and δ isoforms, but rescued by the dominant‐negative and antisense η isoforms. In contrast, the α and ζ isoforms showed no effect on DNA synthesis with or without TPA treatment. Immunoblotting indicated cell cycle‐dependent expression of the η isoform, being highest in cells at the G1/S boundary. The present study provides evidence that the η and δ isoforms of PKC are involved in negative regulation of cell cycle at the G1/S boundary in mouse keratinocytes. Blackwell Publishing Ltd 1998-11 /pmc/articles/PMC5921714/ /pubmed/9914781 http://dx.doi.org/10.1111/j.1349-7006.1998.tb00507.x Text en |
spellingShingle | Article Ishino, Keiko Ohba, Motoi Kashiwagi, Mariko Kawabe, Shoko Chida, Kazuhiro Kuroki, Toshio Phorbol Ester‐induced G1 Arrest in BALB/MK‐2 Mouse Keratinocytes Is Mediated by δ and η Isoforms of Protein Kinase C |
title | Phorbol Ester‐induced G1 Arrest in BALB/MK‐2 Mouse Keratinocytes Is Mediated by δ and η Isoforms of Protein Kinase C |
title_full | Phorbol Ester‐induced G1 Arrest in BALB/MK‐2 Mouse Keratinocytes Is Mediated by δ and η Isoforms of Protein Kinase C |
title_fullStr | Phorbol Ester‐induced G1 Arrest in BALB/MK‐2 Mouse Keratinocytes Is Mediated by δ and η Isoforms of Protein Kinase C |
title_full_unstemmed | Phorbol Ester‐induced G1 Arrest in BALB/MK‐2 Mouse Keratinocytes Is Mediated by δ and η Isoforms of Protein Kinase C |
title_short | Phorbol Ester‐induced G1 Arrest in BALB/MK‐2 Mouse Keratinocytes Is Mediated by δ and η Isoforms of Protein Kinase C |
title_sort | phorbol ester‐induced g1 arrest in balb/mk‐2 mouse keratinocytes is mediated by δ and η isoforms of protein kinase c |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921714/ https://www.ncbi.nlm.nih.gov/pubmed/9914781 http://dx.doi.org/10.1111/j.1349-7006.1998.tb00507.x |
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