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Mammary Fibroblast‐derived Hepatocyte Growth Factor Stimulates Growth and Morphogenesis of Mouse Mammary Tumor Cells in Primary Culture
We have recently isolated a mammary growth factor from the conditioned medium of mouse mammary stromal fibroblasts and identified it as a mouse homologue of human HGF (hepatocyte growth factor). To elucidate the role of HGF in mouse mammary tumorigenesis, we produced recombinant mouse HGF and examin...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
1998
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921723/ https://www.ncbi.nlm.nih.gov/pubmed/9914782 http://dx.doi.org/10.1111/j.1349-7006.1998.tb00508.x |
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author | Sasaki, Masato Nishio, Masahiro Tsukada, Yasukatsu Enami, Jumpei |
author_facet | Sasaki, Masato Nishio, Masahiro Tsukada, Yasukatsu Enami, Jumpei |
author_sort | Sasaki, Masato |
collection | PubMed |
description | We have recently isolated a mammary growth factor from the conditioned medium of mouse mammary stromal fibroblasts and identified it as a mouse homologue of human HGF (hepatocyte growth factor). To elucidate the role of HGF in mouse mammary tumorigenesis, we produced recombinant mouse HGF and examined its effects on primary cultures of mouse mammary tumor cells in this study. HGF at concentrations above 20 ng/ml maximally stimulated the growth of mammary tumor cells in primary monolayer culture. HGF also stimulated the three‐dimensional growth and branching morphogenesis of mammary tumor cells cultured inside collagen gels. A comparison of the growth‐stimulating activity of HGF with that of EGF (epidermal growth factor) and KGF (keratinocyte growth factor) revealed that HGF is the most potent growth factor among the three. Immunological studies using an antibody against mouse HGF demonstrated that 74% of the growth‐stimulating activity present in the mammary fibroblast‐conditioned medium was abolished by the antibody, indicating that HGF is the major growth factor produced by the fibroblasts. These observations thus suggest a role for HGF as a mammary stromal fibroblast‐derived factor which stimulates growth and morphogenesis of adjacent mammary tumor cells in vivo. |
format | Online Article Text |
id | pubmed-5921723 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1998 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59217232018-05-11 Mammary Fibroblast‐derived Hepatocyte Growth Factor Stimulates Growth and Morphogenesis of Mouse Mammary Tumor Cells in Primary Culture Sasaki, Masato Nishio, Masahiro Tsukada, Yasukatsu Enami, Jumpei Jpn J Cancer Res Article We have recently isolated a mammary growth factor from the conditioned medium of mouse mammary stromal fibroblasts and identified it as a mouse homologue of human HGF (hepatocyte growth factor). To elucidate the role of HGF in mouse mammary tumorigenesis, we produced recombinant mouse HGF and examined its effects on primary cultures of mouse mammary tumor cells in this study. HGF at concentrations above 20 ng/ml maximally stimulated the growth of mammary tumor cells in primary monolayer culture. HGF also stimulated the three‐dimensional growth and branching morphogenesis of mammary tumor cells cultured inside collagen gels. A comparison of the growth‐stimulating activity of HGF with that of EGF (epidermal growth factor) and KGF (keratinocyte growth factor) revealed that HGF is the most potent growth factor among the three. Immunological studies using an antibody against mouse HGF demonstrated that 74% of the growth‐stimulating activity present in the mammary fibroblast‐conditioned medium was abolished by the antibody, indicating that HGF is the major growth factor produced by the fibroblasts. These observations thus suggest a role for HGF as a mammary stromal fibroblast‐derived factor which stimulates growth and morphogenesis of adjacent mammary tumor cells in vivo. Blackwell Publishing Ltd 1998-11 /pmc/articles/PMC5921723/ /pubmed/9914782 http://dx.doi.org/10.1111/j.1349-7006.1998.tb00508.x Text en |
spellingShingle | Article Sasaki, Masato Nishio, Masahiro Tsukada, Yasukatsu Enami, Jumpei Mammary Fibroblast‐derived Hepatocyte Growth Factor Stimulates Growth and Morphogenesis of Mouse Mammary Tumor Cells in Primary Culture |
title | Mammary Fibroblast‐derived Hepatocyte Growth Factor Stimulates Growth and Morphogenesis of Mouse Mammary Tumor Cells in Primary Culture |
title_full | Mammary Fibroblast‐derived Hepatocyte Growth Factor Stimulates Growth and Morphogenesis of Mouse Mammary Tumor Cells in Primary Culture |
title_fullStr | Mammary Fibroblast‐derived Hepatocyte Growth Factor Stimulates Growth and Morphogenesis of Mouse Mammary Tumor Cells in Primary Culture |
title_full_unstemmed | Mammary Fibroblast‐derived Hepatocyte Growth Factor Stimulates Growth and Morphogenesis of Mouse Mammary Tumor Cells in Primary Culture |
title_short | Mammary Fibroblast‐derived Hepatocyte Growth Factor Stimulates Growth and Morphogenesis of Mouse Mammary Tumor Cells in Primary Culture |
title_sort | mammary fibroblast‐derived hepatocyte growth factor stimulates growth and morphogenesis of mouse mammary tumor cells in primary culture |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921723/ https://www.ncbi.nlm.nih.gov/pubmed/9914782 http://dx.doi.org/10.1111/j.1349-7006.1998.tb00508.x |
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