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Cyclin D1 Overexpression Detected by a Simple Competitive Reverse Transcription‐polymerase Chain Reaction Assay for Lymphoid Malignancies

t(11;14)(q13;q32) observed in B‐cell malignancies is associated with cyclin D1 (bcl‐1, PRAD1, CCND1) overexpression. We devised a simple competitive reverse transcription‐polymerase chain reaction (RT‐PCR) assay for rapid detection of cyclin D1 overexpression. Sharing a single upstream primer derive...

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Autores principales: Taniguchi, Toshiyasu, Fujita, Akira, Takahashi, Shunji, Uchimaru, Kaoru, Yoshikawa, Miwa, Asano, Shigetaka, Fujita, Toshiro, Motokura, Toru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921770/
https://www.ncbi.nlm.nih.gov/pubmed/9548443
http://dx.doi.org/10.1111/j.1349-7006.1998.tb00544.x
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author Taniguchi, Toshiyasu
Fujita, Akira
Takahashi, Shunji
Uchimaru, Kaoru
Yoshikawa, Miwa
Asano, Shigetaka
Fujita, Toshiro
Motokura, Toru
author_facet Taniguchi, Toshiyasu
Fujita, Akira
Takahashi, Shunji
Uchimaru, Kaoru
Yoshikawa, Miwa
Asano, Shigetaka
Fujita, Toshiro
Motokura, Toru
author_sort Taniguchi, Toshiyasu
collection PubMed
description t(11;14)(q13;q32) observed in B‐cell malignancies is associated with cyclin D1 (bcl‐1, PRAD1, CCND1) overexpression. We devised a simple competitive reverse transcription‐polymerase chain reaction (RT‐PCR) assay for rapid detection of cyclin D1 overexpression. Sharing a single upstream primer derived from a homologous sequence in cyclins D1, D2 and D3, each PCR product serves as a competitor and cyclin D1 overexpression is determined by comparing the intensities of the three amplified products. We analyzed cyclin D1 in clinical specimens from 104 patients with lymphoid malignancies. Cyclin D1 overexpression was evident in 13 of 104 (7/72 non‐Hodgkin's lymphomas, 0/6 adult T‐cell lymphoma/leukemias, 0/4 Hodgkin's diseases, 0/11 acute lymphoblastic leukemias, 3/4 multiple myelomas, 1/2 Waldenströmas, macroglobulinemias, 1/2 prolymphocytic leukemias and 1/3 chronic lymphocytic leukemias). Among 72 patients for whom cytogenetic studies had been done, all 7 patients with t(11;14) were positive. The relative expression levels of D‐type cyclins altered dramatically in the presence of t(11;14). Thus, this RT‐PCR assay can identify tumors with cyclin D1 overexpression. Cyclin D1 overexpression was frequent in extranodal specimens (11 out of 32 vs. 2 of 72 lymph nodes) and was restricted to specific types of lymphoid malignancies, as observed using other methods. This reliable assay should be suitable to provide clinical guidance for the diagnosis and management of lymphoid malignancies, especially in the case of extranodal involvement.
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spelling pubmed-59217702018-05-11 Cyclin D1 Overexpression Detected by a Simple Competitive Reverse Transcription‐polymerase Chain Reaction Assay for Lymphoid Malignancies Taniguchi, Toshiyasu Fujita, Akira Takahashi, Shunji Uchimaru, Kaoru Yoshikawa, Miwa Asano, Shigetaka Fujita, Toshiro Motokura, Toru Jpn J Cancer Res Article t(11;14)(q13;q32) observed in B‐cell malignancies is associated with cyclin D1 (bcl‐1, PRAD1, CCND1) overexpression. We devised a simple competitive reverse transcription‐polymerase chain reaction (RT‐PCR) assay for rapid detection of cyclin D1 overexpression. Sharing a single upstream primer derived from a homologous sequence in cyclins D1, D2 and D3, each PCR product serves as a competitor and cyclin D1 overexpression is determined by comparing the intensities of the three amplified products. We analyzed cyclin D1 in clinical specimens from 104 patients with lymphoid malignancies. Cyclin D1 overexpression was evident in 13 of 104 (7/72 non‐Hodgkin's lymphomas, 0/6 adult T‐cell lymphoma/leukemias, 0/4 Hodgkin's diseases, 0/11 acute lymphoblastic leukemias, 3/4 multiple myelomas, 1/2 Waldenströmas, macroglobulinemias, 1/2 prolymphocytic leukemias and 1/3 chronic lymphocytic leukemias). Among 72 patients for whom cytogenetic studies had been done, all 7 patients with t(11;14) were positive. The relative expression levels of D‐type cyclins altered dramatically in the presence of t(11;14). Thus, this RT‐PCR assay can identify tumors with cyclin D1 overexpression. Cyclin D1 overexpression was frequent in extranodal specimens (11 out of 32 vs. 2 of 72 lymph nodes) and was restricted to specific types of lymphoid malignancies, as observed using other methods. This reliable assay should be suitable to provide clinical guidance for the diagnosis and management of lymphoid malignancies, especially in the case of extranodal involvement. Blackwell Publishing Ltd 1998-02 /pmc/articles/PMC5921770/ /pubmed/9548443 http://dx.doi.org/10.1111/j.1349-7006.1998.tb00544.x Text en
spellingShingle Article
Taniguchi, Toshiyasu
Fujita, Akira
Takahashi, Shunji
Uchimaru, Kaoru
Yoshikawa, Miwa
Asano, Shigetaka
Fujita, Toshiro
Motokura, Toru
Cyclin D1 Overexpression Detected by a Simple Competitive Reverse Transcription‐polymerase Chain Reaction Assay for Lymphoid Malignancies
title Cyclin D1 Overexpression Detected by a Simple Competitive Reverse Transcription‐polymerase Chain Reaction Assay for Lymphoid Malignancies
title_full Cyclin D1 Overexpression Detected by a Simple Competitive Reverse Transcription‐polymerase Chain Reaction Assay for Lymphoid Malignancies
title_fullStr Cyclin D1 Overexpression Detected by a Simple Competitive Reverse Transcription‐polymerase Chain Reaction Assay for Lymphoid Malignancies
title_full_unstemmed Cyclin D1 Overexpression Detected by a Simple Competitive Reverse Transcription‐polymerase Chain Reaction Assay for Lymphoid Malignancies
title_short Cyclin D1 Overexpression Detected by a Simple Competitive Reverse Transcription‐polymerase Chain Reaction Assay for Lymphoid Malignancies
title_sort cyclin d1 overexpression detected by a simple competitive reverse transcription‐polymerase chain reaction assay for lymphoid malignancies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921770/
https://www.ncbi.nlm.nih.gov/pubmed/9548443
http://dx.doi.org/10.1111/j.1349-7006.1998.tb00544.x
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