Establishment of Epstein‐Barr Virus‐positive Human Gastric Epithelial Cell Lines

We have established two Epstein‐Barr virus (EBV)‐positive cell lines, GT38 and GT39, derived from human gastric tissues of two patients bearing gastric carcinoma. Both cell lines were positive for cytokeratin, an epithelial marker, but not for lymphocyte‐related markers. Unlike GT39 cell line, GT38...

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Detalles Bibliográficos
Autores principales: Tajima, Masako, Komuro, Masato, Okinaga, Kota
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1998
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921807/
https://www.ncbi.nlm.nih.gov/pubmed/9600119
http://dx.doi.org/10.1111/j.1349-7006.1998.tb00557.x
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author Tajima, Masako
Komuro, Masato
Okinaga, Kota
author_facet Tajima, Masako
Komuro, Masato
Okinaga, Kota
author_sort Tajima, Masako
collection PubMed
description We have established two Epstein‐Barr virus (EBV)‐positive cell lines, GT38 and GT39, derived from human gastric tissues of two patients bearing gastric carcinoma. Both cell lines were positive for cytokeratin, an epithelial marker, but not for lymphocyte‐related markers. Unlike GT39 cell line, GT38 cells lacked the property of contact inhibition. EBV genome was detected in both cell lines. The cell lines were positive for latent membrane protein 1, and EBV‐determined nuclear antigen 1 (EBNA1). EBNA2 was also detected in GT38. These cell lines should be useful for studying the interaction of EBV with gastric epithelial cells and its role in gastric carcinogenesis.
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spelling pubmed-59218072018-05-11 Establishment of Epstein‐Barr Virus‐positive Human Gastric Epithelial Cell Lines Tajima, Masako Komuro, Masato Okinaga, Kota Jpn J Cancer Res Article We have established two Epstein‐Barr virus (EBV)‐positive cell lines, GT38 and GT39, derived from human gastric tissues of two patients bearing gastric carcinoma. Both cell lines were positive for cytokeratin, an epithelial marker, but not for lymphocyte‐related markers. Unlike GT39 cell line, GT38 cells lacked the property of contact inhibition. EBV genome was detected in both cell lines. The cell lines were positive for latent membrane protein 1, and EBV‐determined nuclear antigen 1 (EBNA1). EBNA2 was also detected in GT38. These cell lines should be useful for studying the interaction of EBV with gastric epithelial cells and its role in gastric carcinogenesis. Blackwell Publishing Ltd 1998-03 /pmc/articles/PMC5921807/ /pubmed/9600119 http://dx.doi.org/10.1111/j.1349-7006.1998.tb00557.x Text en
spellingShingle Article
Tajima, Masako
Komuro, Masato
Okinaga, Kota
Establishment of Epstein‐Barr Virus‐positive Human Gastric Epithelial Cell Lines
title Establishment of Epstein‐Barr Virus‐positive Human Gastric Epithelial Cell Lines
title_full Establishment of Epstein‐Barr Virus‐positive Human Gastric Epithelial Cell Lines
title_fullStr Establishment of Epstein‐Barr Virus‐positive Human Gastric Epithelial Cell Lines
title_full_unstemmed Establishment of Epstein‐Barr Virus‐positive Human Gastric Epithelial Cell Lines
title_short Establishment of Epstein‐Barr Virus‐positive Human Gastric Epithelial Cell Lines
title_sort establishment of epstein‐barr virus‐positive human gastric epithelial cell lines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921807/
https://www.ncbi.nlm.nih.gov/pubmed/9600119
http://dx.doi.org/10.1111/j.1349-7006.1998.tb00557.x
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