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Expression and Localization of Ornithine Decarboxylase in Reversible Papillomatosis Induced by Uracil in Rat Bladder

Direct mechanical irritation by uracil calculi formed following feeding of 3% uracil in the diet to male rats produces severe papillary hyperplasia (papillomatosis, which is reversible) of bladder epithelium. To evaluate the mechanism of the appearance of uracil‐induced papillomatosis, we examined t...

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Autores principales: Takada, Nobuyasu, Yano, Yoshihisa, Otori, Kazuhiko, Otani, Shuzo, Nomura, Shintaro, Kitamura, Yukihiko, Fukushima, Shoji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921823/
https://www.ncbi.nlm.nih.gov/pubmed/9617342
http://dx.doi.org/10.1111/j.1349-7006.1998.tb00574.x
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author Takada, Nobuyasu
Yano, Yoshihisa
Otori, Kazuhiko
Otani, Shuzo
Nomura, Shintaro
Kitamura, Yukihiko
Fukushima, Shoji
author_facet Takada, Nobuyasu
Yano, Yoshihisa
Otori, Kazuhiko
Otani, Shuzo
Nomura, Shintaro
Kitamura, Yukihiko
Fukushima, Shoji
author_sort Takada, Nobuyasu
collection PubMed
description Direct mechanical irritation by uracil calculi formed following feeding of 3% uracil in the diet to male rats produces severe papillary hyperplasia (papillomatosis, which is reversible) of bladder epithelium. To evaluate the mechanism of the appearance of uracil‐induced papillomatosis, we examined the changes of the enzyme activity and the localization of ornithine decarboxylase (ODC), as well as polyamine biosynthesis, and epithelial proliferation, that accompany the sequential bladder epithelial changes following administration and withdrawal of uracil. Moreover, expression of ODC mRNA was investigated using northern blotting and localization of ODC mRNA was demonstrated using in situ hybridization. ODC activity during uracil administration was maintained at a high level compared to that in normal epithelium, but sharply decreased after cessation of uracil treatment. The accumulation of ODC protein was observed in the proliferating bladder epithelium by immunohistochemical examination and western blotting analysis, and even after cessation of treatment, the protein binding to anti‐ODC antibody remained mildly elevated. Sequential changes of proliferating cell nuclear antigen (PCNA)‐positive cells in the epithelium during the development and disappearance of papillomatosis correlated with ODC activity. ODC mRNA was expressed strongly in the proliferating epithelium in rats treated with uracil and weakly in normal epithelium, in accordance with the location of ODC protein. Consequently, our data demonstrate that cell proliferation in the development of papillomatosis is closely associated with polyamine metabolism, and moreover suggest that ODC activity is up‐regulated at a post‐translational step.
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spelling pubmed-59218232018-05-11 Expression and Localization of Ornithine Decarboxylase in Reversible Papillomatosis Induced by Uracil in Rat Bladder Takada, Nobuyasu Yano, Yoshihisa Otori, Kazuhiko Otani, Shuzo Nomura, Shintaro Kitamura, Yukihiko Fukushima, Shoji Jpn J Cancer Res Article Direct mechanical irritation by uracil calculi formed following feeding of 3% uracil in the diet to male rats produces severe papillary hyperplasia (papillomatosis, which is reversible) of bladder epithelium. To evaluate the mechanism of the appearance of uracil‐induced papillomatosis, we examined the changes of the enzyme activity and the localization of ornithine decarboxylase (ODC), as well as polyamine biosynthesis, and epithelial proliferation, that accompany the sequential bladder epithelial changes following administration and withdrawal of uracil. Moreover, expression of ODC mRNA was investigated using northern blotting and localization of ODC mRNA was demonstrated using in situ hybridization. ODC activity during uracil administration was maintained at a high level compared to that in normal epithelium, but sharply decreased after cessation of uracil treatment. The accumulation of ODC protein was observed in the proliferating bladder epithelium by immunohistochemical examination and western blotting analysis, and even after cessation of treatment, the protein binding to anti‐ODC antibody remained mildly elevated. Sequential changes of proliferating cell nuclear antigen (PCNA)‐positive cells in the epithelium during the development and disappearance of papillomatosis correlated with ODC activity. ODC mRNA was expressed strongly in the proliferating epithelium in rats treated with uracil and weakly in normal epithelium, in accordance with the location of ODC protein. Consequently, our data demonstrate that cell proliferation in the development of papillomatosis is closely associated with polyamine metabolism, and moreover suggest that ODC activity is up‐regulated at a post‐translational step. Blackwell Publishing Ltd 1998-04 /pmc/articles/PMC5921823/ /pubmed/9617342 http://dx.doi.org/10.1111/j.1349-7006.1998.tb00574.x Text en
spellingShingle Article
Takada, Nobuyasu
Yano, Yoshihisa
Otori, Kazuhiko
Otani, Shuzo
Nomura, Shintaro
Kitamura, Yukihiko
Fukushima, Shoji
Expression and Localization of Ornithine Decarboxylase in Reversible Papillomatosis Induced by Uracil in Rat Bladder
title Expression and Localization of Ornithine Decarboxylase in Reversible Papillomatosis Induced by Uracil in Rat Bladder
title_full Expression and Localization of Ornithine Decarboxylase in Reversible Papillomatosis Induced by Uracil in Rat Bladder
title_fullStr Expression and Localization of Ornithine Decarboxylase in Reversible Papillomatosis Induced by Uracil in Rat Bladder
title_full_unstemmed Expression and Localization of Ornithine Decarboxylase in Reversible Papillomatosis Induced by Uracil in Rat Bladder
title_short Expression and Localization of Ornithine Decarboxylase in Reversible Papillomatosis Induced by Uracil in Rat Bladder
title_sort expression and localization of ornithine decarboxylase in reversible papillomatosis induced by uracil in rat bladder
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921823/
https://www.ncbi.nlm.nih.gov/pubmed/9617342
http://dx.doi.org/10.1111/j.1349-7006.1998.tb00574.x
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