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Impact of pigment epithelium-derived factor on colorectal cancer in vitro and in vivo
Pigment epithelial derived factor (PEDF) is a secreted glycoprotein that is a non-inhibitory member of the serine protease inhibitor (serpin) family. PEDF exhibits multiple biological properties including neuroprotective, anti-angiogenic, and immune-modulating. Interestingly, PEDF exerts the inhibit...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5922387/ https://www.ncbi.nlm.nih.gov/pubmed/29721193 http://dx.doi.org/10.18632/oncotarget.24953 |
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author | Harries, Rhiannon L. Owen, Sioned Ruge, Fiona Morgan, Meleri Li, Jun Zhang, Zhangtao Harding, Keith G. Torkington, Jared Jiang, Wen G. Cai, Jun |
author_facet | Harries, Rhiannon L. Owen, Sioned Ruge, Fiona Morgan, Meleri Li, Jun Zhang, Zhangtao Harding, Keith G. Torkington, Jared Jiang, Wen G. Cai, Jun |
author_sort | Harries, Rhiannon L. |
collection | PubMed |
description | Pigment epithelial derived factor (PEDF) is a secreted glycoprotein that is a non-inhibitory member of the serine protease inhibitor (serpin) family. PEDF exhibits multiple biological properties including neuroprotective, anti-angiogenic, and immune-modulating. Interestingly, PEDF exerts the inhibitory effects in cancers derived from certain tissues, including prostatic, ovarian, and pancreatic carcinomas. The current study aimed to elucidate its role in colorectal cancer development. PEDF expression in human colorectal cancer tissue was assessed using quantitative polymerase chain reaction (qPCR) and immunohistochemical staining (IHC). The effect of treatment with recombinant PEDF on cellular function was examined using in vitro functional assays. PEDF expression was downregulated in colorectal cancer cell tissue. Treatment with recombinant PEDF resulted in significant decreases in the rate of colorectal cancer cell migration and invasion and an increase in cellular adhesion in colorectal cancer cell lines examined. These results indicate that upregulation of PEDF expression may serve as a new strategy for further investigation of therapeutic relevance to the prevention of the metastatic spread of colorectal cancer. |
format | Online Article Text |
id | pubmed-5922387 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-59223872018-05-02 Impact of pigment epithelium-derived factor on colorectal cancer in vitro and in vivo Harries, Rhiannon L. Owen, Sioned Ruge, Fiona Morgan, Meleri Li, Jun Zhang, Zhangtao Harding, Keith G. Torkington, Jared Jiang, Wen G. Cai, Jun Oncotarget Research Paper Pigment epithelial derived factor (PEDF) is a secreted glycoprotein that is a non-inhibitory member of the serine protease inhibitor (serpin) family. PEDF exhibits multiple biological properties including neuroprotective, anti-angiogenic, and immune-modulating. Interestingly, PEDF exerts the inhibitory effects in cancers derived from certain tissues, including prostatic, ovarian, and pancreatic carcinomas. The current study aimed to elucidate its role in colorectal cancer development. PEDF expression in human colorectal cancer tissue was assessed using quantitative polymerase chain reaction (qPCR) and immunohistochemical staining (IHC). The effect of treatment with recombinant PEDF on cellular function was examined using in vitro functional assays. PEDF expression was downregulated in colorectal cancer cell tissue. Treatment with recombinant PEDF resulted in significant decreases in the rate of colorectal cancer cell migration and invasion and an increase in cellular adhesion in colorectal cancer cell lines examined. These results indicate that upregulation of PEDF expression may serve as a new strategy for further investigation of therapeutic relevance to the prevention of the metastatic spread of colorectal cancer. Impact Journals LLC 2018-04-10 /pmc/articles/PMC5922387/ /pubmed/29721193 http://dx.doi.org/10.18632/oncotarget.24953 Text en Copyright: © 2018 Harries et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Harries, Rhiannon L. Owen, Sioned Ruge, Fiona Morgan, Meleri Li, Jun Zhang, Zhangtao Harding, Keith G. Torkington, Jared Jiang, Wen G. Cai, Jun Impact of pigment epithelium-derived factor on colorectal cancer in vitro and in vivo |
title | Impact of pigment epithelium-derived factor on colorectal cancer in vitro and in vivo |
title_full | Impact of pigment epithelium-derived factor on colorectal cancer in vitro and in vivo |
title_fullStr | Impact of pigment epithelium-derived factor on colorectal cancer in vitro and in vivo |
title_full_unstemmed | Impact of pigment epithelium-derived factor on colorectal cancer in vitro and in vivo |
title_short | Impact of pigment epithelium-derived factor on colorectal cancer in vitro and in vivo |
title_sort | impact of pigment epithelium-derived factor on colorectal cancer in vitro and in vivo |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5922387/ https://www.ncbi.nlm.nih.gov/pubmed/29721193 http://dx.doi.org/10.18632/oncotarget.24953 |
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