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IMPACT OF TGF-β FAMILY-RELATED GROWTH FACTORS ON CHONDROGENIC DIFFERENTIATION OF ADIPOSE-DERIVED STEM CELLS ISOLATED FROM LIPOASPIRATES AND INFRAPATELLAR FAT PADS OF OSTEOARTHRITIC PATIENTS

The success of cell-based approaches for the treatment of cartilage defects requires an optimal autologous cell source with chondrogenic differentiation ability that maintains its differentiated properties and stability following implantation. The objective of this study was to compare the chondroge...

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Autores principales: López-Ruiz, E., Jiménez, G., Kwiatkowski, W., Montañez, E., Arrebola, F., Carrillo, E., Choe, S., Marchal, J.A., Perán, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5922762/
https://www.ncbi.nlm.nih.gov/pubmed/29652075
http://dx.doi.org/10.22203/eCM.v035a15
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author López-Ruiz, E.
Jiménez, G.
Kwiatkowski, W.
Montañez, E.
Arrebola, F.
Carrillo, E.
Choe, S.
Marchal, J.A.
Perán, M.
author_facet López-Ruiz, E.
Jiménez, G.
Kwiatkowski, W.
Montañez, E.
Arrebola, F.
Carrillo, E.
Choe, S.
Marchal, J.A.
Perán, M.
author_sort López-Ruiz, E.
collection PubMed
description The success of cell-based approaches for the treatment of cartilage defects requires an optimal autologous cell source with chondrogenic differentiation ability that maintains its differentiated properties and stability following implantation. The objective of this study was to compare the chondrogenic capacity of mesenchymal stem cells (MSCs) isolated from lipoaspirates (ASCs) and infrapatellar fat pads (IFPSCs) of osteoarthritic patients and treated with transforming growth factor (TGF)-β family-related growth factors. Cells were cultured for 6 weeks in a 3D pellet culture system with the chimeric activin A/bone morphogenic protein (BMP)-2 ligand (AB235), the chimeric nodal/BMP-2 ligand (NB260) or BMP-2. To investigate the stability of the new cartilage, ASCs-treated pellets were transplanted subcutaneously into severe combined immunodeficiency (SCID) mice. Histological and immunohistochemical assessment confirmed that the growth factors induced cartilage differentiation in both isolated cell types. However, reverse transcription-quantitative PCR results showed that ASCs presented a higher chondrogenic potential than IFPSCs. In vivo results revealed that AB235-treated ASCs pellets were larger in size and could form stable cartilage-like tissue as compared to NB260-treated pellets, while BMP-2-treated pellets underwent calcification. The chondrogenic induction of ASCs by AB235 treatment was mediated by SMAD2/3 activation, as proved by immunofluorescence analysis. The results of this study indicated that the combination of ASCs and AB235 might lead to a cell-based cartilage regeneration treatment.
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spelling pubmed-59227622018-07-13 IMPACT OF TGF-β FAMILY-RELATED GROWTH FACTORS ON CHONDROGENIC DIFFERENTIATION OF ADIPOSE-DERIVED STEM CELLS ISOLATED FROM LIPOASPIRATES AND INFRAPATELLAR FAT PADS OF OSTEOARTHRITIC PATIENTS López-Ruiz, E. Jiménez, G. Kwiatkowski, W. Montañez, E. Arrebola, F. Carrillo, E. Choe, S. Marchal, J.A. Perán, M. Eur Cell Mater Article The success of cell-based approaches for the treatment of cartilage defects requires an optimal autologous cell source with chondrogenic differentiation ability that maintains its differentiated properties and stability following implantation. The objective of this study was to compare the chondrogenic capacity of mesenchymal stem cells (MSCs) isolated from lipoaspirates (ASCs) and infrapatellar fat pads (IFPSCs) of osteoarthritic patients and treated with transforming growth factor (TGF)-β family-related growth factors. Cells were cultured for 6 weeks in a 3D pellet culture system with the chimeric activin A/bone morphogenic protein (BMP)-2 ligand (AB235), the chimeric nodal/BMP-2 ligand (NB260) or BMP-2. To investigate the stability of the new cartilage, ASCs-treated pellets were transplanted subcutaneously into severe combined immunodeficiency (SCID) mice. Histological and immunohistochemical assessment confirmed that the growth factors induced cartilage differentiation in both isolated cell types. However, reverse transcription-quantitative PCR results showed that ASCs presented a higher chondrogenic potential than IFPSCs. In vivo results revealed that AB235-treated ASCs pellets were larger in size and could form stable cartilage-like tissue as compared to NB260-treated pellets, while BMP-2-treated pellets underwent calcification. The chondrogenic induction of ASCs by AB235 treatment was mediated by SMAD2/3 activation, as proved by immunofluorescence analysis. The results of this study indicated that the combination of ASCs and AB235 might lead to a cell-based cartilage regeneration treatment. 2018-04-13 /pmc/articles/PMC5922762/ /pubmed/29652075 http://dx.doi.org/10.22203/eCM.v035a15 Text en This article is distributed in accordance with Creative Commons Attribution Licence (http://creativecommons.org/licenses/by-sa/4.0/).
spellingShingle Article
López-Ruiz, E.
Jiménez, G.
Kwiatkowski, W.
Montañez, E.
Arrebola, F.
Carrillo, E.
Choe, S.
Marchal, J.A.
Perán, M.
IMPACT OF TGF-β FAMILY-RELATED GROWTH FACTORS ON CHONDROGENIC DIFFERENTIATION OF ADIPOSE-DERIVED STEM CELLS ISOLATED FROM LIPOASPIRATES AND INFRAPATELLAR FAT PADS OF OSTEOARTHRITIC PATIENTS
title IMPACT OF TGF-β FAMILY-RELATED GROWTH FACTORS ON CHONDROGENIC DIFFERENTIATION OF ADIPOSE-DERIVED STEM CELLS ISOLATED FROM LIPOASPIRATES AND INFRAPATELLAR FAT PADS OF OSTEOARTHRITIC PATIENTS
title_full IMPACT OF TGF-β FAMILY-RELATED GROWTH FACTORS ON CHONDROGENIC DIFFERENTIATION OF ADIPOSE-DERIVED STEM CELLS ISOLATED FROM LIPOASPIRATES AND INFRAPATELLAR FAT PADS OF OSTEOARTHRITIC PATIENTS
title_fullStr IMPACT OF TGF-β FAMILY-RELATED GROWTH FACTORS ON CHONDROGENIC DIFFERENTIATION OF ADIPOSE-DERIVED STEM CELLS ISOLATED FROM LIPOASPIRATES AND INFRAPATELLAR FAT PADS OF OSTEOARTHRITIC PATIENTS
title_full_unstemmed IMPACT OF TGF-β FAMILY-RELATED GROWTH FACTORS ON CHONDROGENIC DIFFERENTIATION OF ADIPOSE-DERIVED STEM CELLS ISOLATED FROM LIPOASPIRATES AND INFRAPATELLAR FAT PADS OF OSTEOARTHRITIC PATIENTS
title_short IMPACT OF TGF-β FAMILY-RELATED GROWTH FACTORS ON CHONDROGENIC DIFFERENTIATION OF ADIPOSE-DERIVED STEM CELLS ISOLATED FROM LIPOASPIRATES AND INFRAPATELLAR FAT PADS OF OSTEOARTHRITIC PATIENTS
title_sort impact of tgf-β family-related growth factors on chondrogenic differentiation of adipose-derived stem cells isolated from lipoaspirates and infrapatellar fat pads of osteoarthritic patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5922762/
https://www.ncbi.nlm.nih.gov/pubmed/29652075
http://dx.doi.org/10.22203/eCM.v035a15
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