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Bacteria as living patchy colloids: Phenotypic heterogeneity in surface adhesion

Understanding and controlling the surface adhesion of pathogenic bacteria is of urgent biomedical importance. However, many aspects of this process remain unclear (for example, microscopic details of the initial adhesion and possible variations between individual cells). Using a new high-throughput...

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Autores principales: Vissers, Teun, Brown, Aidan T., Koumakis, Nick, Dawson, Angela, Hermes, Michiel, Schwarz-Linek, Jana, Schofield, Andrew B., French, Joseph M., Koutsos, Vasileios, Arlt, Jochen, Martinez, Vincent A., Poon, Wilson C. K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5922800/
https://www.ncbi.nlm.nih.gov/pubmed/29719861
http://dx.doi.org/10.1126/sciadv.aao1170
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author Vissers, Teun
Brown, Aidan T.
Koumakis, Nick
Dawson, Angela
Hermes, Michiel
Schwarz-Linek, Jana
Schofield, Andrew B.
French, Joseph M.
Koutsos, Vasileios
Arlt, Jochen
Martinez, Vincent A.
Poon, Wilson C. K.
author_facet Vissers, Teun
Brown, Aidan T.
Koumakis, Nick
Dawson, Angela
Hermes, Michiel
Schwarz-Linek, Jana
Schofield, Andrew B.
French, Joseph M.
Koutsos, Vasileios
Arlt, Jochen
Martinez, Vincent A.
Poon, Wilson C. K.
author_sort Vissers, Teun
collection PubMed
description Understanding and controlling the surface adhesion of pathogenic bacteria is of urgent biomedical importance. However, many aspects of this process remain unclear (for example, microscopic details of the initial adhesion and possible variations between individual cells). Using a new high-throughput method, we identify and follow many single cells within a clonal population of Escherichia coli near a glass surface. We find strong phenotypic heterogeneities: A fraction of the cells remain in the free (planktonic) state, whereas others adhere with an adhesion strength that itself exhibits phenotypic heterogeneity. We explain our observations using a patchy colloid model; cells bind with localized, adhesive patches, and the strength of adhesion is determined by the number of patches: Nonadherers have no patches, weak adherers bind with a single patch only, and strong adherers bind via a single or multiple patches. We discuss possible implications of our results for controlling bacterial adhesion in biomedical and other applications.
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spelling pubmed-59228002018-05-01 Bacteria as living patchy colloids: Phenotypic heterogeneity in surface adhesion Vissers, Teun Brown, Aidan T. Koumakis, Nick Dawson, Angela Hermes, Michiel Schwarz-Linek, Jana Schofield, Andrew B. French, Joseph M. Koutsos, Vasileios Arlt, Jochen Martinez, Vincent A. Poon, Wilson C. K. Sci Adv Research Articles Understanding and controlling the surface adhesion of pathogenic bacteria is of urgent biomedical importance. However, many aspects of this process remain unclear (for example, microscopic details of the initial adhesion and possible variations between individual cells). Using a new high-throughput method, we identify and follow many single cells within a clonal population of Escherichia coli near a glass surface. We find strong phenotypic heterogeneities: A fraction of the cells remain in the free (planktonic) state, whereas others adhere with an adhesion strength that itself exhibits phenotypic heterogeneity. We explain our observations using a patchy colloid model; cells bind with localized, adhesive patches, and the strength of adhesion is determined by the number of patches: Nonadherers have no patches, weak adherers bind with a single patch only, and strong adherers bind via a single or multiple patches. We discuss possible implications of our results for controlling bacterial adhesion in biomedical and other applications. American Association for the Advancement of Science 2018-04-27 /pmc/articles/PMC5922800/ /pubmed/29719861 http://dx.doi.org/10.1126/sciadv.aao1170 Text en Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (http://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Research Articles
Vissers, Teun
Brown, Aidan T.
Koumakis, Nick
Dawson, Angela
Hermes, Michiel
Schwarz-Linek, Jana
Schofield, Andrew B.
French, Joseph M.
Koutsos, Vasileios
Arlt, Jochen
Martinez, Vincent A.
Poon, Wilson C. K.
Bacteria as living patchy colloids: Phenotypic heterogeneity in surface adhesion
title Bacteria as living patchy colloids: Phenotypic heterogeneity in surface adhesion
title_full Bacteria as living patchy colloids: Phenotypic heterogeneity in surface adhesion
title_fullStr Bacteria as living patchy colloids: Phenotypic heterogeneity in surface adhesion
title_full_unstemmed Bacteria as living patchy colloids: Phenotypic heterogeneity in surface adhesion
title_short Bacteria as living patchy colloids: Phenotypic heterogeneity in surface adhesion
title_sort bacteria as living patchy colloids: phenotypic heterogeneity in surface adhesion
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5922800/
https://www.ncbi.nlm.nih.gov/pubmed/29719861
http://dx.doi.org/10.1126/sciadv.aao1170
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