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A robust and tunable mitotic oscillator in artificial cells

Single-cell analysis is pivotal to deciphering complex phenomena like heterogeneity, bistability, and asynchronous oscillations, where a population ensemble cannot represent individual behaviors. Bulk cell-free systems, despite having unique advantages of manipulation and characterization of biochem...

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Autores principales: Guan, Ye, Li, Zhengda, Wang, Shiyuan, Barnes, Patrick M, Liu, Xuwen, Xu, Haotian, Jin, Minjun, Liu, Allen P, Yang, Qiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5922972/
https://www.ncbi.nlm.nih.gov/pubmed/29620527
http://dx.doi.org/10.7554/eLife.33549
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author Guan, Ye
Li, Zhengda
Wang, Shiyuan
Barnes, Patrick M
Liu, Xuwen
Xu, Haotian
Jin, Minjun
Liu, Allen P
Yang, Qiong
author_facet Guan, Ye
Li, Zhengda
Wang, Shiyuan
Barnes, Patrick M
Liu, Xuwen
Xu, Haotian
Jin, Minjun
Liu, Allen P
Yang, Qiong
author_sort Guan, Ye
collection PubMed
description Single-cell analysis is pivotal to deciphering complex phenomena like heterogeneity, bistability, and asynchronous oscillations, where a population ensemble cannot represent individual behaviors. Bulk cell-free systems, despite having unique advantages of manipulation and characterization of biochemical networks, lack the essential single-cell information to understand a class of out-of-steady-state dynamics including cell cycles. Here, by encapsulating Xenopus egg extracts in water-in-oil microemulsions, we developed artificial cells that are adjustable in sizes and periods, sustain mitotic oscillations for over 30 cycles, and function in forms from the simplest cytoplasmic-only to the more complicated ones involving nuclear dynamics, mimicking real cells. Such innate flexibility and robustness make it key to studying clock properties like tunability and stochasticity. Our results also highlight energy as an important regulator of cell cycles. We demonstrate a simple, powerful, and likely generalizable strategy of integrating strengths of single-cell approaches into conventional in vitro systems to study complex clock functions.
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spelling pubmed-59229722018-04-30 A robust and tunable mitotic oscillator in artificial cells Guan, Ye Li, Zhengda Wang, Shiyuan Barnes, Patrick M Liu, Xuwen Xu, Haotian Jin, Minjun Liu, Allen P Yang, Qiong eLife Biochemistry and Chemical Biology Single-cell analysis is pivotal to deciphering complex phenomena like heterogeneity, bistability, and asynchronous oscillations, where a population ensemble cannot represent individual behaviors. Bulk cell-free systems, despite having unique advantages of manipulation and characterization of biochemical networks, lack the essential single-cell information to understand a class of out-of-steady-state dynamics including cell cycles. Here, by encapsulating Xenopus egg extracts in water-in-oil microemulsions, we developed artificial cells that are adjustable in sizes and periods, sustain mitotic oscillations for over 30 cycles, and function in forms from the simplest cytoplasmic-only to the more complicated ones involving nuclear dynamics, mimicking real cells. Such innate flexibility and robustness make it key to studying clock properties like tunability and stochasticity. Our results also highlight energy as an important regulator of cell cycles. We demonstrate a simple, powerful, and likely generalizable strategy of integrating strengths of single-cell approaches into conventional in vitro systems to study complex clock functions. eLife Sciences Publications, Ltd 2018-04-05 /pmc/articles/PMC5922972/ /pubmed/29620527 http://dx.doi.org/10.7554/eLife.33549 Text en © 2018, Guan et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Biochemistry and Chemical Biology
Guan, Ye
Li, Zhengda
Wang, Shiyuan
Barnes, Patrick M
Liu, Xuwen
Xu, Haotian
Jin, Minjun
Liu, Allen P
Yang, Qiong
A robust and tunable mitotic oscillator in artificial cells
title A robust and tunable mitotic oscillator in artificial cells
title_full A robust and tunable mitotic oscillator in artificial cells
title_fullStr A robust and tunable mitotic oscillator in artificial cells
title_full_unstemmed A robust and tunable mitotic oscillator in artificial cells
title_short A robust and tunable mitotic oscillator in artificial cells
title_sort robust and tunable mitotic oscillator in artificial cells
topic Biochemistry and Chemical Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5922972/
https://www.ncbi.nlm.nih.gov/pubmed/29620527
http://dx.doi.org/10.7554/eLife.33549
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