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Interaction of the GntR-family transcription factor Sll1961 with thioredoxin in the cyanobacterium Synechocystis sp. PCC 6803

Changes in the redox state of the photosynthetic electron transport chain act as a signal to trigger acclimation responses to environmental cues and thioredoxin has been suggested to work as a key factor connecting the redox change with transcriptional regulation in the cyanobacterium Synechocystis...

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Autores principales: Kujirai, Junichi, Nanba, Sato, Kadowaki, Taro, Oka, Yoshiki, Nishiyama, Yoshitaka, Hayashi, Yuuki, Arai, Munehito, Hihara, Yukako
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923263/
https://www.ncbi.nlm.nih.gov/pubmed/29703909
http://dx.doi.org/10.1038/s41598-018-25077-5
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author Kujirai, Junichi
Nanba, Sato
Kadowaki, Taro
Oka, Yoshiki
Nishiyama, Yoshitaka
Hayashi, Yuuki
Arai, Munehito
Hihara, Yukako
author_facet Kujirai, Junichi
Nanba, Sato
Kadowaki, Taro
Oka, Yoshiki
Nishiyama, Yoshitaka
Hayashi, Yuuki
Arai, Munehito
Hihara, Yukako
author_sort Kujirai, Junichi
collection PubMed
description Changes in the redox state of the photosynthetic electron transport chain act as a signal to trigger acclimation responses to environmental cues and thioredoxin has been suggested to work as a key factor connecting the redox change with transcriptional regulation in the cyanobacterium Synechocystis sp. PCC 6803. We screened for redox-dependent transcription factors interacting with thioredoxin M (TrxM) and isolated the GntR-type transcription factor Sll1961 previously reported to be involved in acclimation responses of the photosynthetic machinery. Biochemical analyses using recombinant Sll1961 proteins of wild type and mutants of three cysteine residues, C124, C229 and C307, revealed that an intramolecular disulfide bond is formed between C229 and C307 under oxidizing conditions and TrxM can reduce it by attacking C307. Sll1961 exists in a dimeric form of about 80 kDa both under reducing and oxidizing conditions. C124 can form an intermolecular disulfide bond but it is not essential for dimerization. Based on these observations, tertiary structure models of the Sll1961 homodimer and the Sll1961-TrxM complex were constructed.
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spelling pubmed-59232632018-05-01 Interaction of the GntR-family transcription factor Sll1961 with thioredoxin in the cyanobacterium Synechocystis sp. PCC 6803 Kujirai, Junichi Nanba, Sato Kadowaki, Taro Oka, Yoshiki Nishiyama, Yoshitaka Hayashi, Yuuki Arai, Munehito Hihara, Yukako Sci Rep Article Changes in the redox state of the photosynthetic electron transport chain act as a signal to trigger acclimation responses to environmental cues and thioredoxin has been suggested to work as a key factor connecting the redox change with transcriptional regulation in the cyanobacterium Synechocystis sp. PCC 6803. We screened for redox-dependent transcription factors interacting with thioredoxin M (TrxM) and isolated the GntR-type transcription factor Sll1961 previously reported to be involved in acclimation responses of the photosynthetic machinery. Biochemical analyses using recombinant Sll1961 proteins of wild type and mutants of three cysteine residues, C124, C229 and C307, revealed that an intramolecular disulfide bond is formed between C229 and C307 under oxidizing conditions and TrxM can reduce it by attacking C307. Sll1961 exists in a dimeric form of about 80 kDa both under reducing and oxidizing conditions. C124 can form an intermolecular disulfide bond but it is not essential for dimerization. Based on these observations, tertiary structure models of the Sll1961 homodimer and the Sll1961-TrxM complex were constructed. Nature Publishing Group UK 2018-04-27 /pmc/articles/PMC5923263/ /pubmed/29703909 http://dx.doi.org/10.1038/s41598-018-25077-5 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kujirai, Junichi
Nanba, Sato
Kadowaki, Taro
Oka, Yoshiki
Nishiyama, Yoshitaka
Hayashi, Yuuki
Arai, Munehito
Hihara, Yukako
Interaction of the GntR-family transcription factor Sll1961 with thioredoxin in the cyanobacterium Synechocystis sp. PCC 6803
title Interaction of the GntR-family transcription factor Sll1961 with thioredoxin in the cyanobacterium Synechocystis sp. PCC 6803
title_full Interaction of the GntR-family transcription factor Sll1961 with thioredoxin in the cyanobacterium Synechocystis sp. PCC 6803
title_fullStr Interaction of the GntR-family transcription factor Sll1961 with thioredoxin in the cyanobacterium Synechocystis sp. PCC 6803
title_full_unstemmed Interaction of the GntR-family transcription factor Sll1961 with thioredoxin in the cyanobacterium Synechocystis sp. PCC 6803
title_short Interaction of the GntR-family transcription factor Sll1961 with thioredoxin in the cyanobacterium Synechocystis sp. PCC 6803
title_sort interaction of the gntr-family transcription factor sll1961 with thioredoxin in the cyanobacterium synechocystis sp. pcc 6803
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923263/
https://www.ncbi.nlm.nih.gov/pubmed/29703909
http://dx.doi.org/10.1038/s41598-018-25077-5
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