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A Plasma Biomarker Panel of Four MicroRNAs for the Diagnosis of Prostate Cancer

Prostate cancer is diagnosed in over 1 million men every year globally, yet current diagnostic modalities are inadequate for identification of significant cancer and more reliable early diagnostic biomarkers are necessary for improved clinical management of prostate cancer patients. MicroRNAs (miRNA...

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Autores principales: Matin, Farhana, Jeet, Varinder, Moya, Leire, Selth, Luke A., Chambers, Suzanne, Clements, Judith A., Batra, Jyotsna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923293/
https://www.ncbi.nlm.nih.gov/pubmed/29703916
http://dx.doi.org/10.1038/s41598-018-24424-w
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author Matin, Farhana
Jeet, Varinder
Moya, Leire
Selth, Luke A.
Chambers, Suzanne
Clements, Judith A.
Batra, Jyotsna
author_facet Matin, Farhana
Jeet, Varinder
Moya, Leire
Selth, Luke A.
Chambers, Suzanne
Clements, Judith A.
Batra, Jyotsna
author_sort Matin, Farhana
collection PubMed
description Prostate cancer is diagnosed in over 1 million men every year globally, yet current diagnostic modalities are inadequate for identification of significant cancer and more reliable early diagnostic biomarkers are necessary for improved clinical management of prostate cancer patients. MicroRNAs (miRNAs) modulate important cellular processes/pathways contributing to cancer and are stably present in body fluids. In this study we profiled 372 cancer-associated miRNAs in plasma collected before (~60% patients) and after/during commencement of treatment (~40% patients), from age-matched prostate cancer patients and healthy controls, and observed elevated levels of 4 miRNAs - miR-4289, miR-326, miR-152-3p and miR-98-5p, which were validated in an independent cohort. The miRNA panel was able to differentiate between prostate cancer patients and controls (AUC = 0.88). Analysis of published miRNA transcriptomic data from clinical samples demonstrated low expression of miR-152-3p in tumour compared to adjacent non-malignant tissues. Overexpression of miR-152-3p increased proliferation and migration of prostate cancer cells, suggesting a role for this miRNA in prostate cancer pathogenesis, a concept that was supported by pathway analysis of predicted miR-152-3p target genes. In summary, a four miRNA panel, including miR-152-3p which likely targets genes with key roles in prostate cancer pathogenesis, has the potential to improve early prostate cancer diagnosis.
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spelling pubmed-59232932018-05-01 A Plasma Biomarker Panel of Four MicroRNAs for the Diagnosis of Prostate Cancer Matin, Farhana Jeet, Varinder Moya, Leire Selth, Luke A. Chambers, Suzanne Clements, Judith A. Batra, Jyotsna Sci Rep Article Prostate cancer is diagnosed in over 1 million men every year globally, yet current diagnostic modalities are inadequate for identification of significant cancer and more reliable early diagnostic biomarkers are necessary for improved clinical management of prostate cancer patients. MicroRNAs (miRNAs) modulate important cellular processes/pathways contributing to cancer and are stably present in body fluids. In this study we profiled 372 cancer-associated miRNAs in plasma collected before (~60% patients) and after/during commencement of treatment (~40% patients), from age-matched prostate cancer patients and healthy controls, and observed elevated levels of 4 miRNAs - miR-4289, miR-326, miR-152-3p and miR-98-5p, which were validated in an independent cohort. The miRNA panel was able to differentiate between prostate cancer patients and controls (AUC = 0.88). Analysis of published miRNA transcriptomic data from clinical samples demonstrated low expression of miR-152-3p in tumour compared to adjacent non-malignant tissues. Overexpression of miR-152-3p increased proliferation and migration of prostate cancer cells, suggesting a role for this miRNA in prostate cancer pathogenesis, a concept that was supported by pathway analysis of predicted miR-152-3p target genes. In summary, a four miRNA panel, including miR-152-3p which likely targets genes with key roles in prostate cancer pathogenesis, has the potential to improve early prostate cancer diagnosis. Nature Publishing Group UK 2018-04-27 /pmc/articles/PMC5923293/ /pubmed/29703916 http://dx.doi.org/10.1038/s41598-018-24424-w Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Matin, Farhana
Jeet, Varinder
Moya, Leire
Selth, Luke A.
Chambers, Suzanne
Clements, Judith A.
Batra, Jyotsna
A Plasma Biomarker Panel of Four MicroRNAs for the Diagnosis of Prostate Cancer
title A Plasma Biomarker Panel of Four MicroRNAs for the Diagnosis of Prostate Cancer
title_full A Plasma Biomarker Panel of Four MicroRNAs for the Diagnosis of Prostate Cancer
title_fullStr A Plasma Biomarker Panel of Four MicroRNAs for the Diagnosis of Prostate Cancer
title_full_unstemmed A Plasma Biomarker Panel of Four MicroRNAs for the Diagnosis of Prostate Cancer
title_short A Plasma Biomarker Panel of Four MicroRNAs for the Diagnosis of Prostate Cancer
title_sort plasma biomarker panel of four micrornas for the diagnosis of prostate cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923293/
https://www.ncbi.nlm.nih.gov/pubmed/29703916
http://dx.doi.org/10.1038/s41598-018-24424-w
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