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OTA Prevention and Detoxification by Actinobacterial Strains and Activated Carbon Fibers: Preliminary Results

Ochratoxin A (OTA) is a mycotoxin produced by several species of Aspergillus and Penicillium that contaminate food and feed raw materials. To reduce OTA contamination, we first tested in vitro, actinobacterial strains as potential biocontrol agents and afterward, through a physical decontamination m...

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Autores principales: El Khoury, Rhoda, Choque, Elodie, El Khoury, Anthony, Snini, Selma P., Cairns, Robbie, Andriantsiferana, Caroline, Mathieu, Florence
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923303/
https://www.ncbi.nlm.nih.gov/pubmed/29587362
http://dx.doi.org/10.3390/toxins10040137
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author El Khoury, Rhoda
Choque, Elodie
El Khoury, Anthony
Snini, Selma P.
Cairns, Robbie
Andriantsiferana, Caroline
Mathieu, Florence
author_facet El Khoury, Rhoda
Choque, Elodie
El Khoury, Anthony
Snini, Selma P.
Cairns, Robbie
Andriantsiferana, Caroline
Mathieu, Florence
author_sort El Khoury, Rhoda
collection PubMed
description Ochratoxin A (OTA) is a mycotoxin produced by several species of Aspergillus and Penicillium that contaminate food and feed raw materials. To reduce OTA contamination, we first tested in vitro, actinobacterial strains as potential biocontrol agents and afterward, through a physical decontamination method using activated carbon fibers (ACFs). Actinobacterial strains were screened for their ability to reduce OTA in solid co-culture with A. carbonarius, which is the major OTA-producing species in European vineyards. Four strains showed a high affinity for removing OTA (67%–83%) with no significant effect on fungal growth (<20%). The mechanism of action was first studied by analyzing the expression of OTA cluster genes (acOTApks, acOTAnrps, acOTAhal) by RT-qPCR showing a drastic reduction in all genes (7–15 times). Second, the ability of these strains to degrade OTA was assessed in vitro on ISP2 solid medium supplemented with OTA (100 µg/L). Two strains reduced OTA to undetectable levels. As for the physical method, high adsorption rates were obtained for ACFs at 0.8 g/L with a 50% adsorption of OTA in red wine by AC15 and 52% in grape juice by AC20 within 24 h. These promising methods could be complementarily applied toward reducing OTA contamination in food chains, which promotes food safety and quality.
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spelling pubmed-59233032018-05-03 OTA Prevention and Detoxification by Actinobacterial Strains and Activated Carbon Fibers: Preliminary Results El Khoury, Rhoda Choque, Elodie El Khoury, Anthony Snini, Selma P. Cairns, Robbie Andriantsiferana, Caroline Mathieu, Florence Toxins (Basel) Article Ochratoxin A (OTA) is a mycotoxin produced by several species of Aspergillus and Penicillium that contaminate food and feed raw materials. To reduce OTA contamination, we first tested in vitro, actinobacterial strains as potential biocontrol agents and afterward, through a physical decontamination method using activated carbon fibers (ACFs). Actinobacterial strains were screened for their ability to reduce OTA in solid co-culture with A. carbonarius, which is the major OTA-producing species in European vineyards. Four strains showed a high affinity for removing OTA (67%–83%) with no significant effect on fungal growth (<20%). The mechanism of action was first studied by analyzing the expression of OTA cluster genes (acOTApks, acOTAnrps, acOTAhal) by RT-qPCR showing a drastic reduction in all genes (7–15 times). Second, the ability of these strains to degrade OTA was assessed in vitro on ISP2 solid medium supplemented with OTA (100 µg/L). Two strains reduced OTA to undetectable levels. As for the physical method, high adsorption rates were obtained for ACFs at 0.8 g/L with a 50% adsorption of OTA in red wine by AC15 and 52% in grape juice by AC20 within 24 h. These promising methods could be complementarily applied toward reducing OTA contamination in food chains, which promotes food safety and quality. MDPI 2018-03-24 /pmc/articles/PMC5923303/ /pubmed/29587362 http://dx.doi.org/10.3390/toxins10040137 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
El Khoury, Rhoda
Choque, Elodie
El Khoury, Anthony
Snini, Selma P.
Cairns, Robbie
Andriantsiferana, Caroline
Mathieu, Florence
OTA Prevention and Detoxification by Actinobacterial Strains and Activated Carbon Fibers: Preliminary Results
title OTA Prevention and Detoxification by Actinobacterial Strains and Activated Carbon Fibers: Preliminary Results
title_full OTA Prevention and Detoxification by Actinobacterial Strains and Activated Carbon Fibers: Preliminary Results
title_fullStr OTA Prevention and Detoxification by Actinobacterial Strains and Activated Carbon Fibers: Preliminary Results
title_full_unstemmed OTA Prevention and Detoxification by Actinobacterial Strains and Activated Carbon Fibers: Preliminary Results
title_short OTA Prevention and Detoxification by Actinobacterial Strains and Activated Carbon Fibers: Preliminary Results
title_sort ota prevention and detoxification by actinobacterial strains and activated carbon fibers: preliminary results
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923303/
https://www.ncbi.nlm.nih.gov/pubmed/29587362
http://dx.doi.org/10.3390/toxins10040137
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