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Bovine Peripheral Blood Mononuclear Cells Are More Sensitive to Deoxynivalenol Than Those Derived from Poultry and Swine

Deoxynivalenol (DON) is one of the most prevalent mycotoxins, contaminating cereals and cereal-derived products. Its derivative deepoxy-deoxynivalenol (DOM-1) is produced by certain bacteria, which either occur naturally or are supplemented in feed additive. DON-induced impairments in protein synthe...

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Autores principales: Novak, Barbara, Vatzia, Eleni, Springler, Alexandra, Pierron, Alix, Gerner, Wilhelm, Reisinger, Nicole, Hessenberger, Sabine, Schatzmayr, Gerd, Mayer, Elisabeth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923318/
https://www.ncbi.nlm.nih.gov/pubmed/29641442
http://dx.doi.org/10.3390/toxins10040152
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author Novak, Barbara
Vatzia, Eleni
Springler, Alexandra
Pierron, Alix
Gerner, Wilhelm
Reisinger, Nicole
Hessenberger, Sabine
Schatzmayr, Gerd
Mayer, Elisabeth
author_facet Novak, Barbara
Vatzia, Eleni
Springler, Alexandra
Pierron, Alix
Gerner, Wilhelm
Reisinger, Nicole
Hessenberger, Sabine
Schatzmayr, Gerd
Mayer, Elisabeth
author_sort Novak, Barbara
collection PubMed
description Deoxynivalenol (DON) is one of the most prevalent mycotoxins, contaminating cereals and cereal-derived products. Its derivative deepoxy-deoxynivalenol (DOM-1) is produced by certain bacteria, which either occur naturally or are supplemented in feed additive. DON-induced impairments in protein synthesis are particularly problematic for highly proliferating immune cells. This study provides the first comparison of the effects of DON and DOM-1 on the concanavalin A-induced proliferation of porcine, chicken, and bovine peripheral blood mononuclear cells (PBMCs). Therefore, isolated PBMCs were treated with DON (0.01–3.37 µM) and DOM-1 (1.39–357 µM) separately, and proliferation was measured using a bromodeoxyuridine (BrdU) assay. Although pigs are considered highly sensitive to DON, the present study revealed a substantially higher sensitivity of bovine (IC50 = 0.314 µM) PBMCs compared to chicken (IC50 = 0.691 µM) and porcine (IC50 = 0.693 µM) PBMCs. Analyses on the proliferation of bovine T-cell subsets showed that all major subsets, namely, CD4(+), CD8β(+), and γδ T cells, were affected to a similar extent. In contrast, DOM-1 did not affect bovine PBMCs, but reduced the proliferation of chicken and porcine PBMCs at the highest tested concentration (357 µM). Results confirm the necessity of feed additives containing DON-to-DOM-1-transforming bacteria and highlights species-specific differences in the DON sensitivity of immune cells.
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spelling pubmed-59233182018-05-03 Bovine Peripheral Blood Mononuclear Cells Are More Sensitive to Deoxynivalenol Than Those Derived from Poultry and Swine Novak, Barbara Vatzia, Eleni Springler, Alexandra Pierron, Alix Gerner, Wilhelm Reisinger, Nicole Hessenberger, Sabine Schatzmayr, Gerd Mayer, Elisabeth Toxins (Basel) Article Deoxynivalenol (DON) is one of the most prevalent mycotoxins, contaminating cereals and cereal-derived products. Its derivative deepoxy-deoxynivalenol (DOM-1) is produced by certain bacteria, which either occur naturally or are supplemented in feed additive. DON-induced impairments in protein synthesis are particularly problematic for highly proliferating immune cells. This study provides the first comparison of the effects of DON and DOM-1 on the concanavalin A-induced proliferation of porcine, chicken, and bovine peripheral blood mononuclear cells (PBMCs). Therefore, isolated PBMCs were treated with DON (0.01–3.37 µM) and DOM-1 (1.39–357 µM) separately, and proliferation was measured using a bromodeoxyuridine (BrdU) assay. Although pigs are considered highly sensitive to DON, the present study revealed a substantially higher sensitivity of bovine (IC50 = 0.314 µM) PBMCs compared to chicken (IC50 = 0.691 µM) and porcine (IC50 = 0.693 µM) PBMCs. Analyses on the proliferation of bovine T-cell subsets showed that all major subsets, namely, CD4(+), CD8β(+), and γδ T cells, were affected to a similar extent. In contrast, DOM-1 did not affect bovine PBMCs, but reduced the proliferation of chicken and porcine PBMCs at the highest tested concentration (357 µM). Results confirm the necessity of feed additives containing DON-to-DOM-1-transforming bacteria and highlights species-specific differences in the DON sensitivity of immune cells. MDPI 2018-04-11 /pmc/articles/PMC5923318/ /pubmed/29641442 http://dx.doi.org/10.3390/toxins10040152 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Novak, Barbara
Vatzia, Eleni
Springler, Alexandra
Pierron, Alix
Gerner, Wilhelm
Reisinger, Nicole
Hessenberger, Sabine
Schatzmayr, Gerd
Mayer, Elisabeth
Bovine Peripheral Blood Mononuclear Cells Are More Sensitive to Deoxynivalenol Than Those Derived from Poultry and Swine
title Bovine Peripheral Blood Mononuclear Cells Are More Sensitive to Deoxynivalenol Than Those Derived from Poultry and Swine
title_full Bovine Peripheral Blood Mononuclear Cells Are More Sensitive to Deoxynivalenol Than Those Derived from Poultry and Swine
title_fullStr Bovine Peripheral Blood Mononuclear Cells Are More Sensitive to Deoxynivalenol Than Those Derived from Poultry and Swine
title_full_unstemmed Bovine Peripheral Blood Mononuclear Cells Are More Sensitive to Deoxynivalenol Than Those Derived from Poultry and Swine
title_short Bovine Peripheral Blood Mononuclear Cells Are More Sensitive to Deoxynivalenol Than Those Derived from Poultry and Swine
title_sort bovine peripheral blood mononuclear cells are more sensitive to deoxynivalenol than those derived from poultry and swine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923318/
https://www.ncbi.nlm.nih.gov/pubmed/29641442
http://dx.doi.org/10.3390/toxins10040152
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