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A Low Molecular Weight Protein from the Sea Anemone Anemonia viridis with an Anti-Angiogenic Activity

Sea anemones are a remarkable source of active principles due to a decentralized venom system. New blood vessel growth or angiogenesis is a very promising target against cancer, but the few available antiangiogenic compounds have limited efficacy. In this study, a protein fraction, purified from ten...

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Detalles Bibliográficos
Autores principales: Loret, Erwann P., Luis, José, Nuccio, Christopher, Villard, Claude, Mansuelle, Pascal, Lebrun, Régine, Villard, Pierre Henri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923421/
https://www.ncbi.nlm.nih.gov/pubmed/29671760
http://dx.doi.org/10.3390/md16040134
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author Loret, Erwann P.
Luis, José
Nuccio, Christopher
Villard, Claude
Mansuelle, Pascal
Lebrun, Régine
Villard, Pierre Henri
author_facet Loret, Erwann P.
Luis, José
Nuccio, Christopher
Villard, Claude
Mansuelle, Pascal
Lebrun, Régine
Villard, Pierre Henri
author_sort Loret, Erwann P.
collection PubMed
description Sea anemones are a remarkable source of active principles due to a decentralized venom system. New blood vessel growth or angiogenesis is a very promising target against cancer, but the few available antiangiogenic compounds have limited efficacy. In this study, a protein fraction, purified from tentacles of Anemonia viridis, was able to limit endothelial cells proliferation and angiogenesis at low concentration (14 nM). Protein sequences were determined with Edman degradation and mass spectrometry in source decay and revealed homologies with Blood Depressing Substance (BDS) sea anemones. The presence of a two-turn alpha helix observed with circular dichroism and a trypsin activity inhibition suggested that the active principle could be a Kunitz-type inhibitor, which may interact with an integrin due to an Arginine Glycin Aspartate (RGD) motif. Molecular modeling showed that this RGD motif was well exposed to solvent. This active principle could improve antiangiogenic therapy from existing antiangiogenic compounds binding on the Vascular Endothelial Growth Factor (VEGF).
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spelling pubmed-59234212018-05-03 A Low Molecular Weight Protein from the Sea Anemone Anemonia viridis with an Anti-Angiogenic Activity Loret, Erwann P. Luis, José Nuccio, Christopher Villard, Claude Mansuelle, Pascal Lebrun, Régine Villard, Pierre Henri Mar Drugs Article Sea anemones are a remarkable source of active principles due to a decentralized venom system. New blood vessel growth or angiogenesis is a very promising target against cancer, but the few available antiangiogenic compounds have limited efficacy. In this study, a protein fraction, purified from tentacles of Anemonia viridis, was able to limit endothelial cells proliferation and angiogenesis at low concentration (14 nM). Protein sequences were determined with Edman degradation and mass spectrometry in source decay and revealed homologies with Blood Depressing Substance (BDS) sea anemones. The presence of a two-turn alpha helix observed with circular dichroism and a trypsin activity inhibition suggested that the active principle could be a Kunitz-type inhibitor, which may interact with an integrin due to an Arginine Glycin Aspartate (RGD) motif. Molecular modeling showed that this RGD motif was well exposed to solvent. This active principle could improve antiangiogenic therapy from existing antiangiogenic compounds binding on the Vascular Endothelial Growth Factor (VEGF). MDPI 2018-04-19 /pmc/articles/PMC5923421/ /pubmed/29671760 http://dx.doi.org/10.3390/md16040134 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Loret, Erwann P.
Luis, José
Nuccio, Christopher
Villard, Claude
Mansuelle, Pascal
Lebrun, Régine
Villard, Pierre Henri
A Low Molecular Weight Protein from the Sea Anemone Anemonia viridis with an Anti-Angiogenic Activity
title A Low Molecular Weight Protein from the Sea Anemone Anemonia viridis with an Anti-Angiogenic Activity
title_full A Low Molecular Weight Protein from the Sea Anemone Anemonia viridis with an Anti-Angiogenic Activity
title_fullStr A Low Molecular Weight Protein from the Sea Anemone Anemonia viridis with an Anti-Angiogenic Activity
title_full_unstemmed A Low Molecular Weight Protein from the Sea Anemone Anemonia viridis with an Anti-Angiogenic Activity
title_short A Low Molecular Weight Protein from the Sea Anemone Anemonia viridis with an Anti-Angiogenic Activity
title_sort low molecular weight protein from the sea anemone anemonia viridis with an anti-angiogenic activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923421/
https://www.ncbi.nlm.nih.gov/pubmed/29671760
http://dx.doi.org/10.3390/md16040134
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