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Bioactive Compounds from Posidonia oceanica (L.) Delile Impair Malignant Cell Migration through Autophagy Modulation

Posidonia oceanica (L.) Delile is a marine plant with interesting biological properties potentially ascribed to the synergistic combination of bioactive compounds. Our previously described extract, obtained from the leaves of P. oceanica, showed the ability to impair HT1080 cell migration by targeti...

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Autores principales: Leri, Manuela, Ramazzotti, Matteo, Vasarri, Marzia, Peri, Sara, Barletta, Emanuela, Pretti, Carlo, Degl’Innocenti, Donatella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923424/
https://www.ncbi.nlm.nih.gov/pubmed/29690502
http://dx.doi.org/10.3390/md16040137
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author Leri, Manuela
Ramazzotti, Matteo
Vasarri, Marzia
Peri, Sara
Barletta, Emanuela
Pretti, Carlo
Degl’Innocenti, Donatella
author_facet Leri, Manuela
Ramazzotti, Matteo
Vasarri, Marzia
Peri, Sara
Barletta, Emanuela
Pretti, Carlo
Degl’Innocenti, Donatella
author_sort Leri, Manuela
collection PubMed
description Posidonia oceanica (L.) Delile is a marine plant with interesting biological properties potentially ascribed to the synergistic combination of bioactive compounds. Our previously described extract, obtained from the leaves of P. oceanica, showed the ability to impair HT1080 cell migration by targeting both expression and activity of gelatinases. Commonly, the lack of knowledge about the mechanism of action of phytocomplexes may be an obstacle regarding their therapeutic use and development. The aim of this study was to gain insight into the molecular signaling through which such bioactive compounds impact on malignant cell migration and gelatinolytic activity. The increase in autophagic vacuoles detected by confocal microscopy suggested an enhancement of autophagy in a time and dose dependent manner. This autophagy activation was further confirmed by monitoring pivotal markers of autophagy signaling as well as by evidencing an increase in IGF-1R accumulation on cell membranes. Taken together, our results confirm that the P. oceanica phytocomplex is a promising reservoir of potent and cell safe molecules able to defend against malignancies and other diseases in which gelatinases play a major role in progression. In conclusion, the attractive properties of this phytocomplex may be of industrial interest in regard to the development of novel health-promoting and pharmacological products for the treatment or prevention of several diseases.
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spelling pubmed-59234242018-05-03 Bioactive Compounds from Posidonia oceanica (L.) Delile Impair Malignant Cell Migration through Autophagy Modulation Leri, Manuela Ramazzotti, Matteo Vasarri, Marzia Peri, Sara Barletta, Emanuela Pretti, Carlo Degl’Innocenti, Donatella Mar Drugs Article Posidonia oceanica (L.) Delile is a marine plant with interesting biological properties potentially ascribed to the synergistic combination of bioactive compounds. Our previously described extract, obtained from the leaves of P. oceanica, showed the ability to impair HT1080 cell migration by targeting both expression and activity of gelatinases. Commonly, the lack of knowledge about the mechanism of action of phytocomplexes may be an obstacle regarding their therapeutic use and development. The aim of this study was to gain insight into the molecular signaling through which such bioactive compounds impact on malignant cell migration and gelatinolytic activity. The increase in autophagic vacuoles detected by confocal microscopy suggested an enhancement of autophagy in a time and dose dependent manner. This autophagy activation was further confirmed by monitoring pivotal markers of autophagy signaling as well as by evidencing an increase in IGF-1R accumulation on cell membranes. Taken together, our results confirm that the P. oceanica phytocomplex is a promising reservoir of potent and cell safe molecules able to defend against malignancies and other diseases in which gelatinases play a major role in progression. In conclusion, the attractive properties of this phytocomplex may be of industrial interest in regard to the development of novel health-promoting and pharmacological products for the treatment or prevention of several diseases. MDPI 2018-04-21 /pmc/articles/PMC5923424/ /pubmed/29690502 http://dx.doi.org/10.3390/md16040137 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Leri, Manuela
Ramazzotti, Matteo
Vasarri, Marzia
Peri, Sara
Barletta, Emanuela
Pretti, Carlo
Degl’Innocenti, Donatella
Bioactive Compounds from Posidonia oceanica (L.) Delile Impair Malignant Cell Migration through Autophagy Modulation
title Bioactive Compounds from Posidonia oceanica (L.) Delile Impair Malignant Cell Migration through Autophagy Modulation
title_full Bioactive Compounds from Posidonia oceanica (L.) Delile Impair Malignant Cell Migration through Autophagy Modulation
title_fullStr Bioactive Compounds from Posidonia oceanica (L.) Delile Impair Malignant Cell Migration through Autophagy Modulation
title_full_unstemmed Bioactive Compounds from Posidonia oceanica (L.) Delile Impair Malignant Cell Migration through Autophagy Modulation
title_short Bioactive Compounds from Posidonia oceanica (L.) Delile Impair Malignant Cell Migration through Autophagy Modulation
title_sort bioactive compounds from posidonia oceanica (l.) delile impair malignant cell migration through autophagy modulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923424/
https://www.ncbi.nlm.nih.gov/pubmed/29690502
http://dx.doi.org/10.3390/md16040137
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