Photoactivatable drugs for nicotinic optopharmacology

Photoactivatable (‘caged’) pharmacological agents have revolutionized neuroscience but the palette of available compounds is limited. We describe a general method for caging tertiary amines using a stable quaternary ammonium linkage that elicits a red-shift in activation wavelength. We prepared a ph...

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Detalles Bibliográficos
Autores principales: Banala, Sambashiva, Arvin, Matthew C., Bannon, Nicolas M., Jin, Xiao-Tao, Macklin, John J., Wang, Yong, Peng, Can, Zhao, Guiqing, Marshall, John J., Gee, Kyle R., Wokosin, David L., Kim, Veronica J., McIntosh, J. Michael, Contractor, Anis, Lester, Henry A., Kozorovitskiy, Yevgenia, Drenan, Ryan M., Lavis, Luke D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923430/
https://www.ncbi.nlm.nih.gov/pubmed/29578537
http://dx.doi.org/10.1038/nmeth.4637
Descripción
Sumario:Photoactivatable (‘caged’) pharmacological agents have revolutionized neuroscience but the palette of available compounds is limited. We describe a general method for caging tertiary amines using a stable quaternary ammonium linkage that elicits a red-shift in activation wavelength. We prepared a photoactivatable nicotine (PA-Nic), uncagable via 1- or 2-photon excitation, that is useful for optopharmacology experiments to study nicotinic acetylcholine receptors (nAChRs) in different experimental preparations and spatiotemporal scales.

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