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Photoactivatable drugs for nicotinic optopharmacology
Photoactivatable (‘caged’) pharmacological agents have revolutionized neuroscience but the palette of available compounds is limited. We describe a general method for caging tertiary amines using a stable quaternary ammonium linkage that elicits a red-shift in activation wavelength. We prepared a ph...
| Autores principales: | , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923430/ https://www.ncbi.nlm.nih.gov/pubmed/29578537 http://dx.doi.org/10.1038/nmeth.4637 |
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| author | Banala, Sambashiva Arvin, Matthew C. Bannon, Nicolas M. Jin, Xiao-Tao Macklin, John J. Wang, Yong Peng, Can Zhao, Guiqing Marshall, John J. Gee, Kyle R. Wokosin, David L. Kim, Veronica J. McIntosh, J. Michael Contractor, Anis Lester, Henry A. Kozorovitskiy, Yevgenia Drenan, Ryan M. Lavis, Luke D. |
| author_facet | Banala, Sambashiva Arvin, Matthew C. Bannon, Nicolas M. Jin, Xiao-Tao Macklin, John J. Wang, Yong Peng, Can Zhao, Guiqing Marshall, John J. Gee, Kyle R. Wokosin, David L. Kim, Veronica J. McIntosh, J. Michael Contractor, Anis Lester, Henry A. Kozorovitskiy, Yevgenia Drenan, Ryan M. Lavis, Luke D. |
| author_sort | Banala, Sambashiva |
| collection | PubMed |
| description | Photoactivatable (‘caged’) pharmacological agents have revolutionized neuroscience but the palette of available compounds is limited. We describe a general method for caging tertiary amines using a stable quaternary ammonium linkage that elicits a red-shift in activation wavelength. We prepared a photoactivatable nicotine (PA-Nic), uncagable via 1- or 2-photon excitation, that is useful for optopharmacology experiments to study nicotinic acetylcholine receptors (nAChRs) in different experimental preparations and spatiotemporal scales. |
| format | Online Article Text |
| id | pubmed-5923430 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2018 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-59234302018-09-26 Photoactivatable drugs for nicotinic optopharmacology Banala, Sambashiva Arvin, Matthew C. Bannon, Nicolas M. Jin, Xiao-Tao Macklin, John J. Wang, Yong Peng, Can Zhao, Guiqing Marshall, John J. Gee, Kyle R. Wokosin, David L. Kim, Veronica J. McIntosh, J. Michael Contractor, Anis Lester, Henry A. Kozorovitskiy, Yevgenia Drenan, Ryan M. Lavis, Luke D. Nat Methods Article Photoactivatable (‘caged’) pharmacological agents have revolutionized neuroscience but the palette of available compounds is limited. We describe a general method for caging tertiary amines using a stable quaternary ammonium linkage that elicits a red-shift in activation wavelength. We prepared a photoactivatable nicotine (PA-Nic), uncagable via 1- or 2-photon excitation, that is useful for optopharmacology experiments to study nicotinic acetylcholine receptors (nAChRs) in different experimental preparations and spatiotemporal scales. 2018-03-26 2018-05 /pmc/articles/PMC5923430/ /pubmed/29578537 http://dx.doi.org/10.1038/nmeth.4637 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article Banala, Sambashiva Arvin, Matthew C. Bannon, Nicolas M. Jin, Xiao-Tao Macklin, John J. Wang, Yong Peng, Can Zhao, Guiqing Marshall, John J. Gee, Kyle R. Wokosin, David L. Kim, Veronica J. McIntosh, J. Michael Contractor, Anis Lester, Henry A. Kozorovitskiy, Yevgenia Drenan, Ryan M. Lavis, Luke D. Photoactivatable drugs for nicotinic optopharmacology |
| title | Photoactivatable drugs for nicotinic optopharmacology |
| title_full | Photoactivatable drugs for nicotinic optopharmacology |
| title_fullStr | Photoactivatable drugs for nicotinic optopharmacology |
| title_full_unstemmed | Photoactivatable drugs for nicotinic optopharmacology |
| title_short | Photoactivatable drugs for nicotinic optopharmacology |
| title_sort | photoactivatable drugs for nicotinic optopharmacology |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923430/ https://www.ncbi.nlm.nih.gov/pubmed/29578537 http://dx.doi.org/10.1038/nmeth.4637 |
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