Cargando…

Molecular Characterization of a Novel Species of Capillovirus from Japanese Apricot (Prunus mume)

With the increased use of high-throughput sequencing methods, new viruses infecting Prunus spp. are being discovered and characterized, especially in the family Betaflexiviridae. Double-stranded RNAs from symptomatic leaves of a Japanese apricot (Prunus mume) tree from Japan were purified and analyz...

Descripción completa

Detalles Bibliográficos
Autores principales: Marais, Armelle, Faure, Chantal, Theil, Sébastien, Candresse, Thierry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923438/
https://www.ncbi.nlm.nih.gov/pubmed/29570605
http://dx.doi.org/10.3390/v10040144
_version_ 1783318341406949376
author Marais, Armelle
Faure, Chantal
Theil, Sébastien
Candresse, Thierry
author_facet Marais, Armelle
Faure, Chantal
Theil, Sébastien
Candresse, Thierry
author_sort Marais, Armelle
collection PubMed
description With the increased use of high-throughput sequencing methods, new viruses infecting Prunus spp. are being discovered and characterized, especially in the family Betaflexiviridae. Double-stranded RNAs from symptomatic leaves of a Japanese apricot (Prunus mume) tree from Japan were purified and analyzed by Illumina sequencing. Blast comparisons of reconstructed contigs showed that the P. mume sample was infected by a putative novel virus with homologies to Cherry virus A (CVA) and to the newly described Currant virus A (CuVA), both members of genus Capillovirus. Completion of the genome showed the new agent to have a genomic organization typical of capilloviruses, with two overlapping open reading frames encoding a large replication-associated protein fused to the coat protein (CP), and a putative movement protein (MP). This virus shares only, respectively, 63.2% and 62.7% CP amino acid identity with the most closely related viruses, CVA and CuVA. Considering the species demarcation criteria in the family and phylogenetic analyses, this virus should be considered as representing a new viral species in the genus Capillovirus, for which the name of Mume virus A is proposed.
format Online
Article
Text
id pubmed-5923438
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-59234382018-05-03 Molecular Characterization of a Novel Species of Capillovirus from Japanese Apricot (Prunus mume) Marais, Armelle Faure, Chantal Theil, Sébastien Candresse, Thierry Viruses Communication With the increased use of high-throughput sequencing methods, new viruses infecting Prunus spp. are being discovered and characterized, especially in the family Betaflexiviridae. Double-stranded RNAs from symptomatic leaves of a Japanese apricot (Prunus mume) tree from Japan were purified and analyzed by Illumina sequencing. Blast comparisons of reconstructed contigs showed that the P. mume sample was infected by a putative novel virus with homologies to Cherry virus A (CVA) and to the newly described Currant virus A (CuVA), both members of genus Capillovirus. Completion of the genome showed the new agent to have a genomic organization typical of capilloviruses, with two overlapping open reading frames encoding a large replication-associated protein fused to the coat protein (CP), and a putative movement protein (MP). This virus shares only, respectively, 63.2% and 62.7% CP amino acid identity with the most closely related viruses, CVA and CuVA. Considering the species demarcation criteria in the family and phylogenetic analyses, this virus should be considered as representing a new viral species in the genus Capillovirus, for which the name of Mume virus A is proposed. MDPI 2018-03-23 /pmc/articles/PMC5923438/ /pubmed/29570605 http://dx.doi.org/10.3390/v10040144 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Marais, Armelle
Faure, Chantal
Theil, Sébastien
Candresse, Thierry
Molecular Characterization of a Novel Species of Capillovirus from Japanese Apricot (Prunus mume)
title Molecular Characterization of a Novel Species of Capillovirus from Japanese Apricot (Prunus mume)
title_full Molecular Characterization of a Novel Species of Capillovirus from Japanese Apricot (Prunus mume)
title_fullStr Molecular Characterization of a Novel Species of Capillovirus from Japanese Apricot (Prunus mume)
title_full_unstemmed Molecular Characterization of a Novel Species of Capillovirus from Japanese Apricot (Prunus mume)
title_short Molecular Characterization of a Novel Species of Capillovirus from Japanese Apricot (Prunus mume)
title_sort molecular characterization of a novel species of capillovirus from japanese apricot (prunus mume)
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923438/
https://www.ncbi.nlm.nih.gov/pubmed/29570605
http://dx.doi.org/10.3390/v10040144
work_keys_str_mv AT maraisarmelle molecularcharacterizationofanovelspeciesofcapillovirusfromjapaneseapricotprunusmume
AT faurechantal molecularcharacterizationofanovelspeciesofcapillovirusfromjapaneseapricotprunusmume
AT theilsebastien molecularcharacterizationofanovelspeciesofcapillovirusfromjapaneseapricotprunusmume
AT candressethierry molecularcharacterizationofanovelspeciesofcapillovirusfromjapaneseapricotprunusmume