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Molecular Characterization of a Novel Species of Capillovirus from Japanese Apricot (Prunus mume)
With the increased use of high-throughput sequencing methods, new viruses infecting Prunus spp. are being discovered and characterized, especially in the family Betaflexiviridae. Double-stranded RNAs from symptomatic leaves of a Japanese apricot (Prunus mume) tree from Japan were purified and analyz...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923438/ https://www.ncbi.nlm.nih.gov/pubmed/29570605 http://dx.doi.org/10.3390/v10040144 |
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author | Marais, Armelle Faure, Chantal Theil, Sébastien Candresse, Thierry |
author_facet | Marais, Armelle Faure, Chantal Theil, Sébastien Candresse, Thierry |
author_sort | Marais, Armelle |
collection | PubMed |
description | With the increased use of high-throughput sequencing methods, new viruses infecting Prunus spp. are being discovered and characterized, especially in the family Betaflexiviridae. Double-stranded RNAs from symptomatic leaves of a Japanese apricot (Prunus mume) tree from Japan were purified and analyzed by Illumina sequencing. Blast comparisons of reconstructed contigs showed that the P. mume sample was infected by a putative novel virus with homologies to Cherry virus A (CVA) and to the newly described Currant virus A (CuVA), both members of genus Capillovirus. Completion of the genome showed the new agent to have a genomic organization typical of capilloviruses, with two overlapping open reading frames encoding a large replication-associated protein fused to the coat protein (CP), and a putative movement protein (MP). This virus shares only, respectively, 63.2% and 62.7% CP amino acid identity with the most closely related viruses, CVA and CuVA. Considering the species demarcation criteria in the family and phylogenetic analyses, this virus should be considered as representing a new viral species in the genus Capillovirus, for which the name of Mume virus A is proposed. |
format | Online Article Text |
id | pubmed-5923438 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-59234382018-05-03 Molecular Characterization of a Novel Species of Capillovirus from Japanese Apricot (Prunus mume) Marais, Armelle Faure, Chantal Theil, Sébastien Candresse, Thierry Viruses Communication With the increased use of high-throughput sequencing methods, new viruses infecting Prunus spp. are being discovered and characterized, especially in the family Betaflexiviridae. Double-stranded RNAs from symptomatic leaves of a Japanese apricot (Prunus mume) tree from Japan were purified and analyzed by Illumina sequencing. Blast comparisons of reconstructed contigs showed that the P. mume sample was infected by a putative novel virus with homologies to Cherry virus A (CVA) and to the newly described Currant virus A (CuVA), both members of genus Capillovirus. Completion of the genome showed the new agent to have a genomic organization typical of capilloviruses, with two overlapping open reading frames encoding a large replication-associated protein fused to the coat protein (CP), and a putative movement protein (MP). This virus shares only, respectively, 63.2% and 62.7% CP amino acid identity with the most closely related viruses, CVA and CuVA. Considering the species demarcation criteria in the family and phylogenetic analyses, this virus should be considered as representing a new viral species in the genus Capillovirus, for which the name of Mume virus A is proposed. MDPI 2018-03-23 /pmc/articles/PMC5923438/ /pubmed/29570605 http://dx.doi.org/10.3390/v10040144 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Marais, Armelle Faure, Chantal Theil, Sébastien Candresse, Thierry Molecular Characterization of a Novel Species of Capillovirus from Japanese Apricot (Prunus mume) |
title | Molecular Characterization of a Novel Species of Capillovirus from Japanese Apricot (Prunus mume) |
title_full | Molecular Characterization of a Novel Species of Capillovirus from Japanese Apricot (Prunus mume) |
title_fullStr | Molecular Characterization of a Novel Species of Capillovirus from Japanese Apricot (Prunus mume) |
title_full_unstemmed | Molecular Characterization of a Novel Species of Capillovirus from Japanese Apricot (Prunus mume) |
title_short | Molecular Characterization of a Novel Species of Capillovirus from Japanese Apricot (Prunus mume) |
title_sort | molecular characterization of a novel species of capillovirus from japanese apricot (prunus mume) |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923438/ https://www.ncbi.nlm.nih.gov/pubmed/29570605 http://dx.doi.org/10.3390/v10040144 |
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