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Identification of Ellagic Acid from Plant Rhodiola rosea L. as an Anti-Ebola Virus Entry Inhibitor

The recent 2014–2016 West African Ebola virus epidemic underscores the need for the development of novel anti-Ebola therapeutics, due to the high mortality rates of Ebola virus infections and the lack of FDA-approved vaccine or therapy that is available for the prevention and treatment. Traditional...

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Autores principales: Cui, Qinghua, Du, Ruikun, Anantpadma, Manu, Schafer, Adam, Hou, Lin, Tian, Jingzhen, Davey, Robert A., Cheng, Han, Rong, Lijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923446/
https://www.ncbi.nlm.nih.gov/pubmed/29584652
http://dx.doi.org/10.3390/v10040152
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author Cui, Qinghua
Du, Ruikun
Anantpadma, Manu
Schafer, Adam
Hou, Lin
Tian, Jingzhen
Davey, Robert A.
Cheng, Han
Rong, Lijun
author_facet Cui, Qinghua
Du, Ruikun
Anantpadma, Manu
Schafer, Adam
Hou, Lin
Tian, Jingzhen
Davey, Robert A.
Cheng, Han
Rong, Lijun
author_sort Cui, Qinghua
collection PubMed
description The recent 2014–2016 West African Ebola virus epidemic underscores the need for the development of novel anti-Ebola therapeutics, due to the high mortality rates of Ebola virus infections and the lack of FDA-approved vaccine or therapy that is available for the prevention and treatment. Traditional Chinese medicines (TCMs) represent a huge reservoir of bioactive chemicals and many TCMs have been shown to have antiviral activities. 373 extracts from 128 TCMs were evaluated using a high throughput assay to screen for inhibitors of Ebola virus cell entry. Extract of Rhodiola rosea displayed specific and potent inhibition against cell entry of both Ebola virus and Marburg virus. In addition, twenty commercial compounds that were isolated from Rhodiola rosea were evaluated using the pseudotyped Ebola virus entry assay, and it was found that ellagic acid and gallic acid, which are two structurally related compounds, are the most effective ones. The activity of the extract and the two pure compounds were validated using infectious Ebola virus. The time-of-addition experiments suggest that, mechanistically, the Rhodiola rosea extract and the effective compounds act at an early step in the infection cycle following initial cell attachment, but prior to viral/cell membrane fusion. Our findings provide evidence that Rhodiola rosea has potent anti-filovirus properties that may be developed as a novel anti-Ebola treatment.
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spelling pubmed-59234462018-05-03 Identification of Ellagic Acid from Plant Rhodiola rosea L. as an Anti-Ebola Virus Entry Inhibitor Cui, Qinghua Du, Ruikun Anantpadma, Manu Schafer, Adam Hou, Lin Tian, Jingzhen Davey, Robert A. Cheng, Han Rong, Lijun Viruses Article The recent 2014–2016 West African Ebola virus epidemic underscores the need for the development of novel anti-Ebola therapeutics, due to the high mortality rates of Ebola virus infections and the lack of FDA-approved vaccine or therapy that is available for the prevention and treatment. Traditional Chinese medicines (TCMs) represent a huge reservoir of bioactive chemicals and many TCMs have been shown to have antiviral activities. 373 extracts from 128 TCMs were evaluated using a high throughput assay to screen for inhibitors of Ebola virus cell entry. Extract of Rhodiola rosea displayed specific and potent inhibition against cell entry of both Ebola virus and Marburg virus. In addition, twenty commercial compounds that were isolated from Rhodiola rosea were evaluated using the pseudotyped Ebola virus entry assay, and it was found that ellagic acid and gallic acid, which are two structurally related compounds, are the most effective ones. The activity of the extract and the two pure compounds were validated using infectious Ebola virus. The time-of-addition experiments suggest that, mechanistically, the Rhodiola rosea extract and the effective compounds act at an early step in the infection cycle following initial cell attachment, but prior to viral/cell membrane fusion. Our findings provide evidence that Rhodiola rosea has potent anti-filovirus properties that may be developed as a novel anti-Ebola treatment. MDPI 2018-03-27 /pmc/articles/PMC5923446/ /pubmed/29584652 http://dx.doi.org/10.3390/v10040152 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cui, Qinghua
Du, Ruikun
Anantpadma, Manu
Schafer, Adam
Hou, Lin
Tian, Jingzhen
Davey, Robert A.
Cheng, Han
Rong, Lijun
Identification of Ellagic Acid from Plant Rhodiola rosea L. as an Anti-Ebola Virus Entry Inhibitor
title Identification of Ellagic Acid from Plant Rhodiola rosea L. as an Anti-Ebola Virus Entry Inhibitor
title_full Identification of Ellagic Acid from Plant Rhodiola rosea L. as an Anti-Ebola Virus Entry Inhibitor
title_fullStr Identification of Ellagic Acid from Plant Rhodiola rosea L. as an Anti-Ebola Virus Entry Inhibitor
title_full_unstemmed Identification of Ellagic Acid from Plant Rhodiola rosea L. as an Anti-Ebola Virus Entry Inhibitor
title_short Identification of Ellagic Acid from Plant Rhodiola rosea L. as an Anti-Ebola Virus Entry Inhibitor
title_sort identification of ellagic acid from plant rhodiola rosea l. as an anti-ebola virus entry inhibitor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923446/
https://www.ncbi.nlm.nih.gov/pubmed/29584652
http://dx.doi.org/10.3390/v10040152
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