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Rapid CRISPR/Cas9-Mediated Cloning of Full-Length Epstein-Barr Virus Genomes from Latently Infected Cells

Herpesviruses have relatively large DNA genomes of more than 150 kb that are difficult to clone and sequence. Bacterial artificial chromosome (BAC) cloning of herpesvirus genomes is a powerful technique that greatly facilitates whole viral genome sequencing as well as functional characterization of...

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Detalles Bibliográficos
Autores principales:	Yajima, Misako, Ikuta, Kazufumi, Kanda, Teru
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2018
Materias:	Review
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923465/ https://www.ncbi.nlm.nih.gov/pubmed/29614006 http://dx.doi.org/10.3390/v10040171

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