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Urinary Cotinine Concentration and Self-Reported Smoking Status in 1075 Subjects Living in Central Italy

Background: Urinary cotinine, a metabolite of nicotine, is a marker of tobacco smoke exposure. A cutoff value for cotinine concentration can be set to distinguish smokers from non-smokers, independently from self-declared status. Method: Cotinine was determined by isotopic dilution High Performance...

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Autores principales: Paci, Enrico, Pigini, Daniela, Bauleo, Lisa, Ancona, Carla, Forastiere, Francesco, Tranfo, Giovanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923846/
https://www.ncbi.nlm.nih.gov/pubmed/29671826
http://dx.doi.org/10.3390/ijerph15040804
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author Paci, Enrico
Pigini, Daniela
Bauleo, Lisa
Ancona, Carla
Forastiere, Francesco
Tranfo, Giovanna
author_facet Paci, Enrico
Pigini, Daniela
Bauleo, Lisa
Ancona, Carla
Forastiere, Francesco
Tranfo, Giovanna
author_sort Paci, Enrico
collection PubMed
description Background: Urinary cotinine, a metabolite of nicotine, is a marker of tobacco smoke exposure. A cutoff value for cotinine concentration can be set to distinguish smokers from non-smokers, independently from self-declared status. Method: Cotinine was determined by isotopic dilution High Performance Liquid Chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) between 2013 and 2014 on urine samples of a population of 1075 subjects. Results: 296 subjects have a cotinine level higher than 100 μg/g of creatinine (cutoff), with a median cotinine concentration of 1504.70 μg/g of creatinine. The mean is 27.5% of smokers and 60.5% in this group are females. The median value for non-smokers is 5.6 μg/g of creatinine. Two hundred and seventy-five subjects declared to be smokers in the questionnaire, but 6 (2.2%) present urinary cotinine levels lower than cutoff; 800 subjects declared to be non-smokers, but 26 of them presented urinary cotinine levels that were higher than the cutoff (3.3%). Conclusion: Using the cutoff of 100 μg/g, the misclassification of smokers resulted to be 2.2%, indicating that the selected value is suitable for studying the human exposures to environmental and occupational pollutants, including those produced by smoking.
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spelling pubmed-59238462018-05-03 Urinary Cotinine Concentration and Self-Reported Smoking Status in 1075 Subjects Living in Central Italy Paci, Enrico Pigini, Daniela Bauleo, Lisa Ancona, Carla Forastiere, Francesco Tranfo, Giovanna Int J Environ Res Public Health Article Background: Urinary cotinine, a metabolite of nicotine, is a marker of tobacco smoke exposure. A cutoff value for cotinine concentration can be set to distinguish smokers from non-smokers, independently from self-declared status. Method: Cotinine was determined by isotopic dilution High Performance Liquid Chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) between 2013 and 2014 on urine samples of a population of 1075 subjects. Results: 296 subjects have a cotinine level higher than 100 μg/g of creatinine (cutoff), with a median cotinine concentration of 1504.70 μg/g of creatinine. The mean is 27.5% of smokers and 60.5% in this group are females. The median value for non-smokers is 5.6 μg/g of creatinine. Two hundred and seventy-five subjects declared to be smokers in the questionnaire, but 6 (2.2%) present urinary cotinine levels lower than cutoff; 800 subjects declared to be non-smokers, but 26 of them presented urinary cotinine levels that were higher than the cutoff (3.3%). Conclusion: Using the cutoff of 100 μg/g, the misclassification of smokers resulted to be 2.2%, indicating that the selected value is suitable for studying the human exposures to environmental and occupational pollutants, including those produced by smoking. MDPI 2018-04-19 2018-04 /pmc/articles/PMC5923846/ /pubmed/29671826 http://dx.doi.org/10.3390/ijerph15040804 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Paci, Enrico
Pigini, Daniela
Bauleo, Lisa
Ancona, Carla
Forastiere, Francesco
Tranfo, Giovanna
Urinary Cotinine Concentration and Self-Reported Smoking Status in 1075 Subjects Living in Central Italy
title Urinary Cotinine Concentration and Self-Reported Smoking Status in 1075 Subjects Living in Central Italy
title_full Urinary Cotinine Concentration and Self-Reported Smoking Status in 1075 Subjects Living in Central Italy
title_fullStr Urinary Cotinine Concentration and Self-Reported Smoking Status in 1075 Subjects Living in Central Italy
title_full_unstemmed Urinary Cotinine Concentration and Self-Reported Smoking Status in 1075 Subjects Living in Central Italy
title_short Urinary Cotinine Concentration and Self-Reported Smoking Status in 1075 Subjects Living in Central Italy
title_sort urinary cotinine concentration and self-reported smoking status in 1075 subjects living in central italy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923846/
https://www.ncbi.nlm.nih.gov/pubmed/29671826
http://dx.doi.org/10.3390/ijerph15040804
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