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Knockdown of the lncRNA SNHG8 inhibits cell growth in Epstein-Barr virus-associated gastric carcinoma

BACKGROUND: Epstein–Barr virus (EBV) infection is causatively associated with a variety of human cancers, including gastric cancer (GC), which has one of the highest mortality rates of all human cancers. Long non-coding RNAs (lncRNAs) show important regulatory roles in human GC. SNHG8 is a recently...

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Autores principales: Liu, Jing, Yang, Chunxia, Gu, Yufang, Li, Chong, Zhang, Huamei, Zhang, Wenfang, Wang, Xueqing, Wu, Nan, Zheng, Chunyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5924468/
https://www.ncbi.nlm.nih.gov/pubmed/29736176
http://dx.doi.org/10.1186/s11658-018-0070-8
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author Liu, Jing
Yang, Chunxia
Gu, Yufang
Li, Chong
Zhang, Huamei
Zhang, Wenfang
Wang, Xueqing
Wu, Nan
Zheng, Chunyan
author_facet Liu, Jing
Yang, Chunxia
Gu, Yufang
Li, Chong
Zhang, Huamei
Zhang, Wenfang
Wang, Xueqing
Wu, Nan
Zheng, Chunyan
author_sort Liu, Jing
collection PubMed
description BACKGROUND: Epstein–Barr virus (EBV) infection is causatively associated with a variety of human cancers, including gastric cancer (GC), which has one of the highest mortality rates of all human cancers. Long non-coding RNAs (lncRNAs) show important regulatory roles in human GC. SNHG8 is a recently identified lncRNA that was reported to show abnormal expression pattern in GC. However, little is known of its biological function in EBV-associated GC. METHODS: We used cell viability, colony formation and cell cycle assays to investigate the roles of lncRNA SNHG8 in the cell growth of EBV-associated GC. RESULTS: The transcript levels of SNHG8 in the cultured EBV-associated GC cells were significantly higher in the cultured EBV-associated GC cells compared with the levels in normal human gastric mucosal cells and EBV-negative GC cells. Knockdown of SNHG8 with specific shRNAs inhibited cell proliferation and colony formation and arrested the cell cycle in the G0/G1 phase in vitro. We also found that knockdown of SNHG8 suppressed tumor growth in vivo. CONCLUSIONS: These data indicate the pro-oncogenic potential of SNHG8 in EBV-associated GC, meaning it is a latent therapeutic target for the treatment of this type of cancer.
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spelling pubmed-59244682018-05-07 Knockdown of the lncRNA SNHG8 inhibits cell growth in Epstein-Barr virus-associated gastric carcinoma Liu, Jing Yang, Chunxia Gu, Yufang Li, Chong Zhang, Huamei Zhang, Wenfang Wang, Xueqing Wu, Nan Zheng, Chunyan Cell Mol Biol Lett Research BACKGROUND: Epstein–Barr virus (EBV) infection is causatively associated with a variety of human cancers, including gastric cancer (GC), which has one of the highest mortality rates of all human cancers. Long non-coding RNAs (lncRNAs) show important regulatory roles in human GC. SNHG8 is a recently identified lncRNA that was reported to show abnormal expression pattern in GC. However, little is known of its biological function in EBV-associated GC. METHODS: We used cell viability, colony formation and cell cycle assays to investigate the roles of lncRNA SNHG8 in the cell growth of EBV-associated GC. RESULTS: The transcript levels of SNHG8 in the cultured EBV-associated GC cells were significantly higher in the cultured EBV-associated GC cells compared with the levels in normal human gastric mucosal cells and EBV-negative GC cells. Knockdown of SNHG8 with specific shRNAs inhibited cell proliferation and colony formation and arrested the cell cycle in the G0/G1 phase in vitro. We also found that knockdown of SNHG8 suppressed tumor growth in vivo. CONCLUSIONS: These data indicate the pro-oncogenic potential of SNHG8 in EBV-associated GC, meaning it is a latent therapeutic target for the treatment of this type of cancer. BioMed Central 2018-04-27 /pmc/articles/PMC5924468/ /pubmed/29736176 http://dx.doi.org/10.1186/s11658-018-0070-8 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Liu, Jing
Yang, Chunxia
Gu, Yufang
Li, Chong
Zhang, Huamei
Zhang, Wenfang
Wang, Xueqing
Wu, Nan
Zheng, Chunyan
Knockdown of the lncRNA SNHG8 inhibits cell growth in Epstein-Barr virus-associated gastric carcinoma
title Knockdown of the lncRNA SNHG8 inhibits cell growth in Epstein-Barr virus-associated gastric carcinoma
title_full Knockdown of the lncRNA SNHG8 inhibits cell growth in Epstein-Barr virus-associated gastric carcinoma
title_fullStr Knockdown of the lncRNA SNHG8 inhibits cell growth in Epstein-Barr virus-associated gastric carcinoma
title_full_unstemmed Knockdown of the lncRNA SNHG8 inhibits cell growth in Epstein-Barr virus-associated gastric carcinoma
title_short Knockdown of the lncRNA SNHG8 inhibits cell growth in Epstein-Barr virus-associated gastric carcinoma
title_sort knockdown of the lncrna snhg8 inhibits cell growth in epstein-barr virus-associated gastric carcinoma
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5924468/
https://www.ncbi.nlm.nih.gov/pubmed/29736176
http://dx.doi.org/10.1186/s11658-018-0070-8
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