Cargando…
A Possible Trifunctional β-Carotene Synthase Gene Identified in the Draft Genome of Aurantiochytrium sp. Strain KH105
Labyrinthulomycetes have been regarded as a promising industrial source of xanthophylls, including astaxanthin and canthaxanthin, polyunsaturated fatty acids such as docosahexaenoic acid and docosapentaenoic acid, ω-3 oils, and terpenic hydrocarbons, such as sterols and squalene. A Thraustochytrid,...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5924542/ https://www.ncbi.nlm.nih.gov/pubmed/29642531 http://dx.doi.org/10.3390/genes9040200 |
_version_ | 1783318571293605888 |
---|---|
author | Iwasaka, Hiroaki Koyanagi, Ryo Satoh, Ryota Nagano, Akiko Watanabe, Kenshi Hisata, Kanako Satoh, Noriyuki Aki, Tsunehiro |
author_facet | Iwasaka, Hiroaki Koyanagi, Ryo Satoh, Ryota Nagano, Akiko Watanabe, Kenshi Hisata, Kanako Satoh, Noriyuki Aki, Tsunehiro |
author_sort | Iwasaka, Hiroaki |
collection | PubMed |
description | Labyrinthulomycetes have been regarded as a promising industrial source of xanthophylls, including astaxanthin and canthaxanthin, polyunsaturated fatty acids such as docosahexaenoic acid and docosapentaenoic acid, ω-3 oils, and terpenic hydrocarbons, such as sterols and squalene. A Thraustochytrid, Aurantiochytrium sp. KH105 produces carotenoids, including astaxanthin, with strong antioxidant activity. To gain genomic insights into this capacity, we decoded its 97-Mbp genome and characterized genes for enzymes involved in carotenoid biosynthesis. Interestingly, all carotenogenic genes, as well as other eukaryotic genes, appeared duplicated, suggesting that this strain is diploid. In addition, among the five genes involved in the pathway from geranylgeranyl pyrophosphate to astaxanthin, geranylgeranyl phytoene synthase (crtB), phytoene desaturase (crtI) and lycopene cyclase (crtY) were fused into single gene (crtIBY) with no internal stop codons. Functionality of the trifunctional enzyme, CrtIBY, to catalyze the reaction from geranylgeranyl diphosphate to β-carotene was confirmed using a yeast assay system and mass spectrometry. Furthermore, analyses of differential gene expression showed characteristic up-regulation of carotenoid biosynthetic genes during stationary and starvation phases under these culture conditions. This suggests genetic engineering events to promote more efficient production of carotenoids. We also showed an occurrence of crtIBY in other Thraustochytrid species. |
format | Online Article Text |
id | pubmed-5924542 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-59245422018-05-03 A Possible Trifunctional β-Carotene Synthase Gene Identified in the Draft Genome of Aurantiochytrium sp. Strain KH105 Iwasaka, Hiroaki Koyanagi, Ryo Satoh, Ryota Nagano, Akiko Watanabe, Kenshi Hisata, Kanako Satoh, Noriyuki Aki, Tsunehiro Genes (Basel) Article Labyrinthulomycetes have been regarded as a promising industrial source of xanthophylls, including astaxanthin and canthaxanthin, polyunsaturated fatty acids such as docosahexaenoic acid and docosapentaenoic acid, ω-3 oils, and terpenic hydrocarbons, such as sterols and squalene. A Thraustochytrid, Aurantiochytrium sp. KH105 produces carotenoids, including astaxanthin, with strong antioxidant activity. To gain genomic insights into this capacity, we decoded its 97-Mbp genome and characterized genes for enzymes involved in carotenoid biosynthesis. Interestingly, all carotenogenic genes, as well as other eukaryotic genes, appeared duplicated, suggesting that this strain is diploid. In addition, among the five genes involved in the pathway from geranylgeranyl pyrophosphate to astaxanthin, geranylgeranyl phytoene synthase (crtB), phytoene desaturase (crtI) and lycopene cyclase (crtY) were fused into single gene (crtIBY) with no internal stop codons. Functionality of the trifunctional enzyme, CrtIBY, to catalyze the reaction from geranylgeranyl diphosphate to β-carotene was confirmed using a yeast assay system and mass spectrometry. Furthermore, analyses of differential gene expression showed characteristic up-regulation of carotenoid biosynthetic genes during stationary and starvation phases under these culture conditions. This suggests genetic engineering events to promote more efficient production of carotenoids. We also showed an occurrence of crtIBY in other Thraustochytrid species. MDPI 2018-04-09 /pmc/articles/PMC5924542/ /pubmed/29642531 http://dx.doi.org/10.3390/genes9040200 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Iwasaka, Hiroaki Koyanagi, Ryo Satoh, Ryota Nagano, Akiko Watanabe, Kenshi Hisata, Kanako Satoh, Noriyuki Aki, Tsunehiro A Possible Trifunctional β-Carotene Synthase Gene Identified in the Draft Genome of Aurantiochytrium sp. Strain KH105 |
title | A Possible Trifunctional β-Carotene Synthase Gene Identified in the Draft Genome of Aurantiochytrium sp. Strain KH105 |
title_full | A Possible Trifunctional β-Carotene Synthase Gene Identified in the Draft Genome of Aurantiochytrium sp. Strain KH105 |
title_fullStr | A Possible Trifunctional β-Carotene Synthase Gene Identified in the Draft Genome of Aurantiochytrium sp. Strain KH105 |
title_full_unstemmed | A Possible Trifunctional β-Carotene Synthase Gene Identified in the Draft Genome of Aurantiochytrium sp. Strain KH105 |
title_short | A Possible Trifunctional β-Carotene Synthase Gene Identified in the Draft Genome of Aurantiochytrium sp. Strain KH105 |
title_sort | possible trifunctional β-carotene synthase gene identified in the draft genome of aurantiochytrium sp. strain kh105 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5924542/ https://www.ncbi.nlm.nih.gov/pubmed/29642531 http://dx.doi.org/10.3390/genes9040200 |
work_keys_str_mv | AT iwasakahiroaki apossibletrifunctionalbcarotenesynthasegeneidentifiedinthedraftgenomeofaurantiochytriumspstrainkh105 AT koyanagiryo apossibletrifunctionalbcarotenesynthasegeneidentifiedinthedraftgenomeofaurantiochytriumspstrainkh105 AT satohryota apossibletrifunctionalbcarotenesynthasegeneidentifiedinthedraftgenomeofaurantiochytriumspstrainkh105 AT naganoakiko apossibletrifunctionalbcarotenesynthasegeneidentifiedinthedraftgenomeofaurantiochytriumspstrainkh105 AT watanabekenshi apossibletrifunctionalbcarotenesynthasegeneidentifiedinthedraftgenomeofaurantiochytriumspstrainkh105 AT hisatakanako apossibletrifunctionalbcarotenesynthasegeneidentifiedinthedraftgenomeofaurantiochytriumspstrainkh105 AT satohnoriyuki apossibletrifunctionalbcarotenesynthasegeneidentifiedinthedraftgenomeofaurantiochytriumspstrainkh105 AT akitsunehiro apossibletrifunctionalbcarotenesynthasegeneidentifiedinthedraftgenomeofaurantiochytriumspstrainkh105 AT iwasakahiroaki possibletrifunctionalbcarotenesynthasegeneidentifiedinthedraftgenomeofaurantiochytriumspstrainkh105 AT koyanagiryo possibletrifunctionalbcarotenesynthasegeneidentifiedinthedraftgenomeofaurantiochytriumspstrainkh105 AT satohryota possibletrifunctionalbcarotenesynthasegeneidentifiedinthedraftgenomeofaurantiochytriumspstrainkh105 AT naganoakiko possibletrifunctionalbcarotenesynthasegeneidentifiedinthedraftgenomeofaurantiochytriumspstrainkh105 AT watanabekenshi possibletrifunctionalbcarotenesynthasegeneidentifiedinthedraftgenomeofaurantiochytriumspstrainkh105 AT hisatakanako possibletrifunctionalbcarotenesynthasegeneidentifiedinthedraftgenomeofaurantiochytriumspstrainkh105 AT satohnoriyuki possibletrifunctionalbcarotenesynthasegeneidentifiedinthedraftgenomeofaurantiochytriumspstrainkh105 AT akitsunehiro possibletrifunctionalbcarotenesynthasegeneidentifiedinthedraftgenomeofaurantiochytriumspstrainkh105 |