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Toxoplasma gondii in Blood Donors: A Study in Boyer-Ahmad County, Southwest Iran

Toxoplasma gondii is an important foodborne protozoan that can be transmitted through infected blood containing tachyzoite form of the parasite. The current study aimed to evaluate the prevalence of T. gondii infection and related risk factors among healthy blood donors in Boyer-Ahmad County, southw...

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Autores principales: Moshfe, Abdolali, Arefkhah, Nasir, Sarkari, Bahador, Kazemi, Saadat, Mardani, Ahmad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5925030/
https://www.ncbi.nlm.nih.gov/pubmed/29849610
http://dx.doi.org/10.1155/2018/3813612
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author Moshfe, Abdolali
Arefkhah, Nasir
Sarkari, Bahador
Kazemi, Saadat
Mardani, Ahmad
author_facet Moshfe, Abdolali
Arefkhah, Nasir
Sarkari, Bahador
Kazemi, Saadat
Mardani, Ahmad
author_sort Moshfe, Abdolali
collection PubMed
description Toxoplasma gondii is an important foodborne protozoan that can be transmitted through infected blood containing tachyzoite form of the parasite. The current study aimed to evaluate the prevalence of T. gondii infection and related risk factors among healthy blood donors in Boyer-Ahmad County, southwest Iran. Blood samples were taken from 285 healthy blood donors who voluntarily agreed to participate in this study. Sera and buffy coat were isolated from the blood samples for serological and molecular evaluations. The sera were tested for anti-T. gondii antibodies (both IgG and IgM), using a commercial ELISA kit. The buffy coat of seropositive cases was evaluated for detection of T. gondii DNA by PCR. Moreover, a structured questionnaire, containing socioepidemiological data and possible risk factors, was filled out by each participant during sample collection. Anti-T. gondii antibodies were detected in sera of 48/285 (16.8%) participants. Only two of the subjects (0.7%) were seropositive for both IgG and IgM antibodies. T. gondii DNA was not detected in buffy coat of any of the seropositive cases. Risk factors such as contact with soil (OR, 9.7; 95% CI, 4.9–19.4) and consumption of semicooked meat (OR, 2.5; 95% CI, 1.2–5.03) were statistically associated with seropositivity to T. gondii. The seroprevalence rate of T. gondii antibodies in the blood donors of Boyer-Ahmad County was not high in comparison with other regions in Iran. In this study, consumption of undercooked meats, job, and contact with soil were independent risk factors associated with T. gondii infection, which can be considered as potential sources of T. gondii infection.
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spelling pubmed-59250302018-05-30 Toxoplasma gondii in Blood Donors: A Study in Boyer-Ahmad County, Southwest Iran Moshfe, Abdolali Arefkhah, Nasir Sarkari, Bahador Kazemi, Saadat Mardani, Ahmad Interdiscip Perspect Infect Dis Research Article Toxoplasma gondii is an important foodborne protozoan that can be transmitted through infected blood containing tachyzoite form of the parasite. The current study aimed to evaluate the prevalence of T. gondii infection and related risk factors among healthy blood donors in Boyer-Ahmad County, southwest Iran. Blood samples were taken from 285 healthy blood donors who voluntarily agreed to participate in this study. Sera and buffy coat were isolated from the blood samples for serological and molecular evaluations. The sera were tested for anti-T. gondii antibodies (both IgG and IgM), using a commercial ELISA kit. The buffy coat of seropositive cases was evaluated for detection of T. gondii DNA by PCR. Moreover, a structured questionnaire, containing socioepidemiological data and possible risk factors, was filled out by each participant during sample collection. Anti-T. gondii antibodies were detected in sera of 48/285 (16.8%) participants. Only two of the subjects (0.7%) were seropositive for both IgG and IgM antibodies. T. gondii DNA was not detected in buffy coat of any of the seropositive cases. Risk factors such as contact with soil (OR, 9.7; 95% CI, 4.9–19.4) and consumption of semicooked meat (OR, 2.5; 95% CI, 1.2–5.03) were statistically associated with seropositivity to T. gondii. The seroprevalence rate of T. gondii antibodies in the blood donors of Boyer-Ahmad County was not high in comparison with other regions in Iran. In this study, consumption of undercooked meats, job, and contact with soil were independent risk factors associated with T. gondii infection, which can be considered as potential sources of T. gondii infection. Hindawi 2018-04-15 /pmc/articles/PMC5925030/ /pubmed/29849610 http://dx.doi.org/10.1155/2018/3813612 Text en Copyright © 2018 Abdolali Moshfe et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Moshfe, Abdolali
Arefkhah, Nasir
Sarkari, Bahador
Kazemi, Saadat
Mardani, Ahmad
Toxoplasma gondii in Blood Donors: A Study in Boyer-Ahmad County, Southwest Iran
title Toxoplasma gondii in Blood Donors: A Study in Boyer-Ahmad County, Southwest Iran
title_full Toxoplasma gondii in Blood Donors: A Study in Boyer-Ahmad County, Southwest Iran
title_fullStr Toxoplasma gondii in Blood Donors: A Study in Boyer-Ahmad County, Southwest Iran
title_full_unstemmed Toxoplasma gondii in Blood Donors: A Study in Boyer-Ahmad County, Southwest Iran
title_short Toxoplasma gondii in Blood Donors: A Study in Boyer-Ahmad County, Southwest Iran
title_sort toxoplasma gondii in blood donors: a study in boyer-ahmad county, southwest iran
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5925030/
https://www.ncbi.nlm.nih.gov/pubmed/29849610
http://dx.doi.org/10.1155/2018/3813612
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