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Genomic survey of bZIP transcription factor genes related to tanshinone biosynthesis in Salvia miltiorrhiza

Tanshinones are a class of bioactive components in the traditional Chinese medicine Salvia miltiorrhiza, and their biosynthesis and regulation have been widely studied. Current studies show that basic leucine zipper (bZIP) proteins regulate plant secondary metabolism, growth and developmental proces...

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Autores principales: Zhang, Yu, Xu, Zhichao, Ji, Aijia, Luo, Hongmei, Song, Jingyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5925414/
https://www.ncbi.nlm.nih.gov/pubmed/29719790
http://dx.doi.org/10.1016/j.apsb.2017.09.002
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author Zhang, Yu
Xu, Zhichao
Ji, Aijia
Luo, Hongmei
Song, Jingyuan
author_facet Zhang, Yu
Xu, Zhichao
Ji, Aijia
Luo, Hongmei
Song, Jingyuan
author_sort Zhang, Yu
collection PubMed
description Tanshinones are a class of bioactive components in the traditional Chinese medicine Salvia miltiorrhiza, and their biosynthesis and regulation have been widely studied. Current studies show that basic leucine zipper (bZIP) proteins regulate plant secondary metabolism, growth and developmental processes. However, the bZIP transcription factors involved in tanshinone biosynthesis are unknown. Here, we conducted the first genome-wide survey of the bZIP gene family and analyzed the phylogeny, gene structure, additional conserved motifs and alternative splicing events in S. miltiorrhiza. A total of 70 SmbZIP transcription factors were identified and categorized into 11 subgroups based on their phylogenetic relationships with those in Arabidopsis. Moreover, seventeen SmbZIP genes underwent alternative splicing events. According to the transcriptomic data, the SmbZIP genes that were highly expressed in the Danshen root and periderm were selected. Based on the prediction of bZIP binding sites in the promoters and the co-expression analysis and co-induction patterns in response to Ag(+) treatment via quantitative real-time polymerase chain reaction (qRT-PCR), we concluded that SmbZIP7 and SmbZIP20 potentially participate in the regulation of tanshinone biosynthesis. These results provide a foundation for further functional characterization of the candidate SmbZIP genes, which have the potential to increase tanshinone production.
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spelling pubmed-59254142018-05-01 Genomic survey of bZIP transcription factor genes related to tanshinone biosynthesis in Salvia miltiorrhiza Zhang, Yu Xu, Zhichao Ji, Aijia Luo, Hongmei Song, Jingyuan Acta Pharm Sin B Original Article Tanshinones are a class of bioactive components in the traditional Chinese medicine Salvia miltiorrhiza, and their biosynthesis and regulation have been widely studied. Current studies show that basic leucine zipper (bZIP) proteins regulate plant secondary metabolism, growth and developmental processes. However, the bZIP transcription factors involved in tanshinone biosynthesis are unknown. Here, we conducted the first genome-wide survey of the bZIP gene family and analyzed the phylogeny, gene structure, additional conserved motifs and alternative splicing events in S. miltiorrhiza. A total of 70 SmbZIP transcription factors were identified and categorized into 11 subgroups based on their phylogenetic relationships with those in Arabidopsis. Moreover, seventeen SmbZIP genes underwent alternative splicing events. According to the transcriptomic data, the SmbZIP genes that were highly expressed in the Danshen root and periderm were selected. Based on the prediction of bZIP binding sites in the promoters and the co-expression analysis and co-induction patterns in response to Ag(+) treatment via quantitative real-time polymerase chain reaction (qRT-PCR), we concluded that SmbZIP7 and SmbZIP20 potentially participate in the regulation of tanshinone biosynthesis. These results provide a foundation for further functional characterization of the candidate SmbZIP genes, which have the potential to increase tanshinone production. Elsevier 2018-03 2017-10-05 /pmc/articles/PMC5925414/ /pubmed/29719790 http://dx.doi.org/10.1016/j.apsb.2017.09.002 Text en © 2018 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Zhang, Yu
Xu, Zhichao
Ji, Aijia
Luo, Hongmei
Song, Jingyuan
Genomic survey of bZIP transcription factor genes related to tanshinone biosynthesis in Salvia miltiorrhiza
title Genomic survey of bZIP transcription factor genes related to tanshinone biosynthesis in Salvia miltiorrhiza
title_full Genomic survey of bZIP transcription factor genes related to tanshinone biosynthesis in Salvia miltiorrhiza
title_fullStr Genomic survey of bZIP transcription factor genes related to tanshinone biosynthesis in Salvia miltiorrhiza
title_full_unstemmed Genomic survey of bZIP transcription factor genes related to tanshinone biosynthesis in Salvia miltiorrhiza
title_short Genomic survey of bZIP transcription factor genes related to tanshinone biosynthesis in Salvia miltiorrhiza
title_sort genomic survey of bzip transcription factor genes related to tanshinone biosynthesis in salvia miltiorrhiza
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5925414/
https://www.ncbi.nlm.nih.gov/pubmed/29719790
http://dx.doi.org/10.1016/j.apsb.2017.09.002
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