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Comparative analysis of human sperm glycocalyx from different freezability ejaculates by lectin microarray and identification of ABA as sperm freezability biomarker
BACKGROUND: Semen cryopreservation has been widely applied in assisted reproductive technologies and sperm bank, but it causes considerable impairments on sperm quality. It is necessary to find an evaluation indicator for determining the sperm-freezing tolerance. METHODS: The glycocalyx of good free...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5925848/ https://www.ncbi.nlm.nih.gov/pubmed/29719495 http://dx.doi.org/10.1186/s12014-018-9195-z |
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author | Xin, Ai-jie Wu, Yan-cheng Lu, Hui Cheng, Li Gu, Yi-hua Diao, Hua Chen, Guo-wu Wu, Bin Li, Zheng Tao, Sheng-ce Sun, Xiao-xi Shi, Hui-juan |
author_facet | Xin, Ai-jie Wu, Yan-cheng Lu, Hui Cheng, Li Gu, Yi-hua Diao, Hua Chen, Guo-wu Wu, Bin Li, Zheng Tao, Sheng-ce Sun, Xiao-xi Shi, Hui-juan |
author_sort | Xin, Ai-jie |
collection | PubMed |
description | BACKGROUND: Semen cryopreservation has been widely applied in assisted reproductive technologies and sperm bank, but it causes considerable impairments on sperm quality. It is necessary to find an evaluation indicator for determining the sperm-freezing tolerance. METHODS: The glycocalyx of good freezability ejaculates was compared with poor freezability ejaculates by lectin microarray. The significant different lectins were validated by flow cytometry (FACS). To analyze the relationship between the potential biomarker and the tolerance of sperm to cryopreservation, 60 samples with different recovery rates were collected and detected the lectin-binding intensity by FACS. The receiver operating characteristic (ROC) curve was analyzed to test the capability of the lectin as a potential biomarker for detecting the sperm freezablility. RESULTS: ABA and DSL were found to develop significant differences between them. Further validation showed that ABA was significantly negative correlated with the sperm recovery rates (r = − 0.618, P < 0.000) and could be a potential biomarker for predicting sperm freezability (AUC = 0.733 ± 0.067, 95% CI 0.601 − 0.865, P < 0.01). CONCLUSION: ABA could be a potential biomarker for predicting sperm freezability. It will help to reduce sperm-freezing recovery tests and improve the efficiency of cryopreservation in human sperm bank. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12014-018-9195-z) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5925848 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-59258482018-05-01 Comparative analysis of human sperm glycocalyx from different freezability ejaculates by lectin microarray and identification of ABA as sperm freezability biomarker Xin, Ai-jie Wu, Yan-cheng Lu, Hui Cheng, Li Gu, Yi-hua Diao, Hua Chen, Guo-wu Wu, Bin Li, Zheng Tao, Sheng-ce Sun, Xiao-xi Shi, Hui-juan Clin Proteomics Research BACKGROUND: Semen cryopreservation has been widely applied in assisted reproductive technologies and sperm bank, but it causes considerable impairments on sperm quality. It is necessary to find an evaluation indicator for determining the sperm-freezing tolerance. METHODS: The glycocalyx of good freezability ejaculates was compared with poor freezability ejaculates by lectin microarray. The significant different lectins were validated by flow cytometry (FACS). To analyze the relationship between the potential biomarker and the tolerance of sperm to cryopreservation, 60 samples with different recovery rates were collected and detected the lectin-binding intensity by FACS. The receiver operating characteristic (ROC) curve was analyzed to test the capability of the lectin as a potential biomarker for detecting the sperm freezablility. RESULTS: ABA and DSL were found to develop significant differences between them. Further validation showed that ABA was significantly negative correlated with the sperm recovery rates (r = − 0.618, P < 0.000) and could be a potential biomarker for predicting sperm freezability (AUC = 0.733 ± 0.067, 95% CI 0.601 − 0.865, P < 0.01). CONCLUSION: ABA could be a potential biomarker for predicting sperm freezability. It will help to reduce sperm-freezing recovery tests and improve the efficiency of cryopreservation in human sperm bank. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12014-018-9195-z) contains supplementary material, which is available to authorized users. BioMed Central 2018-04-30 /pmc/articles/PMC5925848/ /pubmed/29719495 http://dx.doi.org/10.1186/s12014-018-9195-z Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Xin, Ai-jie Wu, Yan-cheng Lu, Hui Cheng, Li Gu, Yi-hua Diao, Hua Chen, Guo-wu Wu, Bin Li, Zheng Tao, Sheng-ce Sun, Xiao-xi Shi, Hui-juan Comparative analysis of human sperm glycocalyx from different freezability ejaculates by lectin microarray and identification of ABA as sperm freezability biomarker |
title | Comparative analysis of human sperm glycocalyx from different freezability ejaculates by lectin microarray and identification of ABA as sperm freezability biomarker |
title_full | Comparative analysis of human sperm glycocalyx from different freezability ejaculates by lectin microarray and identification of ABA as sperm freezability biomarker |
title_fullStr | Comparative analysis of human sperm glycocalyx from different freezability ejaculates by lectin microarray and identification of ABA as sperm freezability biomarker |
title_full_unstemmed | Comparative analysis of human sperm glycocalyx from different freezability ejaculates by lectin microarray and identification of ABA as sperm freezability biomarker |
title_short | Comparative analysis of human sperm glycocalyx from different freezability ejaculates by lectin microarray and identification of ABA as sperm freezability biomarker |
title_sort | comparative analysis of human sperm glycocalyx from different freezability ejaculates by lectin microarray and identification of aba as sperm freezability biomarker |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5925848/ https://www.ncbi.nlm.nih.gov/pubmed/29719495 http://dx.doi.org/10.1186/s12014-018-9195-z |
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