Cargando…
Lychee Seed Fraction Inhibits Aβ(1-42)-Induced Neuroinflammation in BV-2 Cells via NF-κB Signaling Pathway
In our previous studies, an active fraction derived from lychee seed could inhibit β-amyloid-induced apoptosis of PC12 cells and neurons. The primarily microglia cells are recognized as the brain’s resident macrophages and thought to remodel of the brain by removing presumably redundant, apoptotic n...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5925968/ https://www.ncbi.nlm.nih.gov/pubmed/29740316 http://dx.doi.org/10.3389/fphar.2018.00380 |
_version_ | 1783318806641246208 |
---|---|
author | Zhao, Ya Zeng, Yuan Wu, Anguo Yu, Chonglin Tang, Yong Wang, Xiuling Xiong, Rui Chen, Haixia Wu, Jianming Qin, Dalian |
author_facet | Zhao, Ya Zeng, Yuan Wu, Anguo Yu, Chonglin Tang, Yong Wang, Xiuling Xiong, Rui Chen, Haixia Wu, Jianming Qin, Dalian |
author_sort | Zhao, Ya |
collection | PubMed |
description | In our previous studies, an active fraction derived from lychee seed could inhibit β-amyloid-induced apoptosis of PC12 cells and neurons. The primarily microglia cells are recognized as the brain’s resident macrophages and thought to remodel of the brain by removing presumably redundant, apoptotic neurons. In the current study, we aimed to investigate the anti-neuroinflammation effect of lychee seed fraction (LSF) in Aβ(1-42)-induced BV-2 cells and the underlying mechanism. The morphology results displayed that LSF could improve the status of Aβ(1-42)-induced BV-2 cells. The enzyme-linked immunosorbent assay, real-time PCR, and Western blotting results showed that LSF could significantly reduce the release, mRNA levels, and protein expressions of the pro-inflammatory cytokines such as interleukin-1β (IL-1β), tumor necrosis factor alpha (TNF-α), cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) in Aβ(1-42)-induced BV-2 cells, which were downregulated through suppressing the NF-κB signaling pathway. Furthermore, LSF could upregulate Bcl-2 and downregulate Bax, Caspase-3, and cleaved-PARP protein expressions. Taken together, our results first demonstrated that LSF could suppress the inflammatory response via inhibiting NF-κB signaling pathway, and inhibit apoptosis in Aβ(1-42)-induced BV-2 cells. Our findings further prove that LSF as a potential drug may be used for treating AD in the future. |
format | Online Article Text |
id | pubmed-5925968 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-59259682018-05-08 Lychee Seed Fraction Inhibits Aβ(1-42)-Induced Neuroinflammation in BV-2 Cells via NF-κB Signaling Pathway Zhao, Ya Zeng, Yuan Wu, Anguo Yu, Chonglin Tang, Yong Wang, Xiuling Xiong, Rui Chen, Haixia Wu, Jianming Qin, Dalian Front Pharmacol Pharmacology In our previous studies, an active fraction derived from lychee seed could inhibit β-amyloid-induced apoptosis of PC12 cells and neurons. The primarily microglia cells are recognized as the brain’s resident macrophages and thought to remodel of the brain by removing presumably redundant, apoptotic neurons. In the current study, we aimed to investigate the anti-neuroinflammation effect of lychee seed fraction (LSF) in Aβ(1-42)-induced BV-2 cells and the underlying mechanism. The morphology results displayed that LSF could improve the status of Aβ(1-42)-induced BV-2 cells. The enzyme-linked immunosorbent assay, real-time PCR, and Western blotting results showed that LSF could significantly reduce the release, mRNA levels, and protein expressions of the pro-inflammatory cytokines such as interleukin-1β (IL-1β), tumor necrosis factor alpha (TNF-α), cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) in Aβ(1-42)-induced BV-2 cells, which were downregulated through suppressing the NF-κB signaling pathway. Furthermore, LSF could upregulate Bcl-2 and downregulate Bax, Caspase-3, and cleaved-PARP protein expressions. Taken together, our results first demonstrated that LSF could suppress the inflammatory response via inhibiting NF-κB signaling pathway, and inhibit apoptosis in Aβ(1-42)-induced BV-2 cells. Our findings further prove that LSF as a potential drug may be used for treating AD in the future. Frontiers Media S.A. 2018-04-23 /pmc/articles/PMC5925968/ /pubmed/29740316 http://dx.doi.org/10.3389/fphar.2018.00380 Text en Copyright © 2018 Zhao, Zeng, Wu, Yu, Tang, Wang, Xiong, Chen, Wu and Qin. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Zhao, Ya Zeng, Yuan Wu, Anguo Yu, Chonglin Tang, Yong Wang, Xiuling Xiong, Rui Chen, Haixia Wu, Jianming Qin, Dalian Lychee Seed Fraction Inhibits Aβ(1-42)-Induced Neuroinflammation in BV-2 Cells via NF-κB Signaling Pathway |
title | Lychee Seed Fraction Inhibits Aβ(1-42)-Induced Neuroinflammation in BV-2 Cells via NF-κB Signaling Pathway |
title_full | Lychee Seed Fraction Inhibits Aβ(1-42)-Induced Neuroinflammation in BV-2 Cells via NF-κB Signaling Pathway |
title_fullStr | Lychee Seed Fraction Inhibits Aβ(1-42)-Induced Neuroinflammation in BV-2 Cells via NF-κB Signaling Pathway |
title_full_unstemmed | Lychee Seed Fraction Inhibits Aβ(1-42)-Induced Neuroinflammation in BV-2 Cells via NF-κB Signaling Pathway |
title_short | Lychee Seed Fraction Inhibits Aβ(1-42)-Induced Neuroinflammation in BV-2 Cells via NF-κB Signaling Pathway |
title_sort | lychee seed fraction inhibits aβ(1-42)-induced neuroinflammation in bv-2 cells via nf-κb signaling pathway |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5925968/ https://www.ncbi.nlm.nih.gov/pubmed/29740316 http://dx.doi.org/10.3389/fphar.2018.00380 |
work_keys_str_mv | AT zhaoya lycheeseedfractioninhibitsab142inducedneuroinflammationinbv2cellsvianfkbsignalingpathway AT zengyuan lycheeseedfractioninhibitsab142inducedneuroinflammationinbv2cellsvianfkbsignalingpathway AT wuanguo lycheeseedfractioninhibitsab142inducedneuroinflammationinbv2cellsvianfkbsignalingpathway AT yuchonglin lycheeseedfractioninhibitsab142inducedneuroinflammationinbv2cellsvianfkbsignalingpathway AT tangyong lycheeseedfractioninhibitsab142inducedneuroinflammationinbv2cellsvianfkbsignalingpathway AT wangxiuling lycheeseedfractioninhibitsab142inducedneuroinflammationinbv2cellsvianfkbsignalingpathway AT xiongrui lycheeseedfractioninhibitsab142inducedneuroinflammationinbv2cellsvianfkbsignalingpathway AT chenhaixia lycheeseedfractioninhibitsab142inducedneuroinflammationinbv2cellsvianfkbsignalingpathway AT wujianming lycheeseedfractioninhibitsab142inducedneuroinflammationinbv2cellsvianfkbsignalingpathway AT qindalian lycheeseedfractioninhibitsab142inducedneuroinflammationinbv2cellsvianfkbsignalingpathway |