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Peroxisome Proliferator‐activated Receptor γ Induces Growth Arrest and Differentiation Markers of Human Colon Cancer Cells

Peroxisome proliferator‐activated receptor γ (PPARγ), one of the nuclear receptors expressed in adipose tissue, plays an important role in adipocyte differentiation. In this study, we investigated the expression of PPARγ and its role in cellular growth and differentiation in six colon cancer cell li...

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Autores principales: Kitamura, Shinji, Miyazaki, Yoshiji, Shinomura, Yasuhisa, Kondo, Shinya, Kanayama, Shuji, Matsuzawa, Yuji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1999
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5925976/
https://www.ncbi.nlm.nih.gov/pubmed/10076568
http://dx.doi.org/10.1111/j.1349-7006.1999.tb00668.x
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author Kitamura, Shinji
Miyazaki, Yoshiji
Shinomura, Yasuhisa
Kondo, Shinya
Kanayama, Shuji
Matsuzawa, Yuji
author_facet Kitamura, Shinji
Miyazaki, Yoshiji
Shinomura, Yasuhisa
Kondo, Shinya
Kanayama, Shuji
Matsuzawa, Yuji
author_sort Kitamura, Shinji
collection PubMed
description Peroxisome proliferator‐activated receptor γ (PPARγ), one of the nuclear receptors expressed in adipose tissue, plays an important role in adipocyte differentiation. In this study, we investigated the expression of PPARγ and its role in cellular growth and differentiation in six colon cancer cell lines: HT‐29, CaCo‐2, SW‐480, DLD‐1, LoVo, and T‐84. All six expressed PPARγ mRNA and protein, shown respectively on northern and western blot analyses. Luciferase assay in HT‐29 cells, which strongly express PPARγ showed that troglitazone, a selective ligand for PPAR?, transacti‐vated the transcription of a peroxisome proliferator response element (PPRE)‐driven promoter. Furthermore, troglitazone caused a marked decrease in [(3)H] thymidine incorporation and G1 cell‐cycle arrest determined by flow cytometry. Finally, troglitazone induced expression of mRNAs for villin and intestinal alkaline phosphatase, markers for enterocyte differentiation. In conclusion, human colon cancer cells express PPARγ, the ligands of which inhibit cell growth and induce differentiation markers.
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spelling pubmed-59259762018-05-11 Peroxisome Proliferator‐activated Receptor γ Induces Growth Arrest and Differentiation Markers of Human Colon Cancer Cells Kitamura, Shinji Miyazaki, Yoshiji Shinomura, Yasuhisa Kondo, Shinya Kanayama, Shuji Matsuzawa, Yuji Jpn J Cancer Res Article Peroxisome proliferator‐activated receptor γ (PPARγ), one of the nuclear receptors expressed in adipose tissue, plays an important role in adipocyte differentiation. In this study, we investigated the expression of PPARγ and its role in cellular growth and differentiation in six colon cancer cell lines: HT‐29, CaCo‐2, SW‐480, DLD‐1, LoVo, and T‐84. All six expressed PPARγ mRNA and protein, shown respectively on northern and western blot analyses. Luciferase assay in HT‐29 cells, which strongly express PPARγ showed that troglitazone, a selective ligand for PPAR?, transacti‐vated the transcription of a peroxisome proliferator response element (PPRE)‐driven promoter. Furthermore, troglitazone caused a marked decrease in [(3)H] thymidine incorporation and G1 cell‐cycle arrest determined by flow cytometry. Finally, troglitazone induced expression of mRNAs for villin and intestinal alkaline phosphatase, markers for enterocyte differentiation. In conclusion, human colon cancer cells express PPARγ, the ligands of which inhibit cell growth and induce differentiation markers. Blackwell Publishing Ltd 1999-01 /pmc/articles/PMC5925976/ /pubmed/10076568 http://dx.doi.org/10.1111/j.1349-7006.1999.tb00668.x Text en
spellingShingle Article
Kitamura, Shinji
Miyazaki, Yoshiji
Shinomura, Yasuhisa
Kondo, Shinya
Kanayama, Shuji
Matsuzawa, Yuji
Peroxisome Proliferator‐activated Receptor γ Induces Growth Arrest and Differentiation Markers of Human Colon Cancer Cells
title Peroxisome Proliferator‐activated Receptor γ Induces Growth Arrest and Differentiation Markers of Human Colon Cancer Cells
title_full Peroxisome Proliferator‐activated Receptor γ Induces Growth Arrest and Differentiation Markers of Human Colon Cancer Cells
title_fullStr Peroxisome Proliferator‐activated Receptor γ Induces Growth Arrest and Differentiation Markers of Human Colon Cancer Cells
title_full_unstemmed Peroxisome Proliferator‐activated Receptor γ Induces Growth Arrest and Differentiation Markers of Human Colon Cancer Cells
title_short Peroxisome Proliferator‐activated Receptor γ Induces Growth Arrest and Differentiation Markers of Human Colon Cancer Cells
title_sort peroxisome proliferator‐activated receptor γ induces growth arrest and differentiation markers of human colon cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5925976/
https://www.ncbi.nlm.nih.gov/pubmed/10076568
http://dx.doi.org/10.1111/j.1349-7006.1999.tb00668.x
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