Detection of Methylation Damage in DNA of Gastric Cancer Tissues Using (32)P‐Postlabelling Assay

Gastric cancer is the most common cancer in Korea. The causes are still unknown but it has been speculated that gastric cancer is associated with consumption of foods rich in nitrates/nitrites or a high dietary intake of salt or pickled food. In the present study, we studied the level of alkylated D...

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Autores principales: Kim, Dae‐Yong, Cho, Myung‐Haing, Yang, Han‐Kwang, Hemminki, Kari, Kim, Jin‐Pok, Jang, Ja‐June, Kumar, Rajiv
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1999
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5925992/
https://www.ncbi.nlm.nih.gov/pubmed/10595739
http://dx.doi.org/10.1111/j.1349-7006.1999.tb00684.x
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author Kim, Dae‐Yong
Cho, Myung‐Haing
Yang, Han‐Kwang
Hemminki, Kari
Kim, Jin‐Pok
Jang, Ja‐June
Kumar, Rajiv
author_facet Kim, Dae‐Yong
Cho, Myung‐Haing
Yang, Han‐Kwang
Hemminki, Kari
Kim, Jin‐Pok
Jang, Ja‐June
Kumar, Rajiv
author_sort Kim, Dae‐Yong
collection PubMed
description Gastric cancer is the most common cancer in Korea. The causes are still unknown but it has been speculated that gastric cancer is associated with consumption of foods rich in nitrates/nitrites or a high dietary intake of salt or pickled food. In the present study, we studied the level of alkylated DNA adducts formed in gastric cancer tissues in comparison with that in normal gastric mucosa. DNA was extracted from surgically removed gastric cancer tissues and patient‐matched normal gastric mucosa. The level of N(7)‐methyldeoxyguanosine was measured by (32)P‐postlabelling assay after high performance liquid chromatography (HPLC) enrichment. We found that the level of N(7)‐methyldeoxyguanosine of gastric cancerous tissues was significantly higher than that of normal gastric mucosa (P=0.01685).
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spelling pubmed-59259922018-05-11 Detection of Methylation Damage in DNA of Gastric Cancer Tissues Using (32)P‐Postlabelling Assay Kim, Dae‐Yong Cho, Myung‐Haing Yang, Han‐Kwang Hemminki, Kari Kim, Jin‐Pok Jang, Ja‐June Kumar, Rajiv Jpn J Cancer Res Article Gastric cancer is the most common cancer in Korea. The causes are still unknown but it has been speculated that gastric cancer is associated with consumption of foods rich in nitrates/nitrites or a high dietary intake of salt or pickled food. In the present study, we studied the level of alkylated DNA adducts formed in gastric cancer tissues in comparison with that in normal gastric mucosa. DNA was extracted from surgically removed gastric cancer tissues and patient‐matched normal gastric mucosa. The level of N(7)‐methyldeoxyguanosine was measured by (32)P‐postlabelling assay after high performance liquid chromatography (HPLC) enrichment. We found that the level of N(7)‐methyldeoxyguanosine of gastric cancerous tissues was significantly higher than that of normal gastric mucosa (P=0.01685). Blackwell Publishing Ltd 1999-10 /pmc/articles/PMC5925992/ /pubmed/10595739 http://dx.doi.org/10.1111/j.1349-7006.1999.tb00684.x Text en
spellingShingle Article
Kim, Dae‐Yong
Cho, Myung‐Haing
Yang, Han‐Kwang
Hemminki, Kari
Kim, Jin‐Pok
Jang, Ja‐June
Kumar, Rajiv
Detection of Methylation Damage in DNA of Gastric Cancer Tissues Using (32)P‐Postlabelling Assay
title Detection of Methylation Damage in DNA of Gastric Cancer Tissues Using (32)P‐Postlabelling Assay
title_full Detection of Methylation Damage in DNA of Gastric Cancer Tissues Using (32)P‐Postlabelling Assay
title_fullStr Detection of Methylation Damage in DNA of Gastric Cancer Tissues Using (32)P‐Postlabelling Assay
title_full_unstemmed Detection of Methylation Damage in DNA of Gastric Cancer Tissues Using (32)P‐Postlabelling Assay
title_short Detection of Methylation Damage in DNA of Gastric Cancer Tissues Using (32)P‐Postlabelling Assay
title_sort detection of methylation damage in dna of gastric cancer tissues using (32)p‐postlabelling assay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5925992/
https://www.ncbi.nlm.nih.gov/pubmed/10595739
http://dx.doi.org/10.1111/j.1349-7006.1999.tb00684.x
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