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Epidermal Growth Factor‐dependent Dissociation of CrkII Proto‐oncogene Product from the Epidermal Growth Factor Receptor in Human Glioma Cells

Human glioma cells frequently overexpress epidermal growth factor receptor (EGFR). We found that the CrkII proto‐oncogene product was associated with the EGFR in human glioma cells in the absence of epidermal growth factor (EGF). EGF stimulation of glioma cells induced the phosphorylation of tyrosin...

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Autores principales: Katayama, Haruko, Hashimoto, Yuko, Kiyokawa, Etsuko, Nakaya, Mie, Sakamoto, Atsuhiko, Machinami, Rikuo, Kurata, Takeshi, Mochizuki, Naoki, Matsuda, Michiyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1999
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926002/
https://www.ncbi.nlm.nih.gov/pubmed/10595738
http://dx.doi.org/10.1111/j.1349-7006.1999.tb00683.x
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author Katayama, Haruko
Hashimoto, Yuko
Kiyokawa, Etsuko
Nakaya, Mie
Sakamoto, Atsuhiko
Machinami, Rikuo
Kurata, Takeshi
Mochizuki, Naoki
Matsuda, Michiyuki
author_facet Katayama, Haruko
Hashimoto, Yuko
Kiyokawa, Etsuko
Nakaya, Mie
Sakamoto, Atsuhiko
Machinami, Rikuo
Kurata, Takeshi
Mochizuki, Naoki
Matsuda, Michiyuki
author_sort Katayama, Haruko
collection PubMed
description Human glioma cells frequently overexpress epidermal growth factor receptor (EGFR). We found that the CrkII proto‐oncogene product was associated with the EGFR in human glioma cells in the absence of epidermal growth factor (EGF). EGF stimulation of glioma cells induced the phosphorylation of tyrosine 221 of the CrkII protein, which correlates with its dissociation from the EGFR. By contrast, Shc and Grb2 were inducibly associated with the EGFR in response to EGF stimulation of glioma cells. In A431 cells, epidermoid carcinoma cells which overexpress EGFR, CrkII was tyrosine‐phosphorylated and associated with the EGFR in an EGF‐dependent manner. Therefore, the dissociation of CrkII from the EGFR upon stimulation with EGF appears to be specific to glioma cells. The Cbl oncogene product was also tyrosine‐phosphorylated in U87MG glioma cells upon EGF stimulation. However, unlike in other cell lines, CrkII was not inducibly bound to Cbl in U87MG glioma cells. Thus, EGF‐dependent binding of CrkII to phosphotyrosine‐containing proteins appears to be suppressed in glioma cells. To evaluate the physiological role of dissociation of CrkII from EGFR, we expressed the CrkII‐23 mutant in glioma cells. CrkII‐23 mutant, which was isolated as a suppressor gene of the EGF‐dependent transformation of NRK cells, binds constitutively to EGFR. We found that expression of CrkII‐23 inhibited the anchorage‐independent growth of the glioma cells in the presence of EGF. Taken together, these data implicate EGF‐dependent dissociation of CrkII from EGFR in the oncogenicity of human glioma cells.
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spelling pubmed-59260022018-05-11 Epidermal Growth Factor‐dependent Dissociation of CrkII Proto‐oncogene Product from the Epidermal Growth Factor Receptor in Human Glioma Cells Katayama, Haruko Hashimoto, Yuko Kiyokawa, Etsuko Nakaya, Mie Sakamoto, Atsuhiko Machinami, Rikuo Kurata, Takeshi Mochizuki, Naoki Matsuda, Michiyuki Jpn J Cancer Res Article Human glioma cells frequently overexpress epidermal growth factor receptor (EGFR). We found that the CrkII proto‐oncogene product was associated with the EGFR in human glioma cells in the absence of epidermal growth factor (EGF). EGF stimulation of glioma cells induced the phosphorylation of tyrosine 221 of the CrkII protein, which correlates with its dissociation from the EGFR. By contrast, Shc and Grb2 were inducibly associated with the EGFR in response to EGF stimulation of glioma cells. In A431 cells, epidermoid carcinoma cells which overexpress EGFR, CrkII was tyrosine‐phosphorylated and associated with the EGFR in an EGF‐dependent manner. Therefore, the dissociation of CrkII from the EGFR upon stimulation with EGF appears to be specific to glioma cells. The Cbl oncogene product was also tyrosine‐phosphorylated in U87MG glioma cells upon EGF stimulation. However, unlike in other cell lines, CrkII was not inducibly bound to Cbl in U87MG glioma cells. Thus, EGF‐dependent binding of CrkII to phosphotyrosine‐containing proteins appears to be suppressed in glioma cells. To evaluate the physiological role of dissociation of CrkII from EGFR, we expressed the CrkII‐23 mutant in glioma cells. CrkII‐23 mutant, which was isolated as a suppressor gene of the EGF‐dependent transformation of NRK cells, binds constitutively to EGFR. We found that expression of CrkII‐23 inhibited the anchorage‐independent growth of the glioma cells in the presence of EGF. Taken together, these data implicate EGF‐dependent dissociation of CrkII from EGFR in the oncogenicity of human glioma cells. Blackwell Publishing Ltd 1999-10 /pmc/articles/PMC5926002/ /pubmed/10595738 http://dx.doi.org/10.1111/j.1349-7006.1999.tb00683.x Text en
spellingShingle Article
Katayama, Haruko
Hashimoto, Yuko
Kiyokawa, Etsuko
Nakaya, Mie
Sakamoto, Atsuhiko
Machinami, Rikuo
Kurata, Takeshi
Mochizuki, Naoki
Matsuda, Michiyuki
Epidermal Growth Factor‐dependent Dissociation of CrkII Proto‐oncogene Product from the Epidermal Growth Factor Receptor in Human Glioma Cells
title Epidermal Growth Factor‐dependent Dissociation of CrkII Proto‐oncogene Product from the Epidermal Growth Factor Receptor in Human Glioma Cells
title_full Epidermal Growth Factor‐dependent Dissociation of CrkII Proto‐oncogene Product from the Epidermal Growth Factor Receptor in Human Glioma Cells
title_fullStr Epidermal Growth Factor‐dependent Dissociation of CrkII Proto‐oncogene Product from the Epidermal Growth Factor Receptor in Human Glioma Cells
title_full_unstemmed Epidermal Growth Factor‐dependent Dissociation of CrkII Proto‐oncogene Product from the Epidermal Growth Factor Receptor in Human Glioma Cells
title_short Epidermal Growth Factor‐dependent Dissociation of CrkII Proto‐oncogene Product from the Epidermal Growth Factor Receptor in Human Glioma Cells
title_sort epidermal growth factor‐dependent dissociation of crkii proto‐oncogene product from the epidermal growth factor receptor in human glioma cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926002/
https://www.ncbi.nlm.nih.gov/pubmed/10595738
http://dx.doi.org/10.1111/j.1349-7006.1999.tb00683.x
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