Cargando…

Immunohistochemical Localization of Glutathione S‐Transferase α and π in Human Esophageal Squamous Epithelium, Barrett's Epithelium and Carcinoma

High tissue levels of glutathione S‐transferases (GSTs), a family of detoxification enzymes, are inversely correlated with cancer risk in the human gastrointestinal tract. Patients with Barrett's esophagus, wherein squamous epithelium is replaced by columnar epithelium, have an increased risk f...

Descripción completa

Detalles Bibliográficos
Autores principales: van Lieshout, Esther M. M., van Haelst, Urbain J. G. M., Wobbes, Theo, Peters, Wilbert H. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1999
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926098/
https://www.ncbi.nlm.nih.gov/pubmed/10391093
http://dx.doi.org/10.1111/j.1349-7006.1999.tb00780.x
_version_ 1783318836693434368
author van Lieshout, Esther M. M.
van Haelst, Urbain J. G. M.
Wobbes, Theo
Peters, Wilbert H. M.
author_facet van Lieshout, Esther M. M.
van Haelst, Urbain J. G. M.
Wobbes, Theo
Peters, Wilbert H. M.
author_sort van Lieshout, Esther M. M.
collection PubMed
description High tissue levels of glutathione S‐transferases (GSTs), a family of detoxification enzymes, are inversely correlated with cancer risk in the human gastrointestinal tract. Patients with Barrett's esophagus, wherein squamous epithelium is replaced by columnar epithelium, have an increased risk for developing esophageal adenocarcinoma. Biochemical analyses revealed that Barrett's epithelium contains lower levels of GST enzyme activity as well as some GST isoforms, as compared with squamous epithelium. So far, little information on the immunohistochemical distribution of the GST α and π isoforms in normal squamous epithelium, in Barrett's metaplastic epithelium or in adeno‐ and squamous cell carcinomas of the esophagus is available. Tissues were fixed in formalin and embedded in paraffin. Three 4 μm thick sections were used for hematoxylin and eosin staining and for immunostaining with antibodies against GST α and π. GST α and π were seen in normal squamous epithelium (0% and 75%, respectively), Barrett's epithelium (75% and 100%), adenocarcinoma (25% and 100%) and squamous cell carcinoma (27% and 91%). Staining was mainly cytoplasmic, though some nuclear staining with the GST π antibody was apparent. The varying expression of GST α and π in normal and (pre)neoplastic esophagus may have consequences for the treatment of these diseases and may contribute to an understanding of the development of these esophageal disorders.
format Online
Article
Text
id pubmed-5926098
institution National Center for Biotechnology Information
language English
publishDate 1999
publisher Blackwell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-59260982018-05-11 Immunohistochemical Localization of Glutathione S‐Transferase α and π in Human Esophageal Squamous Epithelium, Barrett's Epithelium and Carcinoma van Lieshout, Esther M. M. van Haelst, Urbain J. G. M. Wobbes, Theo Peters, Wilbert H. M. Jpn J Cancer Res Article High tissue levels of glutathione S‐transferases (GSTs), a family of detoxification enzymes, are inversely correlated with cancer risk in the human gastrointestinal tract. Patients with Barrett's esophagus, wherein squamous epithelium is replaced by columnar epithelium, have an increased risk for developing esophageal adenocarcinoma. Biochemical analyses revealed that Barrett's epithelium contains lower levels of GST enzyme activity as well as some GST isoforms, as compared with squamous epithelium. So far, little information on the immunohistochemical distribution of the GST α and π isoforms in normal squamous epithelium, in Barrett's metaplastic epithelium or in adeno‐ and squamous cell carcinomas of the esophagus is available. Tissues were fixed in formalin and embedded in paraffin. Three 4 μm thick sections were used for hematoxylin and eosin staining and for immunostaining with antibodies against GST α and π. GST α and π were seen in normal squamous epithelium (0% and 75%, respectively), Barrett's epithelium (75% and 100%), adenocarcinoma (25% and 100%) and squamous cell carcinoma (27% and 91%). Staining was mainly cytoplasmic, though some nuclear staining with the GST π antibody was apparent. The varying expression of GST α and π in normal and (pre)neoplastic esophagus may have consequences for the treatment of these diseases and may contribute to an understanding of the development of these esophageal disorders. Blackwell Publishing Ltd 1999-05 /pmc/articles/PMC5926098/ /pubmed/10391093 http://dx.doi.org/10.1111/j.1349-7006.1999.tb00780.x Text en
spellingShingle Article
van Lieshout, Esther M. M.
van Haelst, Urbain J. G. M.
Wobbes, Theo
Peters, Wilbert H. M.
Immunohistochemical Localization of Glutathione S‐Transferase α and π in Human Esophageal Squamous Epithelium, Barrett's Epithelium and Carcinoma
title Immunohistochemical Localization of Glutathione S‐Transferase α and π in Human Esophageal Squamous Epithelium, Barrett's Epithelium and Carcinoma
title_full Immunohistochemical Localization of Glutathione S‐Transferase α and π in Human Esophageal Squamous Epithelium, Barrett's Epithelium and Carcinoma
title_fullStr Immunohistochemical Localization of Glutathione S‐Transferase α and π in Human Esophageal Squamous Epithelium, Barrett's Epithelium and Carcinoma
title_full_unstemmed Immunohistochemical Localization of Glutathione S‐Transferase α and π in Human Esophageal Squamous Epithelium, Barrett's Epithelium and Carcinoma
title_short Immunohistochemical Localization of Glutathione S‐Transferase α and π in Human Esophageal Squamous Epithelium, Barrett's Epithelium and Carcinoma
title_sort immunohistochemical localization of glutathione s‐transferase α and π in human esophageal squamous epithelium, barrett's epithelium and carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926098/
https://www.ncbi.nlm.nih.gov/pubmed/10391093
http://dx.doi.org/10.1111/j.1349-7006.1999.tb00780.x
work_keys_str_mv AT vanlieshoutesthermm immunohistochemicallocalizationofglutathionestransferaseaandpinhumanesophagealsquamousepitheliumbarrettsepitheliumandcarcinoma
AT vanhaelsturbainjgm immunohistochemicallocalizationofglutathionestransferaseaandpinhumanesophagealsquamousepitheliumbarrettsepitheliumandcarcinoma
AT wobbestheo immunohistochemicallocalizationofglutathionestransferaseaandpinhumanesophagealsquamousepitheliumbarrettsepitheliumandcarcinoma
AT peterswilberthm immunohistochemicallocalizationofglutathionestransferaseaandpinhumanesophagealsquamousepitheliumbarrettsepitheliumandcarcinoma